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Product Details
MA5-29285
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
223
Immunogen
Recombinant Human Glucokinase Protein (Leu2-Gln465)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
RRID
AB_2785178
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human Glucokinase.
Target Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP:D-hexose 6-phosphotransferase; Glucokinase; glucokinase (hexokinase 4); hexokinase D, pancreatic isozyme; Hexokinase type IV; Hexokinase-4; Hexokinase-D; HK IV; HK4; MEKKK 2
Gene Aliases: FGQTL3; GCK; GK; GLK; HHF3; HK4; HKIV; HXKP; LGLK; MODY2
UniProt ID: (Human) P35557
Entrez Gene ID: (Human) 2645
Molecular Function:
kinase
cellular glucose homeostasis
glycogen biosynthetic process
fructose 2,6-bisphosphate metabolic process
glycolytic process
regulation of glycolytic process
NADP metabolic process
positive regulation of cytosolic calcium ion concentration
regulation of glucose transport
glucose transport
second-messenger-mediated signaling
positive regulation of insulin secretion
negative regulation of epinephrine secretion
cellular response to insulin stimulus
cellular response to glucose starvation
glucose homeostasis
regulation of potassium ion transport
cellular response to leptin stimulus
negative regulation of gluconeogenesis
positive regulation of glycogen biosynthetic process
positive regulation of glycolytic process
carbohydrate phosphorylation
regulation of insulin secretion
glucose 6-phosphate metabolic process
detection of glucose
lipid homeostasis
canonical glycolysis
calcium ion import
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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