Invitrogen

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Functional Grade, eBioscience™

Product Image Gallery

CD62L (L-Selectin) Antibody in Flow Cytometry (Flow) — Staining of normal human peripheral blood cells with Anti-Human CD62L (L-Selectin) FITC (left) or APC (right).Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

Product Details

16-0629-85

Applications	Tested Dilution	Publications
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Flow Cytometry (Flow)	1 µg/test	View 13 publications 13 publications
Neutralization (Neu)	Assay-Dependent	-
Functional Assay (FN)	Assay-Dependent	-

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	DREG-56 (DREG56)
Conjugate	Functional Grade Alexa Fluor 532 APC APC-eFluor 780 Brilliant UV 496 Brilliant UV 615 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 711 Brilliant Violet 786 eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 660-40S NovaFluor Blue 690 NovaFluor Red 710 NovaFluor Red 725 NovaFluor Red 755 NovaFluor Yell
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	no preservative
Storage conditions	4° C
RRID	AB_468992

Product Specific Information

Description: The DREG-56 monoclonal antibody reacts with human CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCAM-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported: The DREG-56 (DREG56) antibody has been reported for use in flow cytometric analysis. DREG-56 has also been reported in inhibition of binding to HEV.

Applications Tested: The DREG-56 (DREG56) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

The human CD62L is a 74-95 kDa glycoprotein member of the selectin family of adhesion receptors. L-Selectin is comprised of an aminoterminal C-type lectin binding domain, an epidermal growth factor-like domain, two short consensus repeat (SCR) sequences homologous to those found in complement binding proteins, a short spacer region, a transmembrane region and a short cytoplasmic region. Human CD62L (L-Selectin) is constitutively expressed on all classes of leukocytes including lymphocytes (except a substantial population of memory T cells), monocytes and polymorphonuclear cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Induction of memory-like CD8+ T cells and CD4+ T cells from human naive T cells in culture.

Clinical and experimental immunology

Tokumoto Y,Araki Y,Narizuka Y,Mizuno Y,Ohshima S,Mimura T

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0629-85) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Blocking MHC class II on human endothelium mitigates acute rejection.

JCI insight

Abrahimi P,Qin L,Chang WG,Bothwell AL,Tellides G,Saltzman WM,Pober JS

16-0629 was used in Flow cytometry/Cell sorting to establish the immune cell network involved in acute allograft rejection in humans and identify targets for minimising this rejection.

Wed Nov 20 00:00:00 EST 2019

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0629-85) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Characteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease.

International journal of immunopathology and pharmacology

Jung JY,Choi B,Sayeed HM,Suh CH,Kim YW,Kim HA,Sohn S

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0629-85) in Flow Cytometry

Tue Dec 18 00:00:00 EST 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0629-85) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.

The Journal of clinical investigation

Rathod KS,Kapil V,Velmurugan S,Khambata RS,Siddique U,Khan S,Van Eijl S,Gee LC,Bansal J,Pitrola K,Shaw C,D'Acquisto F,Colas RA,Marelli-Berg F,Dalli J,Ahluwalia A

Published figure using CD62L (L-Selectin) monoclonal antibody (Product # 16-0629-85) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2017

Peptide-induced immune regulation by a promiscuous and immunodominant CD4T-cell epitope of Timothy grass pollen: a role of Cbl-b and Itch in regulation.

Thorax

Till SJ,Raynsford EJ,Reynolds CJ,Quigley KJ,Grzybowska-Kowalczyk A,Saggar LR,Goldstone A,Maillere B,Kwok WW,Altmann DM,Durham SR,Boyton RJ

16-0629 was used in Flow cytometry/Cell sorting to identify human leukocyte antigen-DR1-restricted epitopes from the Timothy grass pollen allergen, Phleum pratense, and characterise T-cell immune regulation following intranasal administration of a single, immunodominant epitope.

Tue Apr 01 00:00:00 EDT 2014

Novel serial positive enrichment technology enables clinical multiparameter cell sorting.

