Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis was performed on tissue extracts (30 ug lysate) of Mouse Testis (Lane 1), and Rat Testis (Lane 2). The blots were probed with Anti- CUL-5/VACM-1 Mouse Monoclonal Antibody (Product# 399000 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). A ~ 63 kDa band corresponding to CUL-5/VACM-1 was observed across cell lines tested. Additionally ~25 kDa band was observed in mouse testis. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide derived from the N-terminal region of the mouse and rat CUL-5/VACM-1 proteins, which are 89% homologous to human|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||1-2 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. Seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CUL-5; cullin-5; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); VACM-1; VACM1; vasopressin-activated calcium-mobilizing receptor 1; vasopressin-activated calcium-mobilizing receptor protein; Vasopressin-activated calcium-mobilizing receptor-1
4921514I20Rik; 8430423K24Rik; AI852817; C030032G03Rik; C330021I08Rik; CUL5; VACM-1; VACM1