Invitrogen

Cullin 4A Recombinant Rabbit Monoclonal Antibody (JU07-33)

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Questions & Answers

Questions & Answers

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Cullin 4A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Cullin 4a in HUVEC cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).

Cullin 4A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Cullin 4a in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), followed by DAPI (blue).

Cullin 4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human breast cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.

Cullin 4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human liver cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.

Cullin 4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human colon cancer tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.

Cullin 4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human kidney tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.

Cullin 4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Cullin 4a in paraffin-embedded human cervix tissue. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), and followed by hematoxylin.

Cullin 4A Antibody in Western Blot (WB) — Western blot analysis of Cullin 4a in HeLa cell lysates. Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615), at a dilution of 1:500.

Cullin 4A Antibody in Flow Cytometry (Flow) — Flow cytometry of Cullin 4a in HeLa cells (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Samples were incubated with Cullin 4a monoclonal antibody (Product # MA5-34615) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

MA5-34615

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JU07-33
Immunogen	Synthetic peptide within Human Cullin 4a aa 710-759 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848523

Product Specific Information

Positive Control: Hela, A431, HUVEC, human liver cance tissue, human breast cancer tissue, human colon cancer tissue, human kidney tissue, human cervix tissue.

Target Information

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CUL 4A; CUL-4A; Cul4a protein; Cullin-4A; MGC36573; MGC64071; OTTHUMP00000018753

Gene Aliases: CUL4A

UniProt ID: (Human) Q13619

Entrez Gene ID: (Human) 8451

Molecular Function: ubiquitin-protein ligase

G1/S transition of mitotic cell cycle nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding in utero embryonic development transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion cellular response to DNA damage stimulus cell cycle arrest positive regulation of cell proliferation negative regulation of cell proliferation viral process hemopoiesis negative regulation of granulocyte differentiation nucleotide-excision repair, DNA incision somatic stem cell population maintenance DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process regulation of protein metabolic process global genome nucleotide-excision repair intrinsic apoptotic signaling pathway positive regulation of G1/S transition of mitotic cell cycle regulation of DNA damage checkpoint regulation of nucleotide-excision repair

Process(es)

protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity