Western blot analysis of Elafin/PI3 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human PI3-transfected 293T cells. Samples were loaded onto a 15% SDS-PAGE gel and probed with an Elafin/PI3 polyclonal antibody (Product # PA5-35878) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 117 of Elafin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% ProClin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
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