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Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.5-1 µg/mL | View 3 publications 3 publications |
Immunocytochemistry (ICC/IF) |
1 µg/mL | View 5 publications 5 publications |
Immunoprecipitation (IP) |
1 µg/mL | - |
Product Specifications | |
---|---|
Species Reactivity |
Tag |
Published species |
Tag |
Host/Isotype |
Rabbit / IgG |
Expression System |
EXPI293 |
Class |
Recombinant Polyclonal |
Type |
Antibody |
Clone |
20H18L16, 20H1L23, 8H2L5, 8H8L17 |
Immunogen |
Synthetic peptide (DYKDDDDK) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.5 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.2 |
Contains |
0.09% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2610628 |
This antibody consists of four clones viz. 20H18L16 and 20H1L23 from the N terminal and 8H2L5 and 8H8L17 from the C terminal.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a mixture of recombinant antibodies co-expressed from a library of heavy and light chains, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – the exact population of the heavy and light chains in a recombinant rabbit polyclonal antibody can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
DYKDDDDK Tag (FLAG® tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
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