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Invitrogen

DNA-PK Monoclonal Antibody (18-2), Biotin

33 References
View all (30) DNA-PK antibodies
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Product Details

Tested Applications
Dilution
Immunofluorescence (IF)
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
1:50-100
Immunoprecipitation (IP)
2 µg/mL
Western Blot (WB)
1-2 µg/mL
Published Applications
Western Blot (WB)
See 25 publications below
Immunocytochemistry (ICC)
See 1 publication below
Neutralization (Neu)
See 1 publication below
Immunoprecipitation (IP)
See 3 publications below
ChIP assay (ChIP)
See 1 publication below
Immunohistochemistry (IHC)
See 1 publication below
Gel Shift (GS)
See 1 publication below
Product Specifications
Species Reactivity
Human, Rat
Published species
Human, Mouse
Host / Isotype
Mouse / IgG1
Class
Monoclonal
Type
Antibody
Clone
18-2
Immunogen
Human DNA-dependent Protein Kinase purified from HeLa cells
Conjugate
Biotin
Form
Liquid
Concentration
0.2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
RRID
AB_10982399

Product Specific Information

MA5-13235 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13235 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.

Target Information

DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: complementing 1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; hyper-radiosensitivity of murine scid mutation; hyper-radiosensitivity of murine scid mutation, complementing 1; p460

Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7

UniProt ID: (Human) P78527

Entrez Gene ID: (Human) 5591, (Rat) 360748

Molecular Function: kinase non-receptor serine/threonine protein kinase nucleic acid binding nucleotide kinase protein kinase transferase

Function(s)
somitogenesis negative regulation of protein phosphorylation B cell lineage commitment pro-B cell differentiation T cell lineage commitment negative regulation of immunoglobulin production positive regulation of immune system process double-strand break repair double-strand break repair via nonhomologous end joining cellular protein modification process brain development heart development cell proliferation intrinsic apoptotic signaling pathway in response to DNA damage response to gamma radiation response to activity telomere capping peptidyl-serine phosphorylation protein destabilization positive regulation of type I interferon production cellular response to insulin stimulus T cell differentiation in thymus immunoglobulin V(D)J recombination T cell receptor V(D)J recombination ectopic germ cell programmed cell death regulation of circadian rhythm positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter positive regulation of fibroblast proliferation rhythmic process spleen development thymus development positive regulation of developmental growth regulation of smooth muscle cell proliferation signal transduction involved in mitotic G1 DNA damage checkpoint double-strand break repair via alternative nonhomologous end joining negative regulation of cellular senescence negative regulation of response to gamma radiation telomere maintenance double-strand break repair via homologous recombination immunoglobulin production protein phosphorylation response to ionizing radiation phosphorylation lymphocyte differentiation V(D)J recombination
Process(es)
double-stranded DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding transcription factor binding enzyme binding poly(A) RNA binding molecular_function DNA binding kinase activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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