Invitrogen

GFAP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

12 Published Figures

18 References

View all (115) GFAP antibodies

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GFAP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GFAP in rat cerebellum section. The rat cerebellum section was obtained following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45µM. Free-floating sections were stained with a GFAP polyclonal antibody (Product # PA1-10019) at a dilution of 1:5,000 as seen in green, with an MeCP2 monoclonal antibody at a dilution 1:500, as seen in red, and with a DAPI staining of nuclear DNA as seen in blue. In this image the GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.

GFAP Antibody in Immunohistochemistry (IHC) — Immunohistological analysis of GFAP in rat cerebellum. The rat cerebellum section was obtained following transcardial perfusion of the rat with 4% paraformaldehyde, brain was post fixed for 24 hours, and cut to 45µM. Free-floating sections were stained with an NF-H polyclonal antibody (Product # PA1-10002) at a dilution of 1:5,000 as seen in red, and costained with a GFAP polyclonal antibody (Product # PA1-10019) at a dilution of 1:5,000 as seen in green, and with DAPI staining the nuclear DNA in blue. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes and other glial cells.

GFAP Antibody in Western Blot (WB) — Western blot was performed using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-10019) and a 50 kDa band corresponding to GFAP was observed across tissues tested except Mouse and Rat liver. Whole cell extracts (30 µg lysate) of Mouse brain (Lane 1), Rat brain (Lane 2), Mouse liver (Lane 3) and Rat liver (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GFAP Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissue tested owing to their inherent genetic constitution. Relative expression of GFAP was observed in Mouse brain, Rat brain in comparison to Mouse liver and Rat liver using Anti-GFAP Rabbit Polyclonal Antibody (Product # PA1-10019) in Western Blot. {RE}

GFAP Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 8. CRISPRa-mediated induction of Fosb is neuron-selective in vivo . A , B , IHC performed for ( A ) NeuN or ( B ) GFAP alongside Fosb demonstrates neuronal selectivity of CRISPRa-mediated Fosb induction. Scale bar = 50 mum. C , Pixel density quantification and cross-correlation analysis reveals a signal overlap between Fosb and NeuN and depletion of signal between Fosb and GFAP ( n = 2 animals with eight regions of interest). All data are expressed as mean +- SEM.

GFAP Antibody in Flow Cytometry (Flow) — Figure 3 Immunophenotype characterization of the representative hWJ-NSCs sample. ( a ) Expression of Nestin and SOX1. P1 gated on the plot represents the cell population of neural stem and progenitor cells (Nestin+/SOX1+) comprising 96.1% of the total cells; ( b ) expression of Ki67 and SOX1. P2 gated on the plot represents the Ki67+/SOX1+ cell population comprising 93.3% of the total cells; ( c ) expression of DCX and nestin. P3 gated on the plot represents glia and neural stem and progenitor cells (Nestin+/DCX-) comprising 93.7% of the total cells; ( d ) expression of Ki67 and SOX2. P4 gated on the plot represents Ki67+/SOX1+ cells comprising 93.3% of the total cells; ( e ) expression of DCX and nestin. P3 gated on the plot represents glia and neural stem and progenitor cells (Nestin+/DCX-) comprising 94.8% of the total cells; ( f ) expression of CD44 and SOX1. P5 gated on the plot represents glial cells (CD44+/SOX1+) comprising 96% of the total cells; ( g ) expression of DCX and GFAP. P6 gated on the plot represents astrocytes (GFAP+/DCX-) comprising 32.9% of the total cells. P7 gated on the plot represents DCX+/GFAP- cell population comprising 1.67% of the total cells; ( h ) expression of CD44 and SOX1. P5 gated on the plot represents glial cells (CD44+/SOX1+) comprising 99% of the total cells; U--unstained cell population gated on all plots. SOX1--sex-determining region Y (SRY) box 1; SOX2--sex-determining region Y (SRY) box 2; GFAP--glial fibrillary acidic protein; DCX-

Product Details

PA1-10019

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000	-
Immunohistochemistry (IHC)	1:1,000-1:5,000	View 11 publications 11 publications
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:1,000-1:5,000	View 5 publications 5 publications
Flow Cytometry (Flow)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Horse, Human, Mouse, Pig, Rat
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Full-length recombinant human GFAP protein View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	whole serum
Contains	5mM sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_1074611

Product Specific Information

By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50 kDa corresponding to rodent GFAP and a lower band at ~45 kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low-dose metformin treatment in the subacute phase improves the locomotor function of a mouse model of spinal cord injury.

Neural regeneration research

Song WY,Ding H,Dunn T,Gao JL,Labastida JA,Schlagal C,Ning GZ,Feng SQ,Wu P

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Low-dose metformin treatment in the subacute phase improves the locomotor function of a mouse model of spinal cord injury.

Neural regeneration research

Song WY,Ding H,Dunn T,Gao JL,Labastida JA,Schlagal C,Ning GZ,Feng SQ,Wu P

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunocytochemistry

Mon Nov 01 00:00:00 EDT 2021

Nicotine induces morphological and functional changes in astrocytes via nicotinic receptor activity.

Glia

Aryal SP,Fu X,Sandin JN,Neupane KR,Lakes JE,Grady ME,Richards CI

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunocytochemistry

Sun Aug 01 00:00:00 EDT 2021

Erythrocyte adenosine A2B receptor prevents cognitive and auditory dysfunction by promoting hypoxic and metabolic reprogramming.

PLoS biology

Qiang Q,Manalo JM,Sun H,Zhang Y,Song A,Wen AQ,Wen YE,Chen C,Liu H,Cui Y,Nemkov T,Reisz JA,Edwards Iii G,Perreira FA,Kellems RE,Soto C,D'Alessandro A,Xia Y

PA1-10019 was used in Immunohistochemistry to determine that erythrocyte ADORA2B-BPGM axis is a key component for anti-aging and anti-age-related functional decline.

Tue Jun 01 00:00:00 EDT 2021

Cellular and molecular outcomes of glutamine supplementation in the brain of succinic semialdehyde dehydrogenase-deficient mice.

JIMD reports

Brown MN,Gibson KM,Schmidt MA,Walters DC,Arning E,Bottiglieri T,Roullet JB

PA1-10019 was used in Immunohistochemistry to determine the impact of glutamine supplementation in the brain of succinic semialdehyde dehydrogenase-deficient mice.

Sun Nov 01 00:00:00 EDT 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

PA1-10019 was used in Immunohistochemistry-immunofluorescence to investigate the role of Rosmarinic acid (RA) in the prevention of traumatic brain injury and the immunohistochemical analysis of IBA-1 and GFAP expressions.

Mon Jun 29 00:00:00 EDT 2020

The effect of rosmarinic acid on deformities occurring in brain tissue by craniectomy method. Histopathological evaluation of IBA-1 and GFAP expressions.

Acta cirurgica brasileira

Özevren H,Deveci E,Tuncer MC

Mon Jun 29 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

PA1-10019 was used in Immunocytochemistry-immunoflourescence to develop a feasible, efficient, and reproducible method for the neural induction of mesenchymal stem cells isolated from Wharton's jelly (hWJ-MSCs).

Tue Mar 17 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Tue Mar 17 00:00:00 EDT 2020

Differentiation of Human Mesenchymal Stem Cells from Wharton's Jelly Towards Neural Stem Cells Using A Feasible and Repeatable Protocol.

Cells

Kruminis-Kaszkiel E,Osowski A,Bejer-Oleńska E,Dziekoński M,Wojtkiewicz J

Tue Mar 17 00:00:00 EDT 2020

Nonivasive quantification of axon radii using diffusion MRI.

eLife

Veraart J,Nunes D,Rudrapatna U,Fieremans E,Jones DK,Novikov DS,Shemesh N

PA1-10019 was used in Immunohistochemistry to enable noninvasive mapping of critical information on axon radii, as well as resolve the long-standing debate on whether axon radii can be quantified.

Wed Feb 12 00:00:00 EST 2020

Neuroprotective Effect of Nortriptyline in Overt Hepatic Encephalopathy Through Attenuation of Mitochondrial Dysfunction.

ASN neuro

Jeong JH,Kim DK,Lee NS,Jeong YG,Kim HW,Kim JS,Han SY

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunohistochemistry

Thu Jul 11 00:00:00 EDT 2019

A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation.

eNeuro

Savell KE,Bach SV,Zipperly ME,Revanna JS,Goska NA,Tuscher JJ,Duke CG,Sultan FA,Burke JN,Williams D,Ianov L,Day JJ

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunofluorescence

Thu Apr 18 00:00:00 EDT 2019

A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation.

eNeuro

Savell KE,Bach SV,Zipperly ME,Revanna JS,Goska NA,Tuscher JJ,Duke CG,Sultan FA,Burke JN,Williams D,Ianov L,Day JJ

Published figure using GFAP polyclonal antibody (Product # PA1-10019) in Immunofluorescence

Thu Apr 18 00:00:00 EDT 2019

Spatial and Sex-Dependent Responses of Adult Endogenous Neural Stem Cells to Alcohol Consumption.

Stem cell reports

McGrath EL,Gao J,Kuo YF,Dunn TJ,Ray MJ,Dineley KT,Cunningham KA,Kaphalia BS,Wu P

PA1-10019 was used in Immunohistochemistry-immunofluorescence to discover the effects of chronic alcohol consumption on the survival of neural stem cells in three difference brain regions.

Tue Dec 12 00:00:00 EST 2017

PloS one

Huang H,Liu Y,Wang L,Li W

PA110019 was used in immunohistochemistry - frozen section to investigate the role of Cxcr5 receptor in age-related macular degeneration in mice.

Thu Aug 17 00:00:00 EDT 2017

Identification of mouse cochlear progenitors that develop hair and supporting cells in the organ of Corti.

Nature communications

Xu J,Ueno H,Xu CY,Chen B,Weissman IL,Xu PX

PA1-10019 was used in Immunohistochemistry to discover progenitor cells in the cochlear of mice which contribute to the hair and supporting cells during development, maturation and repair.

Thu May 11 00:00:00 EDT 2017

Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease.

Neuroscience

Platt TL,Beckett TL,Kohler K,Niedowicz DM,Murphy MP

PA1-10019 was used in immunohistochemistry to characterize tau pathology mouse models via obesity, diabetes, and leptin resistance

Fri Feb 19 00:00:00 EST 2016

Obesity, diabetes, and leptin resistance promote tau pathology in a mouse model of disease.

Neuroscience

Platt TL,Beckett TL,Kohler K,Niedowicz DM,Murphy MP

PA1-10019 was used in immunohistochemistry to characterize tau pathology mouse models via obesity, diabetes, and leptin resistance

Fri Feb 19 00:00:00 EST 2016

Analgesic effect of minocycline in rat model of inflammation-induced visceral pain.

European journal of pharmacology

Kannampalli P,Pochiraju S,Bruckert M,Shaker R,Banerjee B,Sengupta JN

PA1-10019 was used in immunohistochemistry to use a rat model of inflammatory visceral pain to study the analgesis effects of the semi-synthetic tetracycline antibiotic minocycline

Sat Mar 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

Gene Aliases: AI836096; ALXDRD; GFAP

UniProt ID: (Bovine) Q28115, (Human) Q9UFD0, (Rat) P47819, (Mouse) P03995

Entrez Gene ID: (Bovine) 281189, (Horse) 100033970, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580

Function(s)