Invitrogen

Dystrophin Polyclonal Antibody

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Dystrophin Antibody in Immunohistochemistry (IHC) — IHC analysis of horse muscle tissues using Product # PA1-37587. Horse muscle sections (8 µm) were blocked with 5% horse serum in 1X PBS and stained with PA1-37587 (1:50) for 1 hour followed by a fluorophore-conjugated anti-rabbit IgG secondary antibody (red). Tissues were also stained for Myosin Heavy chain type IIA (green). Data courtesy of the Innovators Program.

Dystrophin Antibody in Western Blot (WB) — Western blot analysis of Skeletal Muscle Cancer Tissue using anti-Dystrophin Polyclonal Antibody (Product # PA5-32388). The recommened dilution for this antibody in western blot applications is 1:25.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

Assay Dependent

Immunofluorescence (IF)

Assay Dependent

Immunohistochemistry (Frozen) (IHC (F))

1:400

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Western Blot (WB)

1 µg/mL

Published Applications

Immunocytochemistry (ICC)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Immunohistochemistry (Frozen) (IHC (F))

See 2 publications below

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human dystrophin.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2549858

Product Specific Information

This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immuohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100 per cent of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65 per cent of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: apodystrophin-3; apodystrophin-I; BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; dystrophin, muscular dystrophy; X-linked muscular dystrophy

Gene Aliases: BMD; CMD3B; DMD; DNADMD1; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke

UniProt ID: (Human) P11532, (Mouse) P11531, (Rat) P11530

Entrez Gene ID: (Human) 1756, (Mouse) 13405, (Rat) 24907

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein