PA3-925 detects human, bovine, rat and mouse DBH. This antibody is not effective for immunohistochemistry in human brain.
PA3-925 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~72 kDa protein representing DBH from rat neurons. In immunohistochemistry and immunofluorescence procedures, it specifically stains chromaffin cells of the adrenal medulla, and DBH containing cells of the sympathetic nervous system.
The PA3-925 immunogen is a synthetic peptide corresponding to residues S(43) E P P E S P F P Y H I P L D P E G T L(62) of rat DBH. This sequence is highly conserved (>85% homology) in human, bovine and mouse.
DBH (Dopamine-Beta-hydroxylase) is an oxidoreductase belonging to the copper Type II, ascorbate dependent monooxygenase family. DBH is the enzyme responsible for conversion of dopamine to noradrenaline. Tissue specificity consists of chromaffin granules of the adrenal medulla and synaptic vesicles of the sympatheic nervous system. It exists as both a soluble and membrane-bound form in chromaffin granules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dopamine beta hydroxylase (dopamine beta-monooxygenase); Dopamine beta-hydroxylase; dopamine beta-hydroxylase (dopamine beta-monooxygenase); Dopamine beta-monooxygenase; Dopamine-Beta-hydroxylase; Soluble dopamine beta-hydroxylase
Gene Aliases: BOS_12212; DBH; DBM; DOPBHY