|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-925 detects human, bovine, rat and mouse DBH. This antibody is not effective for immunohistochemistry in human brain.
PA3-925 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons. In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla, and DBH containing cells of the sympathetic nervous system.
The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. This sequence is highly conserved (>85% homology) in human, bovine and mouse.
DBH (Dopamine-Beta-hydroxylase) is an oxidoreductase belonging to the copper Type II, ascorbate dependent monooxygenase family. DBH is the enzyme responsible for conversion of dopamine to noradrenaline. Tissue specificity consists of chromaffin granules of the adrenal medulla and synaptic vesicles of the sympatheic nervous system. It exists as both a soluble and membrane-bound form in chromaffin granules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Selective loss of catecholaminergic wake active neurons in a murine sleep apnea model.
PA3-925 was used in immunohistochemistry to study the status of catecholaminergic wake-active neurons in mice subjected to long-term intermittent hypoxia protocol.
|Zhu Y,Fenik P,Zhan G,Mazza E,Kelz M,Aston-Jones G,Veasey SC||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:10060)||2007|
Near simultaneous release of classical and peptide cotransmitters from chromaffin cells.
PA3-925 was used in immunocytochemistry to investigate the kinetics of peptide secretion from chromaffin cells.
|Whim MD||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:6637)||2006|