|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (535 RPRGQPPVPPKPSIR 549) of CENTB1/ACAP1 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1.0-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ACAP1, also known as Centaurin-beta-1 (CENTB1), is a 740 amino acid containing transport carrier belonging to the Centaurin family with three ANK repeats, an Arf-GAP domain, a BAR domain and a PH domain that binds to phospholipids. ACAP1, a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6), is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. It mediates CENTB1-binding to phosphatidylinositol 3,5-bisphosphate (PIP2) or phosphatidylinositol 3,4,5-trisphosphate (PIP3) containing membranes. Along with ACAP2, ACAP1 is recruited to platelet-derived growth factor (PDGF) induced dorsal membrane ruffles in NIH 3T3 mouse fibroblasts, while over expression inhibited ruffle formation. It is widely expressed in most tissues with high expression in lung and spleen and low expression in heart, kidney, liver and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.