Invitrogen

CXCL16 Monoclonal Antibody (GT516)

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CXCL16 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — CXCL16 Monoclonal Antibody (GT516) detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

CXCL16 Antibody in Western Blot (WB) — Western Blot analysis of CXCL16 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) at a dilution of 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

CXCL16 Antibody in Immunohistochemistry (IHC) — Figure 3. CD11c + cells express CXCL16 in melanoma-associated vitiligo (MAV)-affected skin. (A) Heat map showing gene expression of chemokines and receptors in CD11c + CD11b neg , CD11c + CD11b + , and CD8 + T cells sorted from skin of MAV-affected mice. Data presented as log2-normalized expression. (B) Surface expression of CXCL16 on CD11c + cells isolated from skin of naive (n = 3) or MAV-affected (n = 5) mice, skin-draining LNs, or spleen 7, 14, and 35 d after surgery. Representative of three independent experiments. (C) Surface expression of CXCL16 on CD45 + CD11c + , CD45 + CD11c neg , and CD45 neg cells isolated from skin of MAV-affected mice, 35 d after surgery. Representative of two independent experiments with skin collected between 35- and 60-d post-surgery. Dotted horizontal line indicates background CXCL16 expression in unstained cell sample. (D) Expression of CXCL16 and CD11c in unaffected and MAV-affected mice; CD11c (green), CXCL16 (red), and nuclei (blue). Scale bar, 50 mum. (E) CXCL16 expression in IF images from skin of unaffected and MAV-affected mice; n = 5-8 mice. (F) CXCL16 expression in hair follicles with or without CD11c + cell clusters in MAV-affected skin; n = 8 mice. (G) CD11c + and CXCL16 + cells in skin from MAV-affected patients. Stains identify CXCL16 (green) and CD11c (red); dark red identifies colocalization of CD11c and CXCL16. Scale bar, 25 mum. (H) Percent of CD11c + cells expressing CXCL16 in MAV-affected patient skin. (B, C, H) Symbols r

Product Details

MA5-27845

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:10,000	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Rat
Published species	Not Applicable
Host/Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Clone	GT516
Immunogen	Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	no preservative
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735159

Product Specific Information

Positive Control: HeLa, HeLa membrane extract

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CXCL16 acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells.

Life science alliance

Vella JL,Molodtsov A,Angeles CV,Branchini BR,Turk MJ,Huang YH

Published figure using CXCL16 monoclonal antibody (Product # MA5-27845) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 16; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; CXC chemokine ligand 16; CXC motif chemokine; CXCL; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16; SR-PSOX; Transmembrane chemokine CXCL16

Gene Aliases: CXCL16; CXCLG16; SCYB16; SR-PSOX; SRPSOX; UNQ2759/PRO6714

UniProt ID: (Human) Q9H2A7, (Rat) Q6AXU5

Entrez Gene ID: (Human) 58191, (Rat) 497942

Molecular Function: chemokine cytokine intercellular signal molecule