Invitrogen

Dystrophin Polyclonal Antibody

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Dystrophin Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Dystrophin using anti-Dystrophin Polyclonal Antibody (Product # PA5-32388) in Skeletal Muscle Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Dystrophin Antibody — Fig. 3. Reduced mass and myofiber area in Sgcg 521DeltaT mice. (A) Body mass was not significantly different among genotypes. (B) Gluteus/hamstring (GH) muscle mass normalized to tibia length was reduced in 521DeltaT mice compared with controls. (C) The total mass from muscle groups normalized to tibia length was significantly reduced in 521DeltaT mice compared with WT controls (quadriceps, gluteus/hamstring, triceps, diaphragm, heart, tibialis anterior muscle groups combined). (D) Average myofiber cross-sectional area (CSA) was significantly smaller in 521DeltaT mice than in WT controls. (E) Percentage of myofibers with >1 internal nuclei was increased in 521DeltaT muscle, with 4-month-old 521DeltaT myofibers having the most internal nuclei. (F) Representative images of 521DeltaT and WT muscle. Anti-dystrophin (green) outlined myofibers. Hoechst (blue) demonstrated nuclei. Data are mean+-s.e.m. * P <0.05 (one-way ANOVA with Tukey; n >=5 mice per group). Scale bars: 50 um.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:400

View 2 publications 2 publications

Immunocytochemistry (ICC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

Bovine, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from C-terminus of human dystrophin.

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2549858

Product Specific Information

This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.

Target Information

Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: apodystrophin-3; apodystrophin-I; Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; dystrophin, muscular dystrophy; GS1-19O24.1; OTTHUMP00000215592; X-linked muscular dystrophy

Gene Aliases: BMD; CMD3B; DMD; DNADMD1; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke

UniProt ID: (Human) P11532, (Mouse) P11531, (Rat) P11530

Entrez Gene ID: (Human) 1756, (Dog) 606758, (Mouse) 13405, (Rat) 24907

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein