Immunofluorescent analysis of Cell cycle-related kinase3 showing staining in the cytoplasm and nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Cell cycle-related kinase3 polyclonal antibody (Product # PA5-28208) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 7 and 319 of Human Cell cycle-related kinase3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28208 targets Cell cycle-related kinase3 in WB applications and shows reactivity with Human samples.
The PA5-28208 immunogen is recombinant fragment corresponding to a region within amino acids 7 and 319 of Human Cell cycle-related kinase3.
The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase activates CDK2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
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