Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Immunohistochemistry (IHC) |
- | View 5 publications 5 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100 | - |
Immunohistochemistry (PFA fixed) (IHC (PFA)) |
- | View 1 publication 1 publication |
Immunohistochemistry (Frozen) (IHC (F)) |
1:400 | View 2 publications 2 publications |
Immunocytochemistry (ICC/IF) |
- | View 2 publications 2 publications |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Human, Mouse, Rat |
Published species |
Bovine, Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Synthetic peptide derived from C-terminus of human dystrophin. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.24 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.6, with 1% BSA |
Contains |
0.1% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2549858 |
This antibody is predicted to react with bovine, chicken, canine and porcine based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is skeletal muscle tissue.
Dystrophin is the 427kDa protein product of the DMB/BMD gene located on the X chromosome at position Xp21. Western blotting and immunohistochemistry are the two established methods for the detection of abnormalities of dystrophin expression in muscle biopsies. Dystrophin abnormalities are thought to occur in 100% of patients with DMD/BMD, although genetic abnormalities may only be detected in up to 65% of cases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apodystrophin-3; apodystrophin-I; Duchenne muscular dystrophy (DMD); Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; dystrophin, muscular dystrophy; GS1-19O24.1; Muscular dystrophy Duchenne and Becker types; OTTHUMP00000215592; X-linked muscular dystrophy
Gene Aliases: BMD; CMD3B; DMD; DNADMD1; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke
UniProt ID: (Human) P11532, (Dog) O97592, (Mouse) P11531, (Rat) P11530
Entrez Gene ID: (Human) 1756, (Dog) 606758, (Mouse) 13405, (Rat) 24907
Molecular Function:
ubiquitin-protein ligase
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