Invitrogen

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Super Bright™ 436, eBioscience™

CD90 (Thy-1) Antibody in Flow Cytometry (Flow)

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CD90 (Thy-1) Antibody (62-0909-42) in Flow

Staining of HEL cell line with Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (blue histogram) or Anti-Human CD90 (Thy-1) Super Bright 436 (purple histogram). Total viable cells were used for analysis.

Product Image Gallery

CD90 (Thy-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig. 5. THY1 expression in epicardium. (A) Immunofluorescence double labelling for THY1 and WT1 in hPSC-epi. (B,C) Immunofluorescence double labelling in an epicardial explant from an 8 week pc human embryo for THY1 and WT1 (B) or THY1 and BNC1 (C). (D) THY1 immunofluorescence staining on a cryosection of a human heart at 8 weeks pc. The regions of high or low expression of THY1 are indicated by arrows or arrowheads, respectively. Scale bars: 40 mum (A); 20 mum (B,C); 50 mum (D).

Product Details

62-0909-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio5E10 (5E10)

Conjugate

Super Bright™ 436

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2637318

Product Specific Information

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of the HEL cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Fluorescence-activated cell sorting and phenotypic characterization of human fibro-adipogenic progenitors.

STAR protocols

Billeskov TB,Jensen JB,Jessen N,Farup J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Jan 12 00:00:00 EST 2023

Aberrant Expression of COX-2 and FOXG1 in Infrapatellar Fat Pad-Derived ASCs from Pre-Diabetic Donors.

Cells

O'Donnell BT,Monjure TA,Al-Ghadban S,Ives CJ,L'Ecuyer MP,Rhee C,Romero-Lopez M,Li Z,Goodman SB,Lin H,Tuan RS,Bunnell BA

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246.

Stem cell research & therapy

Liu P,Zhang Q,Mi J,Wang S,Xu Q,Zhuang D,Chen W,Liu C,Zhang L,Guo J,Wu X

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Mar 03 00:00:00 EST 2022

Fused Cells between Human-Adipose-Derived Mesenchymal Stem Cells and Monocytes Keep Stemness Properties and Acquire High Mobility.

International journal of molecular sciences

Montalbán-Hernández K,Casado-Sánchez C,Avendaño-Ortiz J,Casalvilla-Dueñas JC,Bonel-Pérez GC,Prado-Montero J,Valentín-Quiroga J,Lozano-Rodríguez R,Terrón-Arcos V,de la Bastida FR,Córdoba L,Laso-García F,Diekhorst L,Del Fresno C,López-Collazo E

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Fri Aug 26 00:00:00 EDT 2022

Cell-mimicking nanodecoys neutralize SARS-CoV-2 and mitigate lung injury in a non-human primate model of COVID-19.

Nature nanotechnology

Li Z,Wang Z,Dinh PC,Zhu D,Popowski KD,Lutz H,Hu S,Lewis MG,Cook A,Andersen H,Greenhouse J,Pessaint L,Lobo LJ,Cheng K

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Exosomes Regulate Interclonal Communication on Osteogenic Differentiation Among Heterogeneous Osteogenic Single-Cell Clones Through PINK1/Parkin-Mediated Mitophagy.

Frontiers in cell and developmental biology

Fei D,Xia Y,Zhai Q,Wang Y,Zhou F,Zhao W,He X,Wang Q,Jin Y,Li B

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Oct 05 00:00:00 EDT 2021

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching.

Scientific reports

Chen C,Huang X,Zhu W,Ding C,Huang P,Li R

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Aug 31 00:00:00 EDT 2021

In Vitro Anti-cancer Activity of Adipose-Derived Mesenchymal Stem Cells Increased after Infection with Oncolytic Reovirus.

Advanced pharmaceutical bulletin

Babaei A,Bannazadeh Baghi H,Nezhadi A,Jamalpoor Z

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model.

