OriGene

CSNK1E Monoclonal Antibody (OTI5C9), TrueMAB™

Product Image Gallery

CSNK1E Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSNK1E (RC202436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-CSNK1. Positive lysates LY403455 (100 µg) and LC403455 (20 µg) can be purchased separately from OriGene.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5C9

Immunogen

Human recombinant protein fragment corresponding to amino acids 153-416 of human CSNK1E produced in E.coli.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

Casein kinases are a group of ubiquitous Ser/Thr kinases that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation, circadian rhythms, and metabolic pathways. CK1 family members share a highly conserved kinase domain but differ in their variable N-and C-terminal domains. Mammals have seven family members: alpha, beta, gamma1, gamma2, gamma3, delta and epsilon. They all share at least 50% amino acid identity within the protein kinase catalytic domain. CK1 isoforms exclusively use ATP as phosphate donor and are generally co-factor independent. Casein kinase I epsilon is a Wnt-regulated kinase, and regulated phosphorylation of Dvl allows fine tuning of the Wnt-b-catenin signaling pathway. CKIe positively regulates Akt possibly by inhibiting PP2A. CKIe play an important role in neurodegenerative diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: casein kinase 1; casein kinase 1, epsilon; Casein kinase I isoform epsilon; CK1e; CKI-epsilon; CKIe; MGC10398; OTTHUMP00000063258; OTTHUMP00000063263; RP1-5O6.1

Gene Aliases: CKIepsilon; CSNK1E; HCKIE

UniProt ID: (Human) P49674

Entrez Gene ID: (Human) 1454

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase