|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Factor IX from human plasma.|
|Storage buffer||0.01M HEPES, pH 7.4, with 0.15M NaCl, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoelectrophoresis (IE)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-74024 detects Factor IX from human samples.
PA1-74024 has been successfully used in ELISA and immunoelectrophoresis applications.
The PA1-74024 immunogen is Factor IX from human plasma.
Factor IX (F.IX, Christmas Factor) is a vitamin K-dependent glycoprotein produced in the liver. Plasma concentration of F.IX is normally around 5 µg/ml (87 nM) in plasma. The biological importance of F.IX is demonstrated in Haemophilia B (Christmas disease), an X-linked congenital bleeding disease resulting from a quantitative (low activity and low antigen) or qualitative (low activity, normal antigen) defect in F.IX function.
In its proenzyme or zymogen form F.IX is a single chain molecule of 55 kDa. It contains two EGF-like domains and an amino-terminal domain containing 12 ?-carboxy-glutamic acid (Gla) residues. These Gla residues allow F.IX to bind divalent metal ions and participate in calcium-dependent binding interactions. The activation of F.IX occurs by limited proteolysis in the presence of calcium by activated factor XI (FXI^a) and/or by a complex of VII^a/tissue factor/phospholipid and activated Factor X between residues Arg(146)-Ala(147) and between Arg(180)-Val(181). The terminal activated product in either case is F.IX^ab, a two-chain enzyme consisting of a heavy chain (28 kDa), a light chain (18 kDa) and an activation peptide product of 11 kDa. F.IX can also be cleaved into inactive products by thrombin and by elastase.
The activity of F.IX^ab in plasma is inhibited by antithrombin and this inhibition is accelerated 1000-fold in the presence of optimal concentrations of heparin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.