Invitrogen

DNA-PK Monoclonal Antibody (18-2), Biotin

FIGURE: 1 / 3

DNA-PK Antibody (MA5-13235) in WB

Western blot of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13235) on LS174T Cells.

Product Image Gallery

DNA-PK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human tonsil stained with DNAKcs antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lymphocytes.

DNA-PK Antibody in Immunoprecipitation (IP) — Immunoprecipitation of DNA-PKcs using DNA-PKcs Monoclonal Antibody (Product # MA5-13235) on denatured Human LS174T Cells.

Product Details

MA5-13235

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 3 publications 3 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

18-2

Immunogen

Human DNA-dependent Protein Kinase purified from HeLa cells

View immunogen

Conjugate

Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10982399

Product Specific Information

MA5-13235 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13235 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.

Target Information

DNA-PK is a heterotrimeric, DNA-dependent protein kinase that initiates signaling cascades in response to DNA damage. DNA-PK is an atypical kinase, closely related to the PI3 kinases, and is often potently inhibited by small molecules designed to target PI3 kinases. DNA-PK encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for DNA-PK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

p21 promotes error-free replication-coupled DNA double-strand break repair.

Nucleic acids research

Mauro M,Rego MA,Boisvert RA,Esashi F,Cavallo F,Jasin M,Howlett NG

MA5-13235 was used in western blot to study the role of p21 in error-free replication-coupled DNA double-strand break repair.

Sat Sep 01 00:00:00 EDT 2012

Gefitinib radiosensitizes stem-like glioma cells: inhibition of epidermal growth factor receptor-Akt-DNA-PK signaling, accompanied by inhibition of DNA double-strand break repair.

International journal of radiation oncology, biology, physics

Kang KB,Zhu C,Wong YL,Gao Q,Ty A,Wong MC

MA5-13235 was used in western blot to study the molecular mechanism by which gefitinib radiosensitizes brain tumor stem cells

Tue May 01 00:00:00 EDT 2012

Ku counteracts mobilization of PARP1 and MRN in chromatin damaged with DNA double-strand breaks.

Nucleic acids research

Cheng Q,Barboule N,Frit P,Gomez D,Bombarde O,Couderc B,Ren GS,Salles B,Calsou P

MA5-13235 was used in western blot to study the role of PARP1 and MRN proteins in the repair of DNA double strand breaks

Thu Dec 01 00:00:00 EST 2011

The Ku-dependent non-homologous end-joining pathway contributes to low-dose radiation-stimulated cell survival.

Journal of cellular physiology

Yu X,Wang H,Wang P,Chen BP,Wang Y

MA5-13235 was used in western blot to study low-dose radiation induction of cell survival and the mechanistic role of Ku-dependent non-homologous end joining

Tue Feb 01 00:00:00 EST 2011

A DNA-dependent stress response involving DNA-PK occurs in hypoxic cells and contributes to cellular adaptation to hypoxia.

Journal of cell science

Bouquet F,Ousset M,Biard D,Fallone F,Dauvillier S,Frit P,Salles B,Muller C

MA5-13235 was used in immunocytochemistry and western blot to study a DNA-dependent stress response involving DNA-PK in hypoxic cells that contributes to cellular adaptation to hypoxia

Wed Jun 01 00:00:00 EDT 2011

Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes.

Genes & development

Takai H,Xie Y,de Lange T,Pavletich NP

MA5-13235 was used in western blot to study the role of Tel2 in the Hsp90-dependent maturation of mTOR and ATR complexes

Wed Sep 15 00:00:00 EDT 2010

Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair.

Cell cycle (Georgetown, Tex.)

Velkova A,Carvalho MA,Johnson JO,Tavtigian SV,Monteiro AN

MA5-13235 was used in western blot to study the interaction between the BRCA protein and filamin A and its role in efficient DNA repair

Thu Apr 01 00:00:00 EDT 2010

Defective Artemis causes mild telomere dysfunction.

Genome integrity

Yasaei H,Slijepcevic P

MA5-13235 was used in western blot to study the role of defective Artemis in telomere dysfunction

Wed May 26 00:00:00 EDT 2010

The effect of DNA-dependent protein kinase on adeno-associated virus replication.

PloS one

Choi YK,Nash K,Byrne BJ,Muzyczka N,Song S

MA5-13235 was used in blocking or activating experiment to study the role of DNA-dependent protein kinase in adeno-associated virus replication

Mon Dec 20 00:00:00 EST 2010

ARTEMIS nuclease facilitates apoptotic chromatin cleavage.

Cancer research

Britton S,Frit P,Biard D,Salles B,Calsou P

MA5-13235 was used in western blot to study the role of ARTEMIS nuclease in apoptotic chromatin cleavage

Thu Oct 15 00:00:00 EDT 2009

Adeno-associated virus site-specific integration is mediated by proteins of the nonhomologous end-joining pathway.

Journal of virology

Daya S,Cortez N,Berns KI

MA5-13235 was used in immunoprecipitation to study the mechanism of adeno-associated virus site-specific integration

Sun Nov 01 00:00:00 EDT 2009

DNA-dependent protein kinase is a novel interaction partner for Ets-1 isoforms.

Biochemical and biophysical research communications

Choul-li S,Drobecq H,Aumercier M

MA5-13235 was used in western blot to study the interaction of DNA-PK with the Ets-1 transcription factor

Fri Dec 18 00:00:00 EST 2009

Association of autoantibodies with Ku and DNA repair proteins in connective tissue diseases.

Rheumatology (Oxford, England)

Schild-Poulter C,Su A,Shih A,Kelly OP,Fritzler MJ,Goldstein R,Haché RJ

MA5-13235 was used in ChIP assay to study autoantibodies to Ku and DNA repair proteins in connective tissue diseases

Fri Feb 01 00:00:00 EST 2008

Analytical biochemistry of DNA--protein assemblies from crude cell extracts.

Nucleic acids research

Hégarat N,Cardoso GM,Rusconi F,Francois JC,Praseuth D

MA5-13235 was used in western blot to develop an efficient method for the purification of DNA-protein complexes from crude extracts

Wed Aug 15 00:00:00 EDT 2007

The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination.

Proceedings of the National Academy of Sciences of the United States of America

Kiefer K,Oshinsky J,Kim J,Nakajima PB,Bosma GC,Bosma MJ

MA5-13235 was used in western blot to study the mechanism for Ig class-switch recombination

Tue Feb 20 00:00:00 EST 2007

Interplay between Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends in the cell.

The Journal of biological chemistry

Wu PY,Frit P,Malivert L,Revy P,Biard D,Salles B,Calsou P

MA5-13235 was used in western blot to study the roles of Cernunnos-XLF and nonhomologous end-joining proteins at DNA ends

Fri Nov 02 00:00:00 EDT 2007

DNA end joining activity is reduced in Alzheimer's disease.

Neurobiology of aging

Shackelford DA

MA5-13235 was used in western blot to study the role of DNA-dependent protein kinase in reduced DNA end joining activity in Alzheimer's disease

Sat Apr 01 00:00:00 EST 2006

The metallo-beta-lactamase/beta-CASP domain of Artemis constitutes the catalytic core for V(D)J recombination.

The Journal of experimental medicine

Poinsignon C,Moshous D,Callebaut I,de Chasseval R,Villey I,de Villartay JP

MA5-13235 was used in western blot to study the role of the metallo-beta-lactamase/beta-CASP domain of Artemis as the catalytic core for V(D)J recombination

Mon Feb 02 00:00:00 EST 2004

DNA-dependent PK inhibits adeno-associated virus DNA integration.

Proceedings of the National Academy of Sciences of the United States of America

Song S,Lu Y,Choi YK,Han Y,Tang Q,Zhao G,Berns KI,Flotte TR

MA5-13235 was used in western blot to study the inhibitory effect of DNA-PK on adeno-associated virus integration in mouse liver in vitro and in vivo

Tue Feb 17 00:00:00 EST 2004

Reduced DNA-dependent protein kinase activity in two cell lines derived from adult cancer patients with late radionecrosis.

Oncogene

Loong SL,Korzh S,Price A

MA5-13235 was used in western blot to study the reduced DNA-PK activity of two immortalized lymphoblastoid cell lines from adult cancer patients suffering late radionecrosis and the significance for radionecrosis susceptibility

Thu Jul 15 00:00:00 EDT 2004

Mutational analysis of terminal deoxynucleotidyltransferase-mediated N-nucleotide addition in V(D)J recombination.

Journal of immunology (Baltimore, Md. : 1950)

Repasky JA,Corbett E,Boboila C,Schatz DG

MA5-13235 was used in immunoprecipitation to study the role of terminal deoxynucleotidyltransferase in V(D)J recombination

Sat May 01 00:00:00 EDT 2004

Backup pathways of NHEJ are suppressed by DNA-PK.

Journal of cellular biochemistry

Perrault R,Wang H,Wang M,Rosidi B,Iliakis G

MA5-13235 was used in western blot to study the suppression of alternative, backup pathways of non-homolgous end joining by DNA-PK

Thu Jul 01 00:00:00 EDT 2004

Ku70/Ku80 and DNA-dependent protein kinase catalytic subunit modulate RAG-mediated cleavage: implications for the enforcement of the 12/23 rule.

