Invitrogen

CD69 Monoclonal Antibody (FN50), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

19 References

View all (57) CD69 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CD69 Antibody in Flow Cytometry (Flow) — Staining of PHA-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD69 FITC (filled histogram). Cells in the lymphocyte gate were used for analysis.

CD69 Antibody — Figure 7 Synergistic combinations avoid global T-cell activation and induction of cytokine release. Resting CD4+ CD25- T-cells were isolated from a HIV-1 negative donor blood. Cells were incubated 24 h with 1 nM bryologs and 100 nM HDAC inhibitor. Cells were stained with fluorescent-conjugated antibodies (a) CD25-APC and (b) CD69-FITC and analyzed by FACS (see Methods). Data indicate mean values and error bars indicate mean +- s.e.m. (n >= 3). ***p < 0.001, (ANOVA). (c) ELISA results for cytokine release from CD4+ CD25- T-cells following 24 h incubation with LRA combinations (see Methods). ( d ) Toxicity profile of LRA combinations. Resting CD4+ T-cells were incubated with compounds at indicated concentrations and MTT toxicity assay was performed as described in Methods. {TM}

Product Details

11-0699-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.125 µg)/test	View 19 publications 19 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	FN50
Conjugate	FITC Alexa Fluor 561 Alexa Fluor 660 APC APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_10853975

Product Specific Information

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

Applications Reported: The FN50 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

11-0699-42 was used in Flow Cytometry to characterise lipidation (S-acylation) of the ZAP-70 tyrosine kinase using Acyl-Biotin Exchange assay, a technique that selectively captures S-acylated proteins.

Thu Aug 26 00:00:00 EDT 2021

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Thu Aug 26 00:00:00 EDT 2021

Exosome-derived miR-142-5p remodels lymphatic vessels and induces IDO to promote immune privilege in the tumour microenvironment.

Cell death and differentiation

Zhou C,Zhang Y,Yan R,Huang L,Mellor AL,Yang Y,Chen X,Wei W,Wu X,Yu L,Liang L,Zhang D,Wu S,Wang W

11-0699 was used in Flow cytometry/Cell sorting to suggest that LECs act as an integral component of the immune checkpoint(s) in the TME and may serve as a potential new target for CSCC diagnosis and treatment.

Mon Feb 01 00:00:00 EST 2021

Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase.

Chinese medicine

Houng JY,Tai TS,Hsu SC,Hsu HF,Hwang TS,Lin CJ,Fang LW

Published figure using CD69 monoclonal antibody (Product # 11-0699-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Coagulation factor V is a marker of tumor-infiltrating immune cells in breast cancer.

Oncoimmunology

Tinholt M,Stavik B,Tekpli X,Garred Ø,Borgen E,Kristensen V,Sahlberg KK,Sandset PM,Iversen N

11-0699 was used in Flow cytometry/Cell sorting to identify F5 as a novel marker of immune cell infiltration in breast cancer, and verify the prognostic role of F5.

Tue Sep 29 00:00:00 EDT 2020

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD69 monoclonal antibody (Product # 11-0699-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Novel and shared neoantigen derived from histone 3 variant H3.3K27M mutation for glioma T cell therapy.

The Journal of experimental medicine

Chheda ZS,Kohanbash G,Okada K,Jahan N,Sidney J,Pecoraro M,Yang X,Carrera DA,Downey KM,Shrivastav S,Liu S,Lin Y,Lagisetti C,Chuntova P,Watchmaker PB,Mueller S,Pollack IF,Rajalingam R,Carcaboso AM,Mann M,Sette A,Garcia KC,Hou Y,Okada H

11-0699 was used in Flow cytometry/Cell sorting to assess whether targeting the neoantigen resulting from the histone 3 variant H3.3K27M mutation is therapeutically viable in T cell-based therapy for glioma.

Tue Jan 02 00:00:00 EST 2018

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Scientific reports

Albert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB

Mon Aug 07 00:00:00 EDT 2017

Chemical proteomic map of dimethyl fumarate-sensitive cysteines in primary human T cells.

Science signaling

Blewett MM,Xie J,Zaro BW,Backus KM,Altman A,Teijaro JR,Cravatt BF

11-0699 was used in Flow cytometry/Cell sorting to explore the mode of action of Dimethyl fumarate as a therapy for autoimmune diseases.

Tue Sep 13 00:00:00 EDT 2016

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

11-0699 was used in Flow cytometry/Cell sorting to demonstrate how MAIT cells are activated following viral infections, suggesting a potential role in both host defence and immunopathology.

Thu Jun 23 00:00:00 EDT 2016

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

11-0699 was used in Flow cytometry/Cell sorting to study RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Mon Dec 21 00:00:00 EST 2015

HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells.

eLife

Reeder JE,Kwak YT,McNamara RP,Forst CV,D'Orso I

Published figure using CD69 monoclonal antibody (Product # 11-0699-42) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2015

NK cell immunesenescence is increased by psychological but not physical stress in older adults associated with raised cortisol and reduced perforin expression.

Age (Dordrecht, Netherlands)

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

11-0699 was used in Flow cytometry/Cell sorting to assess the effect of the physical stress of hip fracture and the psychological stress of depression on NK cell cytotoxicity in an aged immune system.

Sun Feb 01 00:00:00 EST 2015

Depressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells.

Immunity & ageing : I & A

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

11-0699 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system.

Wed Jan 28 00:00:00 EST 2015

Endothelial microparticles interact with and support the proliferation of T cells.

Journal of immunology (Baltimore, Md. : 1950)

Wheway J,Latham SL,Combes V,Grau GE

11-0699 was used in Flow cytometry/Cell sorting to explore the effect of endothelial cell microparticles in T cell activation and antigen presentation.

Wed Oct 01 00:00:00 EDT 2014

Invariant natural killer T cells act as an extravascular cytotoxic barrier for joint-invading Lyme Borrelia.

Proceedings of the National Academy of Sciences of the United States of America

Lee WY,Sanz MJ,Wong CH,Hardy PO,Salman-Dilgimen A,Moriarty TJ,Chaconas G,Marques A,Krawetz R,Mody CH,Kubes P

11-0699 was used in Flow cytometry/Cell sorting to demonstrate a novel, critical extravascular invariant natural killer T cell-mediated mechanism of immune surveillance in joints.

Tue Sep 23 00:00:00 EDT 2014

Equisetum arvense (common horsetail) modulates the function of inflammatory immunocompetent cells.

BMC complementary and alternative medicine

Gründemann C,Lengen K,Sauer B,Garcia-Käufer M,Zehl M,Huber R

11-0699 was used in Flow cytometry/Cell sorting to understand the molecular basis for the use of common horsetail in the treatment of inflammatory disorders.

Mon Aug 04 00:00:00 EDT 2014

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD69 monoclonal antibody (Product # 11-0699-42) in Flow Cytometry

Thu Apr 10 00:00:00 EDT 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD69 monoclonal antibody (Product # 11-0699-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

11-0699 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

Gene Aliases: AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3

UniProt ID: (Human) Q07108

Entrez Gene ID: (Human) 969

Function(s)

signal transduction cellular response to drug

Process(es)

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding