Invitrogen

EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

EGFR Antibody in Immunocytochemistry (ICC/IF)

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EGFR Antibody (700308) in ICC/IF

Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Antibody (30H45L48), Recombinant Rabbit Monoclonal (Product # 700308) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488... View More

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EGFR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level of expression of EGFR was observed in A-431 compared to A549, HeLa, U-87 MG and HCT116 using EGFR Monoclonal Antibody (Product # 700308) in Western Blot. {RE}

Product Details

700308

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

5 µg/mL

View 5 publications 5 publications

ChIP assay (ChIP)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

30H45L48

Immunogen

proprietary

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532314

Product Specific Information

This antibody is predicted to react with canine, mouse and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Published figure using EGFR recombinant monoclonal antibody (Product # 700308) in Western Blot

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Published figure using EGFR recombinant monoclonal antibody (Product # 700308) in Western Blot

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Published figure using EGFR recombinant monoclonal antibody (Product # 700308) in Western Blot

Tue Sep 07 00:00:00 UTC 2021

Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.

Cancers

Betriu N,Andreeva A,Semino CE

Published figure using EGFR recombinant monoclonal antibody (Product # 700308) in Western Blot

Tue Sep 07 00:00:00 UTC 2021

VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.

Brain sciences

Peña JS,Vazquez M

700308 was used in Immunocytochemistry to show an interplay between VEGF and EGF-R pathways in Müller glia.

Fri May 29 00:00:00 UTC 2020

Multiplexed Analysis for Anti-Epidermal Growth Factor Receptor Tumor Cell Growth Inhibition Based on Quantum Dot Probes.

Analytical chemistry

Ren D,Xia Y,Wang B,You Z

700308 was used in immunocytochemistry to describe a multiplex analysis method to examine anti-EGFR functions in heterogeneous cells

Tue Apr 19 00:00:00 UTC 2016

Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization.

Bioorganic & medicinal chemistry

Toyama K,Mizuguchi T,Nomura W,Tamamura H

700308 was used in western blot to characterize novel EGF receptor inhibitors

Mon Aug 15 00:00:00 UTC 2016

EGF as a New Therapeutic Target for Medulloblastoma Metastasis.

Cellular and molecular bioengineering

Rico-Varela J,Singh T,McCutcheon S,Vazquez M

700308 was used in immunocytochemistry to examine the role of epidermal growth factor in medulloblastoma chemotactic migration

Tue Dec 01 00:00:00 UTC 2015

Detachment of cells adhered on the photoreactive phospholipid polymer surface by photoirradiation and their functionality.

Colloids and surfaces. B, Biointerfaces

Byambaa B,Konno T,Ishihara K

700308 was used in immunocytochemistry to study the utility of a photoreactive phospolipid bilayer for modulating cell adhesion and detachment and the utility for cellular engineering

Fri Mar 01 00:00:00 UTC 2013

Detachment of cells adhered on the photoreactive phospholipid polymer surface by photoirradiation and their functionality.

Colloids and surfaces. B, Biointerfaces

Byambaa B,Konno T,Ishihara K

700308 was used in immunocytochemistry to study the utility of a photoreactive phospolipid bilayer for modulating cell adhesion and detachment and the utility for cellular engineering

Fri Mar 01 00:00:00 UTC 2013

Single-nucleotide polymorphisms associated with symptomatic infection and differential human gene expression in healthy seropositive persons each implicate the cytoskeleton, integrin signaling, and oncosuppression in the pathogenesis of human parvovirus B19 infection.

The Journal of infectious diseases

Kerr JR,Kaushik N,Fear D,Baldwin DA,Nuwaysir EF,Adcock IM

700308 was used in ChIP Assay, Immunocytochemistry, Western Blot to further examine the role of the host response to parvovirus B19 in the development of symptoms and consequences of viral persistence.

Fri Jul 15 00:00:00 UTC 2005

Genome-wide analysis of allele-specific gene expression using oligo microarrays.

Methods in molecular biology (Clifton, N.J.)

Lee MP

700308 was used in Chromatin immunoprecipitation to perform genome-wide analysis of allele-specific gene expression finding that variation in gene expression is fairly common in humans.

Fri Nov 04 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; Urogastrone

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Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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Function(s)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EGFR Recombinant Rabbit Monoclonal Antibody (30H45L48)

EGFR Antibody (700308) in ICC/IF

Product Details

700308

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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