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Immunofluorescence analysis of ERK1 + ERK2 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ERK1 + ERK2 Antibody(44654G) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human , Mouse , Rat , Cattle|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antibody was produced using a synthetic peptide derived from amino acids 317-339 within the carboxyl-terminal half of the human ERK1 protein. The sequence is conserved in human and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 8 publications below|
ERK1 and ERK2 are widely expressed and are involved in the regulation of meiosis, mitosis, and postmitotic functions in differentiated cells. Many different stimuli, including growth factors, cytokines, virus infection, ligands for heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors and transforming agents, activate the ERK1 and ERK2 pathways. When growth factors bind to the receptor tyrosine kinase, Ras interacts with Raf, the serine/threonine protein kinase and activates it as well. Once actived, Raf phosphorylates serine residue in 2 further kinases, MEK1/2, which in turn phosphorylates tyrosine/threonine in extracellular-signal regulated kinase (ERK) 1/2. Upon activation, the ERKs either phosphorylate a number of cytoplasmic targets or migrate to the nucleus, where they phosphorylate and activate a number of transcription factors such as c-Fos and Elk-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells.
44-654G was used in western blot to identify binding partners ANKHD1 in leukemia cells.
|Machado-Neto JA,Lazarini M,Favaro P,de Melo Campos P,Scopim-Ribeiro R,Franchi Junior GC,Nowill AE,Lima PR,Costa FF,Benichou S,Olalla Saad ST,Traina F||Biochimica et biophysica acta (1853:583)||2015|
Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells.
44-654G was used in western blot to determine if nanoparticles inhibit IL-2-dependent T cell proliferation.
|Côté-Maurais G,Bernier J||Toxicology in vitro : an international journal published in association with BIBRA (28:1474)||2014|
Increased ERK and JNK activation and decreased ERK/JNK ratio are associated with long-term organ damage in patients with systemic lupus erythematosus.
44-654G was used in western blot to test if extracellular signal-regulated kinase and c-Jun N-terminal kinase are associated with long-term organ damage in SLE patients.
|Bloch O,Amit-Vazina M,Yona E,Molad Y,Rapoport MJ||Rheumatology (Oxford, England) (53:1034)||2014|
Lyn is a redox sensor that mediates leukocyte wound attraction in vivo.
44-654G was used in western blot to identify Lyn as a redox sensor that recruits neutrophils to wounds in zebrafish larvae.
|Yoo SK,Starnes TW,Deng Q,Huttenlocher A||Nature (480:109)||2011|
Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.
||Ratthé C,Pelletier M,Chiasson S,Girard D||Journal of leukocyte biology (81:1287)||2007|
The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.
||Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R||Gynecologic oncology (102:453)||2006|
TNF-alpha promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway.
||Lokuta MA,Huttenlocher A||Journal of leukocyte biology (78:210)||2005|
Matrix metalloproteinases (MMP), EMMPRIN (extracellular matrix metalloproteinase inducer) and mitogen-activated protein kinases (MAPK): co-expression in metastatic serous ovarian carcinoma.
||Davidson B,Givant-Horwitz V,Lazarovici P,Risberg B,Nesland JM,Trope CG,Schaefer E,Reich R||Clinical & experimental metastasis (20:621)||2003|
AU018647, ERK1, Erk1, p41mapk, Erk2, ERK2, Mtap2k, p44mapk, p40, p41, AA407128, p44-MAPK, p44, BOS_16809, Esrk1, P42MAPK, PRKM3, PRKM2, PRKM1, p44erk1, HS44KDAP, p42mapk, 9030612K14Rik, ERK, Prkm3, Prkm1, Ert2, ERT1, ERT2, Mnk1, MNK1, MAPK2, MAPK1, C78273, P44MAPK, Erk-1, HUMKER1A, P44ERK1, p38, p44-ERK1, ERK-1
ERK-2, MAP kinase 1, MAP kinase 2, MAP kinase isoform p42, MAPK 2, extracellular signal-regulated kinase 2, mitogen-activated protein kinase 1, mitogen-activated protein kinase 2, p42-MAPK, protein tyrosine kinase ERK2, MAP kinase isoform p44, MAPK 1, extracellular signal-regulated kinase 1, extracellular signal-related kinase 1, insulin-stimulated MAP2 kinase, microtubule-associated protein 2 kinase, mitogen-activated protein kinase 3, MAP kinase 3, p44 MAP kinase, pp42/MAP kinase, ERT1, extracellular-signal-regulated kinase 2, mitogen activated protein kinase 1, MAPK 3, mitogen-activated 3, ERK, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, p41, p41mapk, MAPK, Mitogen activated protein kinase, ERK1, Mitogen activated protein kinase 1, Mitogen activated protein kinase 2, p42 MAP Kinase, p44 MAP Kinase