Suggested positive control: anti-Fas treated Jurkat (10 ng/mL), antigen standard for ARHGDIB (transient overexpression lysate).
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
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Protein Aliases: Cleavage specific D4-GDI; D4; Ly-GDI; Rho GDI 2; Rho GDP dissociation inhibitor (GDI) beta; Rho GDP-dissociation inhibitor 2; Rho-GDI beta
Gene Aliases: ARHGDIB; D4; GDIA2; GDID4; Ly-GDI; LYGDI; RAP1GN1; RhoGDI2