|Tested species reactivity||Bovine, Chicken, Horse, Human, Mouse, Pig, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full-length recombinant human GFAP protein|
|Storage buffer||whole serum|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:10,000-1:100,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 2 publications below|
By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50kDa corresponding to rodent GFAP and a lower band at ~45kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease.
PA1-10019 was used in immunohistochemistry to characterize tau pathology mouse models via obesity, diabetes, and leptin resistance
|Platt TL,Beckett TL,Kohler K,Niedowicz DM,Murphy MP||Neuroscience (315:162)||2016|
Analgesic effect of minocycline in rat model of inflammation-induced visceral pain.
PA1-10019 was used in immunohistochemistry to use a rat model of inflammatory visceral pain to study the analgesis effects of the semi-synthetic tetracycline antibiotic minocycline
|Kannampalli P,Pochiraju S,Bruckert M,Shaker R,Banerjee B,Sengupta JN||European journal of pharmacology (727:87)||2014|