Invitrogen

EBP50 Monoclonal Antibody (EBP-10)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (16) EBP50 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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EBP50 Antibody in Western Blot (WB) — Western blot was performed using Anti-EBP50 Monoclonal Antibody (Product # MA1-19291) and a 50 kDa band corresponding to EBP50 was observed along with uncharacterized band across cell lines tested. Membrane enriched extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), SK-BR-3 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), Hep G2 (Lane 6), HEK-293 (Lane 7)and PC-12 (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2ug/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

EBP50 Antibody in Immunohistochemistry (IHC) — Figure 2 Photomicrographs of TMA cores . Photomicrographs of TMA cores (20x). A) NDP, B) BPH, C) NAC, D) HGPIN, E) PCa, and F) Mets. Note the predominantly membranous pattern in BPH, which was noted in cores from 11/15 cases. To a lesser degree, this is also noted in the depicted specimen of HGPIN, although the cytoplasmic staining is also intense, which partially obscures the distinction. In the remainder of depicted cores, the staining is largely cytoplasmic. No membranous staining was noted in any of the metastatic cores.

EBP50 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of EBP50 was observed in MCF-7, SK-BR-3 and T-47D in higher levels in comparison to MDA-MB-231 in low levels using Anti-EBP50 Monoclonal Antibody (Product # MA1-19291) in Western Blot. (doi:https://doi.org/10.3892/or.2017.5649).

Product Details

MA1-19291

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

EBP-10

Immunogen

Bacterially produced, recombinant, full-length human NHERF1.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

RRID

AB_1074133

Product Specific Information

For use in Western blot applications, MA1-19291 should be placed in a vertical incubator for 60 minutes. A suggested positive control for this product is RAJI human lymphoma cell lysate.

Western Blot: Reducing conditions.

Target Information

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immunohistochemical analysis of ezrin-radixin-moesin-binding phosphoprotein 50 in prostatic adenocarcinoma.

BMC urology

Bartholow TL,Becich MJ,Chandran UR,Parwani AV

MA1-19291 was used in immunohistochemistry to investigate the expression of ezrin-radixin-moesin-binding phosphoprotein 50 in normal and pathological tissues

Tue Jun 14 00:00:00 EDT 2011

Immunohistochemical analysis of ezrin-radixin-moesin-binding phosphoprotein 50 in prostatic adenocarcinoma.

BMC urology

Bartholow TL,Becich MJ,Chandran UR,Parwani AV

MA1-19291 was used in immunohistochemistry to investigate the expression of ezrin-radixin-moesin-binding phosphoprotein 50 in normal and pathological tissues

Tue Jun 14 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: EBP50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

Gene Aliases: EBP50; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1

UniProt ID: (Human) O14745

Entrez Gene ID: (Human) 9368

Function(s)

Process(es)

receptor binding protein binding beta-catenin binding chloride channel regulator activity phosphatase binding PDZ domain binding beta-2 adrenergic receptor binding protein complex binding protein complex scaffold protein self-association dopamine receptor binding growth factor receptor binding

EBP50 Monoclonal Antibody (EBP-10)

Product Details

MA1-19291

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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