PloS one

Stemberger C,Dreher S,Tschulik C,Piossek C,Bet J,Yamamoto TN,Schiemann M,Neuenhahn M,Martin K,Schlapschy M,Skerra A,Schmidt T,Edinger M,Riddell SR,Germeroth L,Busch DH

16-0629 was used in Flow cytometry/Cell sorting to exhibit novel, low-affinity antibody-derived Fab-fragments for use in clinical cell preparations and research.

Mon Aug 27 00:00:00 EDT 2012

Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.

Arteriosclerosis, thrombosis, and vascular biology

Norling LV,Dalli J,Flower RJ,Serhan CN,Perretti M

16-0629 was used in Flow cytometry/Cell sorting to study the role of Resolvin D1 in limiting neutrophil recruitment during acute inflammation.

Wed Aug 01 00:00:00 EDT 2012

Comparison of gene expression profiles between human and mouse monocyte subsets.

Blood

Ingersoll MA,Spanbroek R,Lottaz C,Gautier EL,Frankenberger M,Hoffmann R,Lang R,Haniffa M,Collin M,Tacke F,Habenicht AJ,Ziegler-Heitbrock L,Randolph GJ

16-0629 was used in Flow cytometry/Cell sorting to identify how human and mouse monocyte subsets are far more broadly conserved than currently recognised.

Thu Jan 21 00:00:00 EST 2010

Image correlation microscopy for uniform illumination.

Journal of microscopy

Gaborski TR,Sealander MN,Ehrenberg M,Waugh RE,McGrath JL

16-0629 was used in Immunohistochemistry to exhibit a novel use for image cross-correlation microscopy.

Fri Jan 01 00:00:00 EST 2010

Membrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils.

Biophysical journal

Gaborski TR,Clark A,Waugh RE,McGrath JL

16-0629 was used in Immunofluorescence to present an analytical model to measure the mobility of the four key receptors involved in the neutrophil adhesion cascade.

Sat Nov 15 00:00:00 EST 2008

PILAR is a novel modulator of human T-cell expansion.

Blood

Huarte E,Cubillos-Ruiz JR,Nesbeth YC,Scarlett UK,Martinez DG,Engle XA,Rigby WF,Pioli PA,Guyre PM,Conejo-Garcia JR

16-0629 was used in Flow cytometry/Cell sorting to identify a novel human transmembrane molecule expressed on T cells that enhances their expansion through CD161.

Fri Aug 15 00:00:00 EDT 2008

Monitoring receptor-ligand interactions between surfaces by thermal fluctuations.

Biophysical journal

Chen W,Evans EA,McEver RP,Zhu C

16-0629 was used in Flow cytometry/Cell sorting to develop and validate a method for monitoring 2D receptor-ligand interactions based on thermal fluctuations of a biomembrane force probe.

Tue Jan 15 00:00:00 EST 2008

Wiskott-Aldrich syndrome protein is required for regulatory T cell homeostasis.

The Journal of clinical investigation

Humblet-Baron S,Sather B,Anover S,Becker-Herman S,Kasprowicz DJ,Khim S,Nguyen T,Hudkins-Loya K,Alpers CE,Ziegler SF,Ochs H,Torgerson T,Campbell DJ,Rawlings DJ

16-0629 was used in Flow cytometry/Cell sorting to investigate whether alterations in Treg function might explain Wiskott-Aldrich syndrome pathology, showing that WASp is required for Treg homeostasis.

Thu Feb 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: CD62 antigen-like family member L; CD62L; gp90-MEL; hLHRc; L-selectin; LAM-1; LECAM1; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; TQ1

Gene Aliases: CD62L; LAM1; LECAM1; LEU8; LNHR; LSEL; LYAM1; PLNHR; SELL; TQ1

UniProt ID: (Human) P14151

Entrez Gene ID: (Human) 6402

Function(s)

cell adhesion response to ATP regulation of immune response leukocyte migration

Process(es)

protease binding protein binding heparin binding carbohydrate binding glycosphingolipid binding cell adhesion molecule binding

CD62L (L-Selectin) Monoclonal Antibody (DREG-56 (DREG56)), Functional Grade, eBioscience™

Product Details

16-0629-85

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (FN)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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