Invitrogen

Cytokeratin 8 Monoclonal Antibody (TS1)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure

14 References

View all (99) Cytokeratin 8 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Cytokeratin 8 Antibody in Western Blot (WB)

FIGURE: 1 / 10

Cytokeratin 8 Antibody (MA5-14428) in WB

Western blot was performed using Anti-Cytokeratin 8 Monoclonal Antibody (TS1) (Product # MA5-14428) and a 53 kDa band corresponding to KRT8 was observed across the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), A-431 (Lane 2), MCF7 (Lane 3), HaCaT (Lane 4) and Caco-2 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was p... View More

Product Image Gallery

Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Cytokeratin 8 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 8 (TS1) Mouse Monoclonal Antibody (Product # MA5-14428) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Cytokeratin 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human breast carcinoma stained with Keratin 8 antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.

Cytokeratin 8 Antibody in Immunohistochemistry (IHC) — Figure 4. Staining of imaging-positive lymph nodes. H&E staining of imaging-positive lymph nodes at (A) x100 and (B) x400 magnification. IHC analysis of human CK8 in imaging-positive lymph nodes at (C) x100 and (D) x400 magnification. IHC analysis of human CK7 in imaging-positive lymph nodes at (E) x100 and (F) x400 magnification. Positive staining of tumor cells was sporadically distributed. H&E, hematoxylin and eosin; IHC, immunohistochemistry; CK, cytokeratin.

Cytokeratin 8 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in Cytokeratin 8 KO cell line compared to control cell line using Cytokeratin 8 Monoclonal Antibody (TS1) (Product # MA5-14428). {KO}

Product Details

MA5-14428

Applications

Tested Dilution

Publications

Western Blot (WB)

1:25-1:1,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 3 publications 3 publications

Immunoprecipitation (IP)

1:50

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

TS1

Immunogen

Keratin preparation from a human carcinoma

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10986025

Product Specific Information

MA5-14428 targets Cytokeratin 8 in IHC (P) and IP applications and shows reactivity with Human samples.

The MA5-14428 immunogen is keratin preparation from a human carcinoma.

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pituispheres Contain Genetic Variants Characteristic to Pituitary Adenoma Tumor Tissue.

Frontiers in endocrinology

Peculis R,Mandrika I,Petrovska R,Dortane R,Megnis K,Nazarovs J,Balcere I,Stukens J,Konrade I,Pirags V,Klovins J,Rovite V

MA5-14428 was used in Immunohistochemistry to trace the origin of sphere-forming and adherent pituitary cell cultures and characterize the potential use of these surgery derived cell lines as pituitary adenoma model.

Mon May 10 00:00:00 EDT 2021

Delineation of retroperitoneal metastatic lymph nodes in ovarian cancer with near-infrared fluorescence imaging.

Oncology letters

Pu T,Xiong L,Liu Q,Zhang M,Cai Q,Liu H,Sood AK,Li G,Kang Y,Xu C

Published figure using Cytokeratin 8 monoclonal antibody (Product # MA5-14428) in Immunohistochemistry

Fri Sep 01 00:00:00 EDT 2017

FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes.

Scientific reports

Kuga T,Sasaki M,Mikami T,Miake Y,Adachi J,Shimizu M,Saito Y,Koura M,Takeda Y,Matsuda J,Tomonaga T,Nakayama Y

MA514428 was used in immunocytochemistry and western blot to examine the effects of the FAM83H mutation on the keratin cytoskeleton and desmosomes in ameloblasts

Wed May 25 00:00:00 EDT 2016

Casein kinase 1 is recruited to nuclear speckles by FAM83H and SON.

Scientific reports

Kuga T,Kume H,Adachi J,Kawasaki N,Shimizu M,Hoshino I,Matsubara H,Saito Y,Nakayama Y,Tomonaga T

MA514428 was used in western blot to study factors that influence the localization of casein kinase 1

Thu Sep 29 00:00:00 EDT 2016

Ambient Light Promotes Selective Subcellular Proteotoxicity after Endogenous and Exogenous Porphyrinogenic Stress.

