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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD3 Antibodies

          Invitrogen

          CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™

          Advanced Verification
          97 Published Figures
          212 References
          View all (543) CD3 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (97)
          • Advanced Verification (1)
          CD3e Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD3e Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 99

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          CD3e Antibody (12-0031-82) in Flow

          Staining of C57Bl/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD3e PE (purple histogram). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody in Flow Cytometry (Flow)
          CD3e Antibody

          Product Details

          12-0031-82

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          0.5 µg/test
          View 208 publications 208 publications

          Functional Assay (Functional)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          145-2C11

          Conjugate

          PE PE PE
          • Unconjugated
          • Alexa Fluor 488 
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 496
          • Brilliant UV 563
          • Brilliant UV 615
          • Brilliant UV 661
          • Brilliant UV 737
          • Brilliant Violet 421
          • Brilliant Violet 480
          • Brilliant Violet 605
          • Brilliant Violet 650
          • Brilliant Violet 711
          • Brilliant Violet 786
          • eFluor 450
          • FITC
          • Functional Grade
          • NovaFluor Blue 510
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Red 685
          • NovaFluor Red 700
          • NovaFluor Red 710
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 690
          • NovaFluor Yellow 700
          • NovaFluor Yellow 730
          • PE-Cyanine5
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Super Bright 780
          • Request custom conjugation

          Excitation/Emission Max

          565/576 nm View spectra spectra

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_465496

          Product Specific Information

          Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

          Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

          View more View less

          Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

          View more View less

          UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

          View more View less

          Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

          View more View less

          Function(s)
          transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding defense/immunity protein immunoglobulin receptor superfamily
          Process(es)
          cell surface receptor signaling pathway T cell receptor signaling pathway protein complex assembly T cell activation positive thymic T cell selection positive regulation of T cell anergy response to nutrient T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation lymphocyte activation positive regulation of peptidyl-tyrosine phosphorylation positive regulation of calcium-mediated signaling positive regulation of T cell activation apoptotic signaling pathway establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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