Antibody binding specificity was determined by Particle Concentration Fluorescence Immunoassay (PCFIA) using the yeast caboxypeptidase, by Western blot immunoassay using protein extracts from yeast, and by indirect immunofluorescence of fixed yeast cells.The antibody can be used to detect the yeast carboxypeptidase on Western blots or to immunolocalize the carboxypeptidase in fixed yeast cells. The antibody is ideal for screening for caroboxypeptidase secretion (Vps-phenotype) in yeast using the colony immunoblot overlay assay. The antibody has been extensively used in screens for new vps mutations, as well as in complementation analysis with the vps mutant collection.
Carboxypeptidase Y is Involved in the degradation of small peptides. It digests peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in the P1 position of an ester substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: carboxypeptidase C PRC1; Carboxypeptidase Y; Carboxypeptidase YSCY; CPD-Y; cpy1; pcy1
Gene Aliases: CPY1; LBC1; PRC1; YMR297W
UniProt ID: (Baker's yeast) P00729
Entrez Gene ID: (Baker's yeast) 855343