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CRTAC1 Antibody (MA5-24362) in ICC/IF
Immunocytochemistry analysis of CRTAC1 in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in CRTAC1 monoclonal antibody (Product # MA5-24362) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
Product Details
MA5-24362
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
755315
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRTAC1 Ser28-Cys661
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2577084
Product Specific Information
Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
CRTAC1 encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 68 kDa chondrocyte-expressed protein; acidic secreted protein in cartilage; ASPIC; Cartilage acidic protein 1; CEP-68; chondrocyte expressed protein 68 kDa CEP-68; FLJ10320
Gene Aliases: ASPIC; ASPIC1; CEP-68; CEP68; CRTAC1
UniProt ID: (Human) Q9NQ79
Entrez Gene ID: (Human) 55118
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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