Invitrogen

DIXDC1 Monoclonal Antibody (OTI6B6)

Product Image Gallery

DIXDC1 Antibody in Immunocytochemistry (ICC) — Immunofluorescent analysis of DIXDC1 in COS7 cells. Cells were transfected with a plasmid overexpressing DIXDC1 and probed with a DIXDC1 monoclonal antibody (Product # MA5-26153).

DIXDC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human breast tissue. To expose target proteins, 10mM citric buffer, pH6.0, 120°C for 3min was used. Following antigen retrieval, tissues were probed with a DIXDC1 monoclonal antibody (Product # MA5-26153).

DIXDC1 Antibody in Western Blot (WB) — Western blot analysis of DIXDC1 in HepG2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12 and MCF7 cells using 35 µg per lane. Samples were separated by SDS-PAGE and probed with DIXDC1 (Product # MA5-26153) monoclonal antibody.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100

Immunofluorescence (IF)

1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Western Blot (WB)

1:200-1:2000

Product Specifications

Species Reactivity

Human, Non-human primate

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI6B6

Immunogen

Full length human recombinant protein of DIXDC1 produced in HEK293T cell

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2724214

Target Information

The protein encoded by this gene is a positive regulator of the Wnt signaling pathway. The encoded protein is found associated with gamma tubulin at the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CCD1; Coiled-coil protein DIX1; Coiled-coil-DIX1; DIX domain containing 1; DIX domain-containing protein 1; DIXDC1; Dixin; KIAA1735

Gene Aliases: CCD1; DIXDC1; EGK_06890; KIAA1735

UniProt ID: (Human) Q155Q3

Entrez Gene ID: (Human) 85458, (Rhesus monkey) 710847

Molecular Function: enzyme modulator signaling molecule