Invitrogen

GFAP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

4 References

View all (68) GFAP antibodies

Datasheet

Datasheet

Product Image Gallery

GFAP Antibody — Figure 3. Attenuation of neuronal death without affecting astrogliosis in SNc in OHE rats by NT administration. (a) Representative cresyl-violet-stained images of SNc in SHAM, BDL+VEH, and BDL+NT groups. SNc is depicted with the area bounded with a dotted line. For a clearer visualization, the rectangular boxed areas in upper panels (100x) are magnified as shown in lower panels (400x). Scale bar = 200 and 50 mum in upper and lower panels, respectively. (b) The average number of SNc neurons. Neuronal cell bodies with visible nucleoli were only counted. Representative TH immunohistochemistry images of SNc (c) and striatum (e) and the averaged TH immunoreactivities in SNc (d) and in striatum (f). Scale bar = 20 and 500 mum in panels (c) and (e), respectively. (g) Representative GFAP immunohistochemistry images and (h) the averaged GFAP immunoreactivities in SNc. Scale bar = 50 mum. To quantify the immunoreactivities of TH and GFAP, the randomly selected brain sections contained with SNc or striatum were photographed under the different magnification values. The ROI per high-power field were analyzed by Image J (v.1.49) and averaged per group. For all graphs, data are expressed as percentage of SHAM group ( n = 8 per group). * p < .01, ** p < .01, *** p < .001 versus SHAM group; # p < .05, ## p < .01 versus BDL+VEH group. BDL = bile duct ligation; VEH = vehicle; NT = nortriptyline; SNr = substantia nigra reticulate; NS = statistically not significant; GFAP = glial fibr

GFAP Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of GFAP in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45µM. Free-floating sections were stained with a GFAP polyclonal antibody (Product # PA1-10019) at a dilution of 1:5,000 as seen in green, with an MeCP2 monoclonal antibody at a dilution 1:500, as seen in red, and with a DAPI staining of nuclear DNA as seen in blue. In this image the GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.

GFAP Antibody in Immunohistochemistry (IHC) — Immunohistological analysis of GFAP in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NF-H polyclonal antibody (Product # PA1-10002) at a dilution of 1:5,000 as seen in red, and costained with a GFAP polyclonal antibody (Product # PA1-10019) at a dilution of 1:5,000 as seen in green, and with DAPI staining the nuclear DNA in blue. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

GFAP Antibody in Western Blot (WB) — Western blot was performed using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-10019) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse liver (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GFAP Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of GFAP was observed in Mouse brain, Rat brain in comparison to Mouse liver and Rat liver using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-10019) in Western Blot. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:1000-1:5000

Immunofluorescence (IF)

1:1000-1:5000

Immunohistochemistry (IHC)

1:1000-1:5000

Western Blot (WB)

1:5000

Published Applications

Immunohistochemistry (IHC)

See 3 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Horse, Human, Mouse, Pig, Rat

Published species

Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full-length recombinant human GFAP protein

Conjugate

Unconjugated

Form

Liquid

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1074611

Product Specific Information

By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50 kDa corresponding to rodent GFAP and a lower band at ~45 kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily, and specifically, expressed in astrocytes and certain astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FLJ45472; GFAP; Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

Gene Aliases: AI836096; ALXDRD; BOS_19250; GFAP

UniProt ID: (Bovine) Q28115, (Human) P14136, (Rat) P47819, (Mouse) P03995

Entrez Gene ID: (Bovine) 281189, (Horse) 100033970, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580

Molecular Function: cytoskeletal protein intermediate filament structural protein