The Journal of biological chemistry

Sawchuk DJ,Mansilla-Soto J,Alarcon C,Singha NC,Langen H,Bianchi ME,Lees-Miller SP,Nussenzweig MC,Cortes P

MA5-13235 was used in western blot to study the role of Ku70/80 and DNA-PK in regulating RAG-mediated cleavage and the significance for the 12/23 rule specificity

Fri Jul 09 00:00:00 EDT 2004

Possible anti-recombinogenic role of Bloom's syndrome helicase in double-strand break processing.

Nucleic acids research

Onclercq-Delic R,Calsou P,Delteil C,Salles B,Papadopoulo D,Amor-Guéret M

MA5-13235 was used in western blot to study the potential anti-recombinogenic role of BLM DNA helicase in double-strand break processing

Sat Nov 01 00:00:00 EST 2003

Biochemical evidence for Ku-independent backup pathways of NHEJ.

Nucleic acids research

Wang H,Perrault AR,Takeda Y,Qin W,Wang H,Iliakis G

MA5-13235 was used in western blot to study alternative backup pathways of non-homologous end joining that are independent of Ku

Mon Sep 15 00:00:00 EDT 2003

Regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle.

American journal of physiology. Cell physiology

Lebrasseur NK,Coté GM,Miller TA,Fielding RA,Sawyer DB

MA5-13235 was used in western blot to study the regulation of neuregulin/ErbB signaling by contractile activity in skeletal muscle

Thu May 01 00:00:00 EDT 2003

Expression of Ku86 confers favorable outcome of tonsillar carcinoma treated with radiotherapy.

Head & neck

Friesland S,Kanter-Lewensohn L,Tell R,Munck-Wikland E,Lewensohn R,Nilsson A

MA5-13235 was used in immunohistochemistry to study the correlation between Ku86 expression and favorable outcome in tonsillar carcinoma treated with radiotherapy

Tue Apr 01 00:00:00 EST 2003

Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation.

The Journal of biological chemistry

Udayakumar D,Bladen CL,Hudson FZ,Dynan WS

MA5-13235 was used in western blot to study two biochemically distinct pathways of non-homologous end joining that are differentially regulated by DNA-PK

Fri Oct 24 00:00:00 EDT 2003

Hairpin opening and overhang processing by an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination.

Cell

Ma Y,Pannicke U,Schwarz K,Lieber MR

MA5-13235 was used in immunoprecipitation and western blot to study the function of an Artemis/DNA-dependent protein kinase complex in nonhomologous end joining and V(D)J recombination

Fri Mar 22 00:00:00 EST 2002

The p53 response to DNA damage in vivo is independent of DNA-dependent protein kinase.

Molecular and cellular biology

Jhappan C,Yusufzai TM,Anderson S,Anver MR,Merlino G

MA5-13235 was used in western blot to study whether DNA-dependent protein kinase is involved in the p53-mediated response to DNA damage

Thu Jun 01 00:00:00 EDT 2000

Interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase.

The Journal of biological chemistry

Chen L,Trujillo K,Sung P,Tomkinson AE

MA5-13235 was used in western blot to study the interactions of the DNA ligase IV-XRCC4 complex with DNA ends and the DNA-dependent protein kinase

Fri Aug 25 00:00:00 EDT 2000

Ku antigen-DNA conformation determines the activation of DNA-dependent protein kinase and DNA sequence-directed repression of mouse mammary tumor virus transcription.

Molecular and cellular biology

Giffin W,Gong W,Schild-Poulter C,Haché RJ

MA5-13235 was used in EMSA to study the mechanism by which Ku antigen-DNA conformation modulates the repression of MMTV transcription

Tue Jun 01 00:00:00 EDT 1999

A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.

Immunity

Gao Y,Chaudhuri J,Zhu C,Davidson L,Weaver DT,Alt FW

MA5-13235 was used in western blot to study the DNA-PK-independent functions of Ku70 and Ku80 in V(D)J recombination

Tue Sep 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

View more View less

Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

View more View less

UniProt ID: (Human) P78527

View more View less

Entrez Gene ID: (Human) 5591, (Rat) 360748

View more View less

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

Function(s)

somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation telomere maintenance double-strand break repair via homologous recombination immunoglobulin production protein phosphorylation response to ionizing radiation phosphorylation lymphocyte differentiation V(D)J recombination

Process(es)

double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding molecular_function DNA binding kinase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

DNA-PK Monoclonal Antibody (18-2), Biotin

DNA-PK Antibody (MA5-13235) in WB

Product Details

MA5-13235

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!