Invitrogen

CD69 Monoclonal Antibody (FN50), APC, eBioscience™

Product Image Gallery

CD69 Antibody in Flow Cytometry (Flow) — Staining of overnight PHA-stimulated normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0037-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD69 APC (right). Cells in the lymphocyte gate were used for analysis.

Product Details

17-0699-42

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	5 µL (0.015 µg)/test	View 13 publications 13 publications

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	FN50
Conjugate	APC Alexa Fluor 561 Alexa Fluor 660 APC-eFluor 780 Biotin Brilliant UV 737 Brilliant UV 805 eFluor 450 FITC PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 436 Super Bright 600 Super Bright 780 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2016681

Product Specific Information

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.

Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

T cell receptor-dependent S-acylation of ZAP-70 controls activation of T cells.

The Journal of biological chemistry

Tewari R,Shayahati B,Fan Y,Akimzhanov AM

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Thu Aug 26 00:00:00 EDT 2021

Glossogyne tenuifolia (Hsiang-ju) extract suppresses T cell activation by inhibiting activation of c-Jun N-terminal kinase.

Chinese medicine

Houng JY,Tai TS,Hsu SC,Hsu HF,Hwang TS,Lin CJ,Fang LW

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Heterogeneity of human bone marrow and blood natural killer cells defined by single-cell transcriptome.

Nature communications

Yang C,Siebert JR,Burns R,Gerbec ZJ,Bonacci B,Rymaszewski A,Rau M,Riese MJ,Rao S,Carlson KS,Routes JM,Verbsky JW,Thakar MS,Malarkannan S

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Mon Sep 02 00:00:00 EDT 2019

Combinations of isoform-targeted histone deacetylase inhibitors and bryostatin analogues display remarkable potency to activate latent HIV without global T-cell activation.

Scientific reports

Albert BJ,Niu A,Ramani R,Marshall GR,Wender PA,Williams RM,Ratner L,Barnes AB,Kyei GB

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells.

eLife

Reeder JE,Kwak YT,McNamara RP,Forst CV,D'Orso I

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2015

Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system.

mAbs

Xu Y,Lee J,Tran C,Heibeck TH,Wang WD,Yang J,Stafford RL,Steiner AR,Sato AK,Hallam TJ,Yin G

17-0699 was used in Flow cytometry/Cell sorting to develop a cell-free expression system to produce knobs-into-holes bispecific antibodies in multiple scaffolds.

Mon Sep 28 00:00:00 EDT 2015

Biallelic loss-of-function mutation in NIK causes a primary immunodeficiency with multifaceted aberrant lymphoid immunity.

Nature communications

Willmann KL,Klaver S,Doğu F,Santos-Valente E,Garncarz W,Bilic I,Mace E,Salzer E,Conde CD,Sic H,Májek P,Banerjee PP,Vladimer GI,Haskoloğlu S,Bolkent MG,Küpesiz A,Condino-Neto A,Colinge J,Superti-Furga G,Pickl WF,van Zelm MC,Eibel H,Orange JS,Ikincioğulları A,Boztuğ K

17-0699 was used in Flow cytometry/Cell sorting to study the effect of a biallelic mutation in the gene encoding NF-κB-inducing kinase on immune cell populations.

Wed Nov 19 00:00:00 EST 2014

Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function.

Molecular cell

Guo YE,Riley KJ,Iwasaki A,Steitz JA

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Thu Apr 10 00:00:00 EDT 2014

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD69 monoclonal antibody (Product # 17-0699-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Monocytes regulate the mechanism of T-cell death by inducing Fas-mediated apoptosis during bacterial infection.

PLoS pathogens

Daigneault M,De Silva TI,Bewley MA,Preston JA,Marriott HM,Mitchell AM,Mitchell TJ,Read RC,Whyte MK,Dockrell DH

17-0699 was used in Flow cytometry/Cell sorting to elucidate the mechanisms that regulate levels of activated T-cells at sites of acute bacterial infection.

Tue Jan 29 00:00:00 EST 2013

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

17-0699 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: Activation inducer molecule; activation inducer molecule (AIM/CD69); AIM; BL-AC/P26; C-type lectin domain family 2 member C; C-type lectin domain family 2, member C; CD69; CD69 antigen (p60, early T-cell activation antigen); EA1; Early activation antigen CD69; early lymphocyte activation antigen; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3

Gene Aliases: AIM; BL-AC/P26; CD69; CLEC2C; EA1; GP32/28; MLR-3

UniProt ID: (Human) Q07108

Entrez Gene ID: (Human) 969

Function(s)

signal transduction cellular response to drug

Process(es)

transmembrane signaling receptor activity calcium ion binding protein binding carbohydrate binding