Invitrogen

CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™

40 Published Figures

143 References

View all (227) CD3 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

CD3e Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD3e PE (purple histogram). Total viable cells were used for analysis.

CD3e Antibody — Figure 5 Tpl2 mediated the liver recruitment of MDSC during FH pathogenesis. (A,B,D-G) Flow cytometric analysis of Gr-1 + CD11b + MDSC in the livers (A,B) or spleens (D,E) or peripheral blood (F,G ) of WT and Tpl2 -KO mice at day 3, 5, and 7 after P. acnes priming ( n = 4 mice/group). Data are presented as representative plots (A,D,F) and summary bar graph (B,E,G) showing the absolute numbers or frequencies of MDSC. (C) Immunofluorescence images showing the infiltrated MDSC by using the anti-Ly6G antibody in the liver sections obtained from WT and Tpl2 -KO mice at day 7 after P. acnes priming (magnification, x200). (H,I) Flow cytometry analysis of the proliferation of WT CD4 + T cells that labeled with CFSE, and then cocultured with WT or Tpl2 -KO MDSC at the indicated ratio in the absence (NT) or presence of anti-CD3/28 antibodies for 72 h. Data are presented representative histograms (H) and bar graph (I) . (J) Cumulative survival rates of WT and Tpl2 -KO mice that injected with anti-Ly6G antibody (200 mug/mouse, three times) to deplete in vivo MDSC or control antibody, and then subjected to P. acnes /LPS-mediated FH induction ( n = 4 mice/group). Results are mean +- SD from three independent experiments. Two-tailed Student's t -tests were performed. * P < 0.05; ** P < 0.01.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 138 publications 138 publications

Immunofluorescence (IF)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Functional Assay (FN)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

145-2C11

Conjugate

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_465496

Product Specific Information

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

Gene Aliases: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor