Display Info:
cookie.CK_ISO_CODE.value:us
cookie.CK_LANG_CODE.value:en
cookie.CK_DISPLAY_TYPE.value:
pageContext.request:org.apache.struts2.dispatcher.StrutsRequestWrapper@5d7646d9
Request attributes: pageContext.request.scheme:http(not used)
syndicationProtocol:https
syndicationExtensionPrefix:
syndicationDisplayType:

Invitrogen

FOXP3 Monoclonal Antibody (NRRF-30), eBioscience™

6 Published Figures
19 References
View all (109) FOXP3 antibodies
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support
635D49A6-4B57-425E-8DC4-6BA1AEB7B473 Created with sketchtool. Datasheet
433BB748-DC81-4FE4-83AD-384F97313745 Created with sketchtool. Create Citation
C488C313-B559-4BFD-B17D-A8B65492815A Created with sketchtool. Tech Support

Product Details

Tested Applications
Dilution
Immunohistochemistry (Frozen) (IHC (F))
10 µg/mL
Western Blot (WB)
10 µg/mL
Published Applications
Flow Cytometry (Flow)
See 10 publications below
Miscellaneous PubMed (MISC)
See 1 publication below
Western Blot (WB)
See 2 publications below
Immunocytochemistry (ICC)
See 1 publication below
Immunofluorescence (IF)
See 2 publications below
Immunohistochemistry (Frozen) (IHC (F))
See 1 publication below
Immunohistochemistry (IHC)
See 2 publications below
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host / Isotype
Rat / IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
NRRF-30
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
RRID
AB_529583

Product Specific Information

Description: The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+/CD25- cells imparts a Treg phenotype in these cells.



Immunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.



Intracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers (cat. 00-5523) and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl/6 strain and approximately 5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+/CD25+ cells and 4% of the CD4+/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3 cat. 71-5775), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Applications Reported: This NRRF-30 antibody has been reported for use in immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested: This NRRF-30 antibody has been used at 2 µg/mL for the western blot analysis of Foxp3 expression in mouse splenocytes total cell lysates. This NRRF-30 antibody has been used in immunohistochemical staining of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: AIID; DIETER; Forkhead box P3; Forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; foxp3; IPEX; JM2; PIDX; SCURFIN; scurfy; XPID

Gene Aliases: Foxp3; JM2; scurfin; sf

UniProt ID: (Mouse) Q99JB6

Entrez Gene ID: (Mouse) 20371

Molecular Function: DNA binding protein nucleic acid binding transcription factor

Function(s)
negative regulation of transcription from RNA polymerase II promoter B cell homeostasis cytokine production myeloid cell homeostasis CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment T cell mediated immunity tolerance induction tolerance induction to self antigen regulation of immunoglobulin production positive regulation of T cell tolerance induction regulation of T cell anergy positive regulation of T cell anergy negative regulation of chronic inflammatory response negative regulation of T cell cytokine production positive regulation of peripheral T cell tolerance induction transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of cell proliferation anatomical structure morphogenesis positive regulation of gene expression negative regulation of histone deacetylation negative regulation of NF-kappaB transcription factor activity negative regulation of interferon-gamma production negative regulation of interleukin-10 production negative regulation of interleukin-17 production negative regulation of interleukin-2 production negative regulation of interleukin-4 production negative regulation of interleukin-5 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of interleukin-4 production negative regulation of CREB transcription factor activity positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation positive regulation of transforming growth factor beta1 production positive regulation of immature T cell proliferation in thymus positive regulation of histone acetylation negative regulation of histone acetylation negative regulation of cytokine biosynthetic process T cell activation negative regulation of T cell proliferation negative regulation of sequence-specific DNA binding transcription factor activity regulatory T cell differentiation negative regulation of interferon-gamma biosynthetic process negative regulation of interleukin-2 biosynthetic process positive regulation of regulatory T cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of isotype switching to IgE isotypes regulation of isotype switching to IgG isotypes negative regulation of lymphocyte proliferation negative regulation of cytokine secretion negative regulation of inflammatory response negative regulation of immune response T cell receptor signaling pathway negative regulation of T-helper 17 cell differentiation
Process(es)
core promoter binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NFAT protein binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

We're here to help

Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

Contact tech support
Display Info:
cookie.CK_ISO_CODE.value:us
cookie.CK_LANG_CODE.value:en
cookie.CK_DISPLAY_TYPE.value:
pageContext.request.scheme:http(not used)
syndicationProtocol:https
syndicationExtensionPrefix:
syndicationDisplayType:

 

page info