Invitrogen

ERK5 Recombinant Rabbit Monoclonal Antibody (SD2084)

Product Image Gallery

ERK5 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of ERK5 in A549 cells using a ERK5 Monoclonal Antibody (Product # MA5-32389) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

ERK5 Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of ERK5 in Hela cells using a ERK5 Monoclonal antibody (Product # MA5-32389) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ERK5 Antibody in Western Blot (WB) — Western blot analysis of ERK5 in Hela cell lysate using a ERK5 Monoclonal antibody (Product # MA5-32389) at a dilution of 1:1,000.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:50-1:100

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Western Blot (WB)

1:1,000-1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

SD2084

Immunogen

C terminal human ERK5

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 1% BSA, 40% Glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: big MAP kinase 1; BMK-1; BMK1 kinase; E3 ubiquitin-protein ligase TRAF2; ERK-5; extracellular signal regulated kinase 5; extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen activated protein kinase 7; mitogen-activated protein kinase 7; TNF receptor-associated factor 2; Tumor necrosis factor type 2 receptor-associated protein 3

Gene Aliases: BMK-1; BMK1; ERK-5; ERK4; ERK5; Erk5-T; PRKM7

Entrez Gene ID: (Human) 5598, (Mouse) 23939, (Rat) 114509

Molecular Function: kinase non-receptor serine/threonine protein kinase protein kinase transferase