Invitrogen

CXCL16 Monoclonal Antibody (GT516)

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Datasheet

Protocols

Questions & Answers

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Antibody Testing Data (3)
Published Figures (1)

CXCL16 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 4

CXCL16 Antibody (MA5-27845) in IHC (P)

CXCL16 Monoclonal Antibody (GT516) detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis. Sample: Paraffin-embedded rat lymph node. CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

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CXCL16 Antibody in Western Blot (WB) — Western Blot analysis of CXCL16 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CXCL16 Monoclonal Antibody (GT516) (Product # MA5-27845) at a dilution of 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

CXCL16 Antibody in Immunohistochemistry (IHC) — Figure 3. CD11c + cells express CXCL16 in melanoma-associated vitiligo (MAV)-affected skin. (A) Heat map showing gene expression of chemokines and receptors in CD11c + CD11b neg , CD11c + CD11b + , and CD8 + T cells sorted from skin of MAV-affected mice. Data presented as log2-normalized expression. (B) Surface expression of CXCL16 on CD11c + cells isolated from skin of naive (n = 3) or MAV-affected (n = 5) mice, skin-draining LNs, or spleen 7, 14, and 35 d after surgery. Representative of three independent experiments. (C) Surface expression of CXCL16 on CD45 + CD11c + , CD45 + CD11c neg , and CD45 neg cells isolated from skin of MAV-affected mice, 35 d after surgery. Representative of two independent experiments with skin collected between 35- and 60-d post-surgery. Dotted horizontal line indicates background CXCL16 expression in unstained cell sample. (D) Expression of CXCL16 and CD11c in unaffected and MAV-affected mice; CD11c (green), CXCL16 (red), and nuclei (blue). Scale bar, 50 mum. (E) CXCL16 expression in IF images from skin of unaffected and MAV-affected mice; n = 5-8 mice. (F) CXCL16 expression in hair follicles with or without CD11c + cell clusters in MAV-affected skin; n = 8 mice. (G) CD11c + and CXCL16 + cells in skin from MAV-affected patients. Stains identify CXCL16 (green) and CD11c (red); dark red identifies colocalization of CD11c and CXCL16. Scale bar, 25 mum. (H) Percent of CD11c + cells expressing CXCL16 in MAV-affected patient skin. (B, C, H) Symbols r

Product Details

MA5-27845

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT516

Immunogen

Recombinant protein encompassing a sequence within the center region of human CXCL16. The exact sequence is proprietary.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2735159

Product Specific Information

Positive Control: HeLa, HeLa membrane extract

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cerebrospinal fluid immune dysregulation during healthy brain aging and cognitive impairment.

Cell

Piehl N,van Olst L,Ramakrishnan A,Teregulova V,Simonton B,Zhang Z,Tapp E,Channappa D,Oh H,Losada PM,Rutledge J,Trelle AN,Mormino EC,Elahi F,Galasko DR,Henderson VW,Wagner AD,Wyss-Coray T,Gate D

MA5-27845 was used in Immunohistochemistry to reveal cerebrospinal fluid immune dysregulation during healthy brain aging and cognitive impairment.

Thu Dec 22 00:00:00 EST 2022

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells.

Life science alliance

Vella JL,Molodtsov A,Angeles CV,Branchini BR,Turk MJ,Huang YH

Published figure using CXCL16 monoclonal antibody (Product # MA5-27845) in Immunohistochemistry

Fri Oct 01 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 16; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; CXC chemokine ligand 16; CXC motif chemokine; CXCL; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16; SR-PSOX; Transmembrane chemokine CXCL16

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Gene Aliases: CXCL16; CXCLG16; SCYB16; SR-PSOX; SRPSOX; UNQ2759/PRO6714

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UniProt ID: (Human) Q9H2A7, (Rat) Q6AXU5

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Entrez Gene ID: (Human) 58191, (Rat) 497942

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Molecular Function: chemokine cytokine intercellular signal molecule

Function(s)

receptor-mediated endocytosis chemotaxis T cell chemotaxis positive regulation of cell growth positive regulation of cell migration response to cytokine response to interferon-gamma response to tumor necrosis factor cellular response to lipopolysaccharide

Process(es)

low-density lipoprotein receptor activity scavenger receptor activity chemokine activity chemokine receptor binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL16 Monoclonal Antibody (GT516)

CXCL16 Antibody (MA5-27845) in IHC (P)

Product Details

MA5-27845

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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