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CFI Antibody (CF806033) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CFI (RC216645, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CFI. Positive lysates LY424865 (100 µg) and LC424865 (20 µg) can be purchased separately from OriGene.
Product Details
CF806033
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI15E11
Immunogen
Human recombinant protein fragment corresponding to amino acids 340-583 of human CFI produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
Factor I, a serine protease, is a regulatory component of both the alternative and the classical complement pathway. Factor I is synthesized as a single chain precursor of 88 kDa, processed to yield the active form, composed of two disulfide-linked chains, the a-chain (50 kDa) and the b-chain (38 kDa). Plasma concentration is 35 µg/mL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C3b-inactivator; C3B/C4B inactivator; complement component I; complement control protein factor I; Complement factor I; complement factor I heavy chain; factor i; Konglutinogen-activating factor; light chain of factor I
Gene Aliases: AHUS3; ARMD13; C3b-INA; C3BINA; CFI; FI; IF; KAF
UniProt ID: (Human) P05156
Entrez Gene ID: (Human) 3426
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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