Stem cell research & therapy

Li Y,Shi G,Han Y,Shang H,Li H,Liang W,Zhao W,Bai L,Qin C

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Jul 15 00:00:00 EDT 2021

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.

Development (Cambridge, England)

Gambardella L,McManus SA,Moignard V,Sebukhan D,Delaune A,Andrews S,Bernard WG,Morrison MA,Riley PR,Göttgens B,Gambardella Le Novère N,Sinha S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Immunocytochemistry

Fri Dec 13 00:00:00 EST 2019

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.

Development (Cambridge, England)

Gambardella L,McManus SA,Moignard V,Sebukhan D,Delaune A,Andrews S,Bernard WG,Morrison MA,Riley PR,Göttgens B,Gambardella Le Novère N,Sinha S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Immunocytochemistry

Fri Dec 13 00:00:00 EST 2019

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium.

Development (Cambridge, England)

Gambardella L,McManus SA,Moignard V,Sebukhan D,Delaune A,Andrews S,Bernard WG,Morrison MA,Riley PR,Göttgens B,Gambardella Le Novère N,Sinha S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Immunocytochemistry

Fri Dec 13 00:00:00 EST 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer.

Cancers

Huang JY,Wang YY,Lo S,Tseng LM,Chen DR,Wu YC,Hou MF,Yuan SF

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Fri Dec 20 00:00:00 EST 2019

Spatial and Single-Cell Transcriptional Profiling Identifies Functionally Distinct Human Dermal Fibroblast Subpopulations.

The Journal of investigative dermatology

Philippeos C,Telerman SB,Oulès B,Pisco AO,Shaw TJ,Elgueta R,Lombardi G,Driskell RR,Soldin M,Lynch MD,Watt FM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

CD90 promotes cell migration, viability and sphere‑forming ability of hepatocellular carcinoma cells.

International journal of molecular medicine

Zhang K,Che S,Su Z,Zheng S,Zhang H,Yang S,Li W,Liu J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Comprehensive characterization of chorionic villi-derived mesenchymal stromal cells from human placenta.

Stem cell research & therapy

Ventura Ferreira MS,Bienert M,Müller K,Rath B,Goecke T,Opländer C,Braunschweig T,Mela P,Brümmendorf TH,Beier F,Neuss S

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Mon Feb 05 00:00:00 EST 2018

Endothelial and smooth muscle cells derived from human cardiac explants demonstrate angiogenic potential and suitable for design of cell-containing vascular grafts.

Journal of translational medicine

Zakharova IS,Zhiven' MK,Saaya SB,Shevchenko AI,Smirnova AM,Strunov A,Karpenko AA,Pokushalov EA,Ivanova LN,Makarevich PI,Parfyonova YV,Aboian E,Zakian SM

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.

American journal of physiology. Gastrointestinal and liver physiology

Gargus M,Niu C,Vallone JG,Binkley J,Rubin DC,Shaker A

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Immunophenotypic comparison of heterogenous non-sorted versus sorted mononuclear cells from human umbilical cord blood: a novel cell enrichment approach.

Cytotechnology

Indumathi S,Harikrishnan R,Rajkumar JS,Dhanasekaran M

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Thu Dec 11 00:00:00 EST 2014

PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.

International journal of clinical and experimental pathology

Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN

Published figure using CD90 (Thy-1) monoclonal antibody (Product # 62-0909-42) in Flow Cytometry

Tue May 26 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD90; CDw90; FLJ33325; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy-1 T-cell antigen

Gene Aliases: CD90; CDw90; THY1

UniProt ID: (Human) P04216

Entrez Gene ID: (Human) 7070

Molecular Function: cell adhesion molecule

Function(s)

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

Process(es)

GTPase activator activity integrin binding protein binding protein kinase binding GPI anchor binding

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Super Bright™ 436, eBioscience™

CD90 (Thy-1) Antibody (62-0909-42) in Flow

Product Details

62-0909-42

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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