Invitrogen

FAS Monoclonal Antibody (DX2)

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

10 µL for 10^6 cells or 1 µg/100 µL blood

Western Blot (WB)

Assay dependent

Published Applications

Flow Cytometry (Flow)

See 5 publications below

Miscellaneous PubMed (MISC)

See 4 publications below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DX2

Immunogen

Human Fas/CD95 cDNA.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

0.01M PBS

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_558307

Product Specific Information

MA1-20163 detects Fas in human samples.

MA1-20163 has been successfully used in FACS and Western blot procedures.

The MA1-20163 immunogen corresponds to human Fas/CD95 cDNA which was transfected into mouse L cells.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. One of the most studied receptors include the Fas receptor. Apoptosis mediated by both signaling cascades results in activation of a family of cystein proteases known as caspases. Engagement of Fas by its ligand, or by an appropriate antibody, results in the rapid induction of programmed cell death in susceptible cell lines. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ALPS1A; APO-1; Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; TNF receptor superfamily member 6; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

UniProt ID: (Human) P25445

Entrez Gene ID: (Human) 355

Molecular Function: cytokine receptor receptor tumor necrosis factor receptor