Invitrogen

DIXDC1 Monoclonal Antibody (OTI6B6)

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DIXDC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DIXDC1 in COS7 cells. Cells were transfected with a plasmid overexpressing DIXDC1 and probed with a DIXDC1 monoclonal antibody (Product # MA5-26153).

DIXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a DIXDC1 monoclonal antibody (Product # MA5-26153).

DIXDC1 Antibody in Western Blot (WB) — Western blot analysis of DIXDC1 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with DIXDC1 (Product # MA5-26153) monoclonal antibody.

Product Details

MA5-26153

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:150	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Non-human primate
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI6B6
Immunogen	Full length human recombinant protein of DIXDC1 produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724214

Target Information

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.