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Invitrogen
GFAP Polyclonal Antibody
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FIGURE: 1 / 23
GFAP Antibody (PA1-10019) in ICC/IF
Immunofluorescent analysis of GFAP in cortical neuron-glial cell culture. Culture is from an E20 rat that was stained with a GFAP polyclonal antibody (Product # PA1-10019) at a dilution of 1:5,000 as seen in red, with a Vimentin polyclonal antibody at a dilution of 1:10,000 as seen in green, and with DAPI staining of nuclear DNA as seen in blue. In this image the astrocytes containing GFAP only are red, GFAP and Vimentin are golden-yellow, and the fibroblastic cells only containing vimentin are green.
Product Details
PA1-10019
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Horse,
Human,
Mouse,
Pig,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
5mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
By Western blot, two bands are detected in rat cerebellum homogenate: a prominent band at 50 kDa corresponding to rodent GFAP and a lower band at ~45 kDa that most likely represents a proteolytic fragment derived from the GFAP molecule.
Target Information
GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein
Gene Aliases: AI836096; ALXDRD; GFAP
UniProt ID: (Bovine) Q28115, (Human) P14136, (Rat) P47819, (Mouse) P03995
Entrez Gene ID: (Bovine) 281189, (Horse) 100033970, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580
intermediate filament organization
response to wounding
positive regulation of Schwann cell proliferation
negative regulation of neuron projection development
astrocyte development
extracellular matrix organization
neuron projection regeneration
intermediate filament-based process
regulation of neurotransmitter uptake
Bergmann glial cell differentiation
positive regulation of glial cell proliferation
long-term synaptic potentiation
regulation of protein complex assembly
regulation of chaperone-mediated autophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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