Invitrogen

GAA Monoclonal Antibody (2G7)

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GAA Antibody in Immunocytochemistry (ICC) — Immunocytochemistry analysis of GAA in A549 cell. Antigen retrieval was performed with enzyme antigen retrieval (15 min). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 2 µg/mL (overnight, 4°C), followed by Cy3 conjugated goat anti-mouse IgG (30 min, 37°C) and DAPI at a dilution of 1:100.

GAA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GAA in paraffin-embedded human prostatic cancer tissue. Antigen retrieval was performed with EDTA buffer (pH 8.0, epitope retrieval solution). Samples were blocked with 10% goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 1 µg/mL (overnight, 4°C), followed by biotinylated goat anti-mouse IgG (30 min, 37°C) and Strepavidin-Biotin-Complex (SABC) with DAB.

GAA Antibody in Western Blot (WB) — Western blot analysis of GAA in the following samples: Lane 1: human A549 tissue lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human PC-3 whole cell lysates. Samples consisting of 50 µg (reducing conditions) of protein was separated with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V, Resolving gel; 2-3 hrs.), transferred to a Nitrocellulose membrane (150mA, 50-90 min) and washed with TBS-0.1% Tween (3 times, 5 minutes/wash) and blocked with 5% Non-fat Milk/TBS (1.5 hrs., room temperature). The membrane was incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 0.5 µg/mL (overnight, 4°C), followed by goat anti-mouse IgG-HRP and chemiluminescence (ECL) with a dilution of 1:10,000 (1.5 hours, room temperature).

GAA Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of GAA in THP-1 cells. Samples were blocked with 10% normal goat serum and incubated in GAA monoclonal antibody (Product # MA5-36228) at a dilution of 1 µg/1x10^6 cells (30 min, 20°C), followed by Dylight 488 conjugated goat anti-mouse IgG (30 min, 37°C) with a dilution of 5-10 µg/1x10^6 cells (30 min, 20°C). Isotype control antibody (Green line) was mouse IgG (1 µg/1x106) and unlabeled sample (Red line).

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2G7

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin.

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 0.9mg NaCl, 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Sequence of this protein is as follows: MLLLADMDVV NQLVAGGQFR VVKEPL

Target Information

This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: 70 kDa lysosomal alpha-glucosidase; 76 kDa lysosomal alpha-glucosidase; Acid maltase; Aglucosidase alfa; Lysosomal alpha-glucosidase

Gene Aliases: GAA; LYAG

UniProt ID: (Human) P10253

Entrez Gene ID: (Human) 2548

Molecular Function: glucosidase hydrolase