Invitrogen

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), Super Bright™ 436, eBioscience™

Product Image Gallery

CD90 (Thy-1) Antibody in Flow Cytometry (Flow) — Staining of HEL cell line with Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (blue histogram) or Anti-Human CD90 (Thy-1) Super Bright 436 (purple histogram). Total viable cells were used for analysis.

CD90 (Thy-1) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Fig. 5. THY1 expression in epicardium. (A) Immunofluorescence double labelling for THY1 and WT1 in hPSC-epi. (B,C) Immunofluorescence double labelling in an epicardial explant from an 8 week pc human embryo for THY1 and WT1 (B) or THY1 and BNC1 (C). (D) THY1 immunofluorescence staining on a cryosection of a human heart at 8 weeks pc. The regions of high or low expression of THY1 are indicated by arrows or arrowheads, respectively. Scale bars: 40 mum (A); 20 mum (B,C); 50 mum (D).

Product Details

62-0909-42

Applications	Tested Dilution	Publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	5 µL (0.25 µg)/test	View 19 publications 19 publications

Product Specifications
Species Reactivity	Human
Published species	Not Applicable
Host/Isotype	Mouse / IgG1, kappa
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 436, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	eBio5E10 (5E10)
Conjugate	Super Bright™ 436 Alexa Fluor 700 APC APC-eFluor 780 Biotin FITC PE PE-Cyanine5 PE-Cyanine7 PerCP-Cyanine5.5 PerCP-eFluor 710 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	5 µL/Test
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2637318

Product Specific Information

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kDa receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5E10 (5E10) antibody has been pre-titrated and tested by flow cytometric analysis of the HEL cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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