The Journal of biological chemistry

Maitra D,Elenbaas JS,Whitesall SE,Basrur V,D'Alecy LG,Omary MB

MA5-14428 was used in Western Blotting to study whether extracellular or intracellular protoporphyria cause damage to different subcellular compartments, in a light-triggered manner.

Fri Sep 25 00:00:00 EDT 2015

Multiple oncocytic cystadenoma with intraluminal crystalloids in parotid gland: case report.

Medicine

Başak K,Kroğlu K

MA5-14428 was used in immunohistochemistry to describe a tumor from a patient with oncocytic cystadenoma

Mon Dec 01 00:00:00 EST 2014

Glucose and SIRT2 reciprocally mediate the regulation of keratin 8 by lysine acetylation.

The Journal of cell biology

Snider NT,Leonard JM,Kwan R,Griggs NW,Rui L,Omary MB

MA5-14428 was used in immunoprecipitation to study the opposing effects of hyperglycemia and SIRT2 on keratin-8 lysine acetylation

Mon Feb 04 00:00:00 EST 2013

Aging modulates susceptibility to mouse liver Mallory-Denk body formation.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Hanada S,Harada M,Abe M,Akiba J,Sakata M,Kwan R,Taniguchi E,Kawaguchi T,Koga H,Nagata E,Ueno T,Sata M

MA5-14428 was used in western blot to study the effect of aging on Mallory-Denk body formation in mouse liver

Fri Jun 01 00:00:00 EDT 2012

Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch.

The Journal of biological chemistry

Duan Y,Sun Y,Zhang F,Zhang WK,Wang D,Wang Y,Cao X,Hu W,Xie C,Cuppoletti J,Magin TM,Wang H,Wu Z,Li N,Huang P

MA5-14428 was used in western blot to study the effect of keratin 18 overexpression on the cell surface expression of the cystic fibrosis transmembrane conductance regulator

Fri Nov 23 00:00:00 EST 2012

Molecular characterization of breast cancer in young Brazilian women.

Revista da Associacao Medica Brasileira (1992)

Carvalho LV,Pereira EM,Frappart L,Boniol M,Bernardo WM,Tarricone V,Tavtigian S,Southey MC

MA5-14428 was used in immunohistochemistry to study the molecular features of breast cancer in young Brazilian women

Fri Aug 26 00:00:00 EDT 2011

Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.

The Journal of biological chemistry

Snider NT,Weerasinghe SV,Iñiguez-Lluhí JA,Herrmann H,Omary MB

MA5-14428 was used in immunocytochemistry to study keratin hypersumoylation and its utility as a marker of human liver disease and mutation of keratin

Fri Jan 21 00:00:00 EST 2011

Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.

Toxicological sciences : an official journal of the Society of Toxicology

Page JL,Johnson MC,Olsavsky KM,Strom SC,Zarbl H,Omiecinski CJ

MA5-14428 was used in western blot to examine the biological responses imparted by Matrigel overlays on primary human hepatocyte cultures

Fri Jun 01 00:00:00 EDT 2007

Keratins modulate c-Flip/extracellular signal-regulated kinase 1 and 2 antiapoptotic signaling in simple epithelial cells.

Molecular and cellular biology

Gilbert S,Loranger A,Marceau N

MA5-14428 was used in western blot to study the role of keratins in modulating cFlip and ERK1/2 apoptotic signaling in epithelial cells

Sun Aug 01 00:00:00 EDT 2004

Functional testing of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex.

Experimental dermatology

Sørensen CB,Andresen BS,Jensen UB,Jensen TG,Jensen PK,Gregersen N,Bolund L

MA5-14428 was used in immunocytochemistry to study the function of keratin 14 mutant proteins associated with the three major subtypes of epidermolysis bullosa simplex

Fri Aug 01 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CK-8; Cytokeratin-8; K8; keratin 8, type II; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

Gene Aliases: CARD2; CK-8; CK8; CYK8; K2C8; K8; KO; KRT8

UniProt ID: (Human) P05787

Entrez Gene ID: (Human) 3856

Function(s)

viral process tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process

Process(es)

structural molecule activity protein binding protein complex binding scaffold protein binding

Cytokeratin 8 Monoclonal Antibody (TS1)

Cytokeratin 8 Antibody (MA5-14428) in WB

Product Details

MA5-14428

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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