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          • Primary Antibodies ›
          • DNA-PK Antibodies

          Invitrogen

          DNA-PK Monoclonal Antibody (18-2), Biotin

          33 References
          View all (48) DNA-PK antibodies
          Datasheet
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          Cite DNA-PK Monoclonal Antibody (18-2), Biotin

          Datasheet
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          Tech Support
          • Antibody Testing Data (3)
          DNA-PK Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          DNA-PK Antibody (MA5-13235) in WB

          Western blot of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13235) on LS174T Cells. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          MA5-13235

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1-2 µg/mL
          View 25 publications 25 publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:100
          -

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Immunoprecipitation (IP)

          2 µg/mL
          View 3 publications 3 publications

          ChIP assay (ChIP)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Gel Shift (GS)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Rat

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          18-2

          Immunogen

          Human DNA-dependent Protein Kinase purified from HeLa cells
          View immunogen

          Conjugate

          Biotin
          • Unconjugated

          Form

          Liquid

          Concentration

          0.2 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 0.2% BSA

          Contains

          0.09% sodium azide

          Storage conditions

          4° C

          RRID

          AB_10982399

          Product Specific Information

          MA5-13235 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

          The MA5-13235 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.

          Target Information

          DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

          View more View less

          Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

          View more View less

          UniProt ID: (Human) P78527

          View more View less

          Entrez Gene ID: (Human) 5591, (Rat) 360748

          View more View less

          Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

          Function(s)
          somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation telomere maintenance double-strand break repair via homologous recombination immunoglobulin production protein phosphorylation response to ionizing radiation phosphorylation lymphocyte differentiation V(D)J recombination
          Process(es)
          double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding molecular_function DNA binding kinase activity
          It has to be done as per old AB suggested Products section.
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