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      GFAP Polyclonal Antibody

      See 27 other GFAP antibodies ›
      GFAP Polyclonal Antibody for Western Blot, IF, ICC and IHC

      Catalog#: PA1-10019 Invitrogen

      Catalog # Size Price (USD) Qty Datasheet
      PA1-10019 100 µL
      309.00
      Please enter a quantity in the range of 1 to 199.
      Suggested Secondary Antibodies

      Anti-Rabbit Secondary Antibodies (264)
      Alexa Fluor® Anti-Rabbit Secondary Antibodies (69)
      HRP Anti-Rabbit Secondary Antibodies (30)

      Product Details

      References

      Bioinformatics

      Documents
      Details
      Tested species reactivity Bovine, Chicken, Horse, Human, Mouse, Pig, Rat
      Published species reactivity Rat, Not Applicable
      Host / Isotype Rabbit / IgG
      Class Polyclonal
      Type Antibody
      Immunogen Full-length recombinant human GFAP protein
      Conjugate Unconjugated
      Form Liquid
      Storage buffer whole serum
      Contains no preservative
      Storage Conditions Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
      Tested Applications Dilution *
      Immunocytochemistry (ICC) 1:1000-1:5000
      Immunofluorescence (IF) 1:1000-1:5000
      Immunohistochemistry (IHC) 1:1000-1:5000
      Western Blot (WB) 1:10,000-1:100,000

      * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.

      Published Applications
      Immunohistochemistry (IHC) See 2 publications below

      Product Specific Information

      By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50kDa corresponding to rodent GFAP and a lower band at ~45kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.

      Background/Target Information

      Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      References

      Immunohistochemistry

      Species Dilution Title Authors Journal (Vol:Pg) Year
      Not Applicable 1:1000 Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease.
      PA1-10019 was used in immunohistochemistry to characterize tau pathology mouse models via obesity, diabetes, and leptin resistance       		Platt TL,Beckett TL,Kohler K,Niedowicz DM,Murphy MP Neuroscience (315:162) 2016
      Rat 1:250 Analgesic effect of minocycline in rat model of inflammation-induced visceral pain.
      PA1-10019 was used in immunohistochemistry to use a rat model of inflammatory visceral pain to study the analgesis effects of the semi-synthetic tetracycline antibiotic minocycline       		Kannampalli P,Pochiraju S,Bruckert M,Shaker R,Banerjee B,Sengupta JN European journal of pharmacology (727:87) 2014

      Documents

      Material safety data sheets (MSDS)

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