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Immunofluorescent analysis of Cell cycle-related kinase3 showing staining in the cytoplasm and nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Cell cycle-related kinase3 polyclonal antibody (Product # PA5-28208) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 7 and 319 of Human Cell cycle-related kinase3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28208 targets Cell cycle-related kinase3 in WB applications and shows reactivity with Human samples.
The PA5-28208 immunogen is recombinant fragment corresponding to a region within amino acids 7 and 319 of Human Cell cycle-related kinase3.
The protein encoded by this gene contains a kinase domain most closely related to the cyclin-dependent protein kinases. The encoded kinase activates CDK2 and is involved in cell growth. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CAK-kinase p42; CCRK; CDCH; CDK-activating kinase p42; cell cycle-related kinase; cell division protein kinase 20; cyclin-dependent protein kinase H; cyclin-kinase-activating kinase p42; PNQALRE
CCRK; CDCH; CDK20; P42; PNQALRE