Invitrogen

EBP50 Monoclonal Antibody (EBP-10)

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1 Published Figure
1 Reference
View all (17) EBP50 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-19291

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-

Immunohistochemistry (IHC)

-
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Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

EBP-10

Immunogen

Bacterially produced, recombinant, full-length human NHERF1.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074133

Product Specific Information

This antibody reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.

For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes. A suggested positive control for this product is RAJI human lymphoma cell lysate.

Western Blot: Reducing conditions.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

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Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

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UniProt ID: (Human) O14745

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Entrez Gene ID: (Human) 9368

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Function(s)
receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding
Process(es)
renal sodium ion transport protein complex assembly adenylate cyclase-activating dopamine receptor signaling pathway sensory perception of sound negative regulation of cell proliferation regulation of cell shape regulation of cell size negative regulation of platelet-derived growth factor receptor signaling pathway negative regulation of phosphatidylinositol 3-kinase signaling Wnt signaling pathway cAMP-mediated signaling gland morphogenesis microvillus assembly actin cytoskeleton organization negative regulation of cell migration cellular phosphate ion homeostasis regulation of sodium:proton antiporter activity negative regulation of sodium:proton antiporter activity bile acid secretion glutathione transport negative regulation of catenin import into nucleus establishment of nucleus localization regulation of excretion establishment of epithelial cell apical/basal polarity regulation of protein kinase activity negative regulation of mitotic cell cycle establishment of Golgi localization negative regulation of protein kinase B signaling auditory receptor cell stereocilium organization phospholipase C-activating dopamine receptor signaling pathway renal absorption negative regulation of ERK1 and ERK2 cascade establishment of protein localization to plasma membrane renal phosphate ion absorption positive regulation of intrinsic apoptotic signaling pathway
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