Invitrogen

DLK1 Monoclonal Antibody (3A10)

Product Image Gallery

DLK1 Antibody — Published figure using DLK1 monoclonal antibody (Product # MA5-15915)

DLK1 Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of U251 cells using DLK1 monoclonal antibody (Product # MA5-15915) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

DLK1 Antibody in Western Blot (WB) — Western blot analysis of DLK1 using a DLK1 monoclonal antibody (Product # MA5-15915) against a human DLK1 (AA: 174-349) recombinant protein.

DLK1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of NIH/3T3 cells using DLK1 monoclonal antibody (Product # MA5-15915) (green) and negative control (red).

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1/200 - 1/400

Immunocytochemistry (ICC)

1:200-1:1000

Immunofluorescence (IF)

1/200 - 1/1000

Western Blot (WB)

1/500 - 1/2000

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3A10

Immunogen

Purified recombinant fragment of human DLK1 expressed in E. Coli.

Conjugate

Unconjugated

Form

Liquid

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11155588

Product Specific Information

MA5-15915 targets DLK1 in FACS, IF, and WB applications and shows reactivity with Human samples.

The MA5-15915 immunogen is purified recombinant fragment of human DLK1 expressed in E. Coli. .

MA5-15915 detects DLK1 which has a predicted molecular weight of approximately 41kDa.

Target Information

This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Adipocyte differentiation inhibitor protein; delta-like 1 homolog; DLK, Delta1, FA1, PREF1, Pref-1, ZOG, pG2, DLK-1; DLK-1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; Pref-1; Protein delta homolog 1; secredeltin; stromal cell derived protein 1

Gene Aliases: AW742678; Delta1; DLK; DLK-1; DLK1; DlkI; FA1; Ly107; Peg9; pG2; Pref-1; PREF1; Scp-1; SCP1; ZOG

UniProt ID: (Human) P80370, (Mouse) Q09163

Entrez Gene ID: (Human) 8788, (Mouse) 13386

Molecular Function: extracellular matrix protein extracellular matrix structural protein membrane-bound signaling molecule receptor signaling molecule