Invitrogen

FAS Monoclonal Antibody (DX2)

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Product Details

MA1-20163

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

4-20 µg/mL

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DX2

Immunogen

Human Fas/CD95 cDNA.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

0.01M PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_558307

Product Specific Information

Recommended positive controls: Raji.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization and Purification of Neoplastic Cells of Nodular Lymphocyte Predominant Hodgkin Lymphoma from Lymph Nodes by Flow Cytometry and Flow Cytometric Cell Sorting.

The American journal of pathology

Fromm JR,Thomas A,Wood BL

MA120163 was used in flow cytometry to document and describe lymphocyte predominant cells from lymph nodes involved in nodular lymphocyte predominant Hodgkin lymphoma

Wed Feb 01 00:00:00 EST 2017

Flow sorting and exome sequencing reveal the oncogenome of primary Hodgkin and Reed-Sternberg cells.

Blood

Reichel J,Chadburn A,Rubinstein PG,Giulino-Roth L,Tam W,Liu Y,Gaiolla R,Eng K,Brody J,Inghirami G,Carlo-Stella C,Santoro A,Rahal D,Totonchy J,Elemento O,Cesarman E,Roshal M

MA1-20163 was used in flow cytometry to identify alterations in genes involved in antigen presentation, chromosome integrity, transcriptional regulation, and ubiquitination in Hodgkin and Reed-Sternberg cells

Thu Feb 12 00:00:00 EST 2015

Early induction of polyfunctional simian immunodeficiency virus (SIV)-specific T lymphocytes and rapid disappearance of SIV from lymph nodes of sooty mangabeys during primary infection.

Journal of immunology (Baltimore, Md. : 1950)

Meythaler M,Wang Z,Martinot A,Pryputniewicz S,Kasheta M,McClure HM,O'Neil SP,Kaur A

MA1-20163 was used in flow cytometry to study the contribution of the cellular immune response in maintaining nonpathogenic SIV infection in sooty mangabeys.

Sun May 01 00:00:00 EDT 2011

Increased caspase activity primes human Lyme arthritis synovial γδ T cells for proliferation and death.

Human immunology

Thai PT,Collins CC,Fortner KA,Koenig A,Hayes SM,Budd RC

MA1-20163 was used in flow cytometry to study the turnover dynamics of human γΔ T cells.

Thu Dec 01 00:00:00 EST 2011

Activated memory B cells may function as antigen-presenting cells in the joints of children with juvenile idiopathic arthritis.

Arthritis and rheumatism

Morbach H,Wiegering V,Richl P,Schwarz T,Suffa N,Eichhorn EM,Eyrich M,Girschick HJ

MA1-20163 was used in Flow cytometry/Cell sorting to investigate whether synovial B cells in juvenile idiopathic arthritis display features of antigen-presenting cells.

Tue Nov 01 00:00:00 EDT 2011

Inhibitory TCR coreceptor PD-1 is a sensitive indicator of low-level replication of SIV and HIV-1.

Journal of immunology (Baltimore, Md. : 1950)

Salisch NC,Kaufmann DE,Awad AS,Reeves RK,Tighe DP,Li Y,Piatak M,Lifson JD,Evans DT,Pereyra F,Freeman GJ,Johnson RP

MA1-20163 was used in flow cytometry to assess the antigenic threshold for the maintenance of PD-1 expression on virus-specific T cells

Fri Jan 01 00:00:00 EST 2010

CLTA-4 blockade in vivo promotes the generation of short-lived effector CD8 T cells and a more persistent central memory CD4 T cell response.

Journal of medical primatology

Hokey DA,Yan J,Hirao LA,Dai A,Boyer JD,Jure-Kunkel MN,Weiner DB

MA1-20163 was used in flow cytometry to discuss the effects of in vivo CTLA-4 blockade as a part of a DNA vaccination regimen in cynomolgus macaques

Mon Dec 01 00:00:00 EST 2008

Expression of CD8alpha identifies a distinct subset of effector memory CD4+ T lymphocytes.

Immunology

Macchia I,Gauduin MC,Kaur A,Johnson RP

MA1-20163 was used in flow cytometry to determine the frequency, phenotype, and function of peripheral CD4+ CD8+ T cells in rhesus macaques infected with simian immunodeficiency virus

Sun Oct 01 00:00:00 EDT 2006

Role for granulocyte colony-stimulating factor in the generation of human T regulatory type 1 cells.

Blood

Rutella S,Pierelli L,Bonanno G,Sica S,Ameglio F,Capoluongo E,Mariotti A,Scambia G,d'Onofrio G,Leone G

MA1-20163 was used in flow cytometry to measure interleukin-10 and transforming growth factor-beta1 produced by T cells from healthy volunteer donors treated with recombinant human G-CSF.

Tue Oct 01 00:00:00 EDT 2002

Apoptotic signaling through CD95 (Fas/Apo-1) activates an acidic sphingomyelinase.

The Journal of experimental medicine

Cifone MG,De Maria R,Roncaioli P,Rippo MR,Azuma M,Lanier LL,Santoni A,Testi R

MA1-20163 was used in blocking assay to study the intracellular signaling generated after cross-linking of CD95 and apoptosis.

Sat Oct 01 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

UniProt ID: (Human) P25445

Entrez Gene ID: (Human) 355

Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding