Invitrogen

FOXP3 Monoclonal Antibody (NRRF-30), eBioscience™

Product Image Gallery

FOXP3 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry of frozen mouse spleen using 10 µg/mL Anti-Mouse Foxp3 Purified followed by Anti-Rat IgG TRITC. Nuclei are stained with DAPI.

FOXP3 Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F)) — Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Immunohistochemistry and Immunohistochemistry (Frozen)

FOXP3 Antibody in Immunohistochemistry (IHC) — Fig. 6 Regulatory T cell infiltration in treated mdx muscles Infiltrating Foxp3 + regulatory T cells in treated muscles of both low-dose (A) and high-dose (B) irradiated and control groups were analyzed by immunohistochemistry at 4, 8, and 12 weeks post-treatment. Number of mice in each group is the same as in Fig. 1 and Fig.4 . Data is expressed as mean +- standard error (SE). * ( P <0.05) indicates significant difference from untreated mdx control.

FOXP3 Antibody in Flow Cytometry (Flow) — Figure 5 Deficiency in T-T antigen presentation leads to reduced Th17/Treg ratios and resistance to EAE (A) Evolution of neurological symptoms (score) and body weight in MOG-immunized WT and Rhog -/- mice. Neurological scores were according to ) Graphs represent the means +- SEMs (n = 8-9 mice per group; paired 2-tailed Student''s t test [weight] and non-parametric matched-pairs signed rank Wilcoxon 2-tailed test [score]). ** p < 0.01; **** p < 0.0001. (B) Two-color contour plots showing expression of Foxp3, CD25, IL-17A, and CCR6 in WT and Rhog -/- mice sacrificed at day 22 (A). Bar graphs show the percentages of Foxp3 + CD25 + and IL17A + CCR6 + T cells (means +- SEMs; n = 8-9 mice per group; unpaired 2-tailed Student''s t test; * p < 0.05; *** p < 0.001). (C) WT and Rhog -/- mice were immunized with MOG, and the draining popliteal lymph nodes were collected 7 days later. The presence of MOG-reactive T cells was analyzed by flow cytometry on the CD4 + CD44 + activated population by incubation with I-A b OVA 329-337 tetramer. Bar plot shows the means +- SEMs (n = 4-5 mice per group; unpaired 2-tailed Student''s t test. ns, not significant). (D) Experimental setup of the bone-marrow adoptive transfer experiment. (E) BM reconstitution was tested in the blood of chimeric mice before immunization with MOG. Bar plots show the percentage (means +- SEMs) of CD4 T, CD8 T, and B cells within the white blood cell population in bone marrow chimeras and, as reference, in Cd3e -/- and WT

Product Details

14-4771-80

Applications	Tested Dilution	Publications
Western Blot (WB)	10 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Immunohistochemistry (Frozen) (IHC (F))	10 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 2 publications 2 publications
Flow Cytometry (Flow)	-	View 15 publications 15 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse
Host/Isotype	Rat / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	NRRF-30
Conjugate	Unconjugated PE
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_529583

Product Specific Information

Description: The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+/CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.

Intracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers (cat. 00-5523) and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl/6 strain and approximately 5% in the BALB/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+/CD25+ cells and 4% of the CD4+/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse/rat Foxp3 cat. 71-5775), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.

Please see our FAQ regarding the usage of eBioscience Foxp3 reagents.

Applications Reported: This NRRF-30 antibody has been reported for use in immunoblotting (WB), and immunohistology staining of frozen tissue sections.

Applications Tested: This NRRF-30 antibody has been used at 2 µg/mL for the western blot analysis of Foxp3 expression in mouse splenocytes total cell lysates. This NRRF-30 antibody has been used in immunohistochemical staining of frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Antigen presentation between T cells drives Th17 polarization under conditions of limiting antigen.

Cell reports

Boccasavia VL,Bovolenta ER,Villanueva A,Borroto A,Oeste CL,van Santen HM,Prieto C,Alonso-López D,Diaz-Muñoz MD,Batista FD,Alarcón B

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Tue Mar 16 00:00:00 EDT 2021

Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.

Nature

Yao W,Rose JL,Wang W,Seth S,Jiang H,Taguchi A,Liu J,Yan L,Kapoor A,Hou P,Chen Z,Wang Q,Nezi L,Xu Z,Yao J,Hu B,Pettazzoni PF,Ho IL,Feng N,Ramamoorthy V,Jiang S,Deng P,Ma GJ,Den P,Tan Z,Zhang SX,Wang H,Wang YA,Deem AK,Fleming JB,Carugo A,Heffernan TP,Maitra A,Viale A,Ying H,Hanash S,DePinho RA,Draetta GF

14-4771 was used in Immunohistochemistry-immunofluorescence to investigate if a mechanistic link between KRAS* signaling and a targetable molecule driving nutrient salvage pathway in pancreatic ductal adenocarcinoma exists.

Mon Apr 01 00:00:00 EDT 2019

Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity.

International journal of oncology

Yoshida M,Taguchi A,Kawana K,Ogishima J,Adachi K,Kawata A,Nakamura H,Sato M,Fujimoto A,Inoue T,Tomio K,Mori M,Nagamatsu T,Arimoto T,Koga K,Hiraike OW,Oda K,Kiyono T,Osuga Y,Fujii T

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Thymic exosomes promote the final maturation of thymocytes.

Scientific reports

Lundberg V,Berglund M,Skogberg G,Lindgren S,Lundqvist C,Gudmundsdottir J,Thörn K,Telemo E,Ekwall O

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Tue Nov 08 00:00:00 EST 2016

Photothermal therapy with immune-adjuvant nanoparticles together with checkpoint blockade for effective cancer immunotherapy.

Nature communications

Chen Q,Xu L,Liang C,Wang C,Peng R,Liu Z

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Fri Oct 21 00:00:00 EDT 2016

Agonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma.

Journal for immunotherapy of cancer

Patel MA,Kim JE,Theodros D,Tam A,Velarde E,Kochel CM,Francica B,Nirschl TR,Ghasemzadeh A,Mathios D,Harris-Bookman S,Jackson CC,Jackson C,Ye X,Tran PT,Tyler B,Coric V,Selby M,Brem H,Drake CG,Pardoll DM,Lim M

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Wed May 18 00:00:00 EDT 2016

IKKβ acts as a tumor suppressor in cancer-associated fibroblasts during intestinal tumorigenesis.

The Journal of experimental medicine

Pallangyo CK,Ziegler PK,Greten FR

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Mon Dec 14 00:00:00 EST 2015

Foxp3 expression in macrophages associated with RENCA tumors in mice.

PloS one

Devaud C,Yong CS,John LB,Westwood JA,Duong CP,House CM,Denoyer D,Li J,Darcy PK,Kershaw MH

14-4771 was used in Western Blotting to study how tumour-associated macrophages could express Foxp3.

Mon Nov 16 00:00:00 EST 2015

Foxp3 expression in macrophages associated with RENCA tumors in mice.

PloS one

Devaud C,Yong CS,John LB,Westwood JA,Duong CP,House CM,Denoyer D,Li J,Darcy PK,Kershaw MH

14-4771 was used in Western Blotting to study how tumour-associated macrophages could express Foxp3.

Mon Nov 16 00:00:00 EST 2015

Histamine deficiency promotes accumulation of immunosuppressive immature myeloid cells and growth of murine gliomas.

Oncoimmunology

Ahn B,Kohanbash G,Ohkuri T,Kosaka A,Chen X,Ikeura M,Wang TC,Okada H

14-4771 was used in Flow cytometry/Cell sorting to investigate the role of HDC in the glioma microenvironment via maturation of myeloid cells and resulting activation of CD8(+) T cells.

Sun Nov 01 00:00:00 EDT 2015

Autoimmune diabetes is suppressed by treatment with recombinant human tissue Kallikrein-1.

PloS one

Maneva-Radicheva L,Amatya C,Parker C,Ellefson J,Radichev I,Raghavan A,Charles ML,Williams MS,Robbins MS,Savinov AY

14-4771 was used in Immunofluorescence to reveal that the protein, DM199, may benefit type 1 diabetics by attenuating autoimmunity and improving beta cell health.

Wed Jun 10 00:00:00 EDT 2015

Helminth Infection and Commensal Microbiota Drive Early IL-10 Production in the Skin by CD4+ T Cells That Are Functionally Suppressive.

PLoS pathogens

Sanin DE,Prendergast CT,Bourke CD,Mountford AP

14-4771 was used in Flow cytometry/Cell sorting to investigate the role of skin CD4+ T cells in immune regulation after repeated pathogen exposure.

Fri May 01 00:00:00 EDT 2015

STING contributes to antiglioma immunity via triggering type I IFN signals in the tumor microenvironment.

Cancer immunology research

Ohkuri T,Ghosh A,Kosaka A,Zhu J,Ikeura M,David M,Watkins SC,Sarkar SN,Okada H

14-4771 was used in Flow cytometry/Cell sorting to display a potential use of STING agonists for the development of effective immunotherapy.

Mon Dec 01 00:00:00 EST 2014

IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model.

Cancer immunology research

Surana R,Wang S,Xu W,Jablonski SA,Weiner LM

14-4771 was used in Flow cytometry/Cell sorting to demonstrate that IL4 neutralisation enhances the efficacy of trastuzumab by influencing the phenotype of myeloid cells within the tumour microenvironment.

Sat Nov 01 00:00:00 EDT 2014

Combination of an agonistic anti-CD40 monoclonal antibody and the COX-2 inhibitor celecoxib induces anti-glioma effects by promotion of type-1 immunity in myeloid cells and T-cells.

Cancer immunology, immunotherapy : CII

Kosaka A,Ohkuri T,Okada H

14-4771 was used in Flow cytometry/Cell sorting to evaluate the therapeutic effects and underlying mechanisms of a combination regimen with an agonist CD40 mAb and the COX-2 inhibitor celecoxib.

Fri Aug 01 00:00:00 EDT 2014

R-phycoerythrin-conjugated antibodies are inappropriate for intracellular staining of murine plasma cells.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Kim MS,Kim TS

Published figure using FOXP3 monoclonal antibody (Product # 14-4771-80) in Flow Cytometry

Wed May 01 00:00:00 EDT 2013

Rapamycin ameliorates dystrophic phenotype in mdx mouse skeletal muscle.

Molecular medicine (Cambridge, Mass.)

Eghtesad S,Jhunjhunwala S,Little SR,Clemens PR

14-4771 was used in Immunohistochemistry to assess the effects of rapamycin on the pathology of mdx mouse, a model for Duchenne muscular dystrophy.

Sun Mar 25 00:00:00 EDT 2012

Rapamycin ameliorates dystrophic phenotype in mdx mouse skeletal muscle.

Molecular medicine (Cambridge, Mass.)

Eghtesad S,Jhunjhunwala S,Little SR,Clemens PR

14-4771 was used in Immunohistochemistry to assess the effects of rapamycin on the pathology of mdx mouse, a model for Duchenne muscular dystrophy.

Sun Mar 25 00:00:00 EDT 2012

Store-operated Ca2+ entry through ORAI1 is critical for T cell-mediated autoimmunity and allograft rejection.

Journal of immunology (Baltimore, Md. : 1950)

McCarl CA,Khalil S,Ma J,Oh-hora M,Yamashita M,Roether J,Kawasaki T,Jairaman A,Sasaki Y,Prakriya M,Feske S

14-4771 was used in Flow cytometry/Cell sorting to reaffirm the critical role of ORAI1 for T cell function, and provide important insights into the in vivo functions of CRAC channels for T cell-mediated immunity.

Mon Nov 15 00:00:00 EST 2010

Effects of irradiating adult mdx mice before full-length dystrophin cDNA transfer on host anti-dystrophin immunity.

Gene therapy

Eghtesad S,Zheng H,Nakai H,Epperly MW,Clemens PR

14-4771 was used in Immunohistochemistry to demonstrate that dystrophin gene transfer induces anti-dystrophin immunity that are diminished by temporal removal of host immune cells.

Wed Sep 01 00:00:00 EDT 2010

Effects of irradiating adult mdx mice before full-length dystrophin cDNA transfer on host anti-dystrophin immunity.

Gene therapy

Eghtesad S,Zheng H,Nakai H,Epperly MW,Clemens PR

14-4771 was used in Immunohistochemistry to demonstrate that dystrophin gene transfer induces anti-dystrophin immunity that are diminished by temporal removal of host immune cells.

Wed Sep 01 00:00:00 EDT 2010

The initial phase of an immune response functions to activate regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

O'Gorman WE,Dooms H,Thorne SH,Kuswanto WF,Simonds EF,Krutzik PO,Nolan GP,Abbas AK

Wed Jul 01 00:00:00 EDT 2009

The initial phase of an immune response functions to activate regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

O'Gorman WE,Dooms H,Thorne SH,Kuswanto WF,Simonds EF,Krutzik PO,Nolan GP,Abbas AK

Wed Jul 01 00:00:00 EDT 2009

GITR triggering induces expansion of both effector and regulatory CD4+ T cells in vivo.

Journal of immunology (Baltimore, Md. : 1950)

van Olffen RW,Koning N,van Gisbergen KP,Wensveen FM,Hoek RM,Boon L,Hamann J,van Lier RA,Nolte MA

14-4771 was used in Flow cytometry/Cell sorting to study the role of glucocorticoid-induced TNF receptor family-related protein on activated and regulatory T cells.

Mon Jun 15 00:00:00 EDT 2009

Enhanced selection of FoxP3+ T-regulatory cells protects CTLA-4-deficient mice from CNS autoimmune disease.

Proceedings of the National Academy of Sciences of the United States of America

Verhagen J,Gabrysová L,Minaee S,Sabatos CA,Anderson G,Sharpe AH,Wraith DC

14-4771 was used in Immunofluorescence to show that cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) is not required for the peripheral function of FoxP3(+) Treg cells.

Tue Mar 03 00:00:00 EST 2009

Foxp3 processing by proprotein convertases and control of regulatory T cell function.

The Journal of biological chemistry

de Zoeten EF,Lee I,Wang L,Chen C,Ge G,Wells AD,Hancock WW,Ozkaynak E

14-4771 was used in Western Blotting to investigate Foxp3 processing in Tregs.

Fri Feb 27 00:00:00 EST 2009

Bioinformatics

Protein Aliases: Forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

Gene Aliases: Foxp3; JM2; scurfin; sf

UniProt ID: (Mouse) Q99JB6

Entrez Gene ID: (Mouse) 20371

Function(s)