Invitrogen

Glypican 1 Polyclonal Antibody

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Glypican 1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Glypican 1 was performed in MCF 7 cells fixed in ice cold MeOH for 5 min. Green: Glypican 1 Polyclonal Antibody (Product # PA5 28055) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Glypican 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Glypican 1 Polyclonal Antibody detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Glypican 1 stained by Glypican 1 Polyclonal Antibody (Product # PA5-28055) diluted at 1:1,000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Glypican 1 Antibody in Western Blot (WB) — Western Blot analysis of Glypican 1 was performed by separating 30 µg of U87-MG whole cell extracts and conditioned medium by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Glypican 1 Polyclonal Antibody (Product # PA5-28055) at a dilution of 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Glypican 1 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of TLR3 was performed in A431 cells using TLR3 Monoclonal Antibody (40C1285) (Product # MA1-25649) (red) at a dilution of 1:100. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Glypican 1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Glypican 1 was performed in MCF-7 whole cell extracts using 5 µg of Glