Invitrogen

CD3e Monoclonal Antibody (145-2C11), PE, eBioscience™

Product Image Gallery

CD3e Antibody — Figure 2 Characterization of spleens from WT and Ats1-KO mice. (a) Graph representing the spleen index (as indicated in the Methods section) of healthy WT (n = 6) and Ats1-KO (n = 5) mice to assess splenomegaly. Inset below include representative images of spleens from WT and Ats1-KO mice (white scale bar = 1 cm). (b) Graph representing flow cytometry data, as percentage of positive cells of the following populations: CD45R + , CD3 + , CD11b + , GR1 + , CD11b + /GR1 + , and F4/80 + , found in spleens of healthy WT (n = 5) and Ats1-KO (n = 5) mice. (c) Graph representing the mRNA fold change expression of Cd3g , Cd4 , Il6 , Il10 and Il12a genes in spleens of healthy WT (n = 5) and Ats1-KO (n = 5) mice, being all values relative to WT. (d) Representative images of spleen sections from WT and Ats1-KO mice, showing VCAN and VKIN (red) (left and right column, respectively), and CD31 (green) immunofluorescence staining. Images correspond to a 63x magnification (white scale bar = 20 mum). (e) Western blot analysis with anti-DPEAAE antibody for ADAMTS-cleaved versican in spleen protein extracts from WT and Ats1-KO mice. Blue arrow indicates a predicted versican (V0) fragment (around 220 kDa). Bottom panel includes actin staining (grey arrow). Full-length blots are presented in Supplementary Figure 4. (f) Graph representing the absolute Ct value for mRNA expression of Adamts1 , 4 , 5 , 9 , 15 , and 20 genes in spleens of healthy WT (n = 5) and Ats1-KO (n = 4)

CD3e Antibody in Flow Cytometry (Flow) — Staining of C57Bl/6 splenocytes with 0.25 µg of Armenian Hamster IgG Isotype Control PE (Product # 12-4888-81) (blue histogram) or 0.25 µg of Anti-Mouse CD3e PE (purple histogram). Total viable cells were used for analysis.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

0.5 µg/test

Published Applications

Flow Cytometry (Flow)

See 118 publications below

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 3 publications below

Functional Assay (FN)

See 1 publication below

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host / Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

145-2C11

Conjugate

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_465496

Product Specific Information

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD3-epsilon; CD3e; CD3e antigen; epsilon polypeptide TiT3 complex; epsilon subunit of T3; FLJ18683; T-cell antigen receptor complex; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE

Gene Aliases: AI504783; CD3; Cd3e; CD3epsilon; T3e

UniProt ID: (Mouse) P22646

Entrez Gene ID: (Mouse) 12501

Molecular Function: cytokine receptor defense/immunity protein immunoglobulin receptor superfamily receptor