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Invitrogen
Cytokeratin 20 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
Cytokeratin 20 Antibody (PA5-22125) in WB
Western blot was performed using Anti-Cytokeratin 20 Polyclonal Antibody (Product # PA5-22125) on whole cell extracts (30 µg lysate) of HT-29 (Lane 1), HCT-116 (Lane 2) and MCF7 (Lane 3) and 48.48 kDa band corresponding to Cytokeratin 20 was observed only in HT-29 which is reported to be the positive model. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by Goat anti-Ra... View More
Product Details
PA5-22125
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 75 and 317 of Cytokeratin 20 (Uniprot ID#P35900)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.88 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_11154993
Product Specific Information
Recommended positive controls: HeLa.
Predicted reactivity: Mouse (82%), Rat (83%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-20; cytokeratin 20; Cytokeratin-20; K20; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; krt20; MGC35423; Protein IT
Gene Aliases: 9030623C06Rik; CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900, (Mouse) Q9D312
Entrez Gene ID: (Human) 54474, (Mouse) 66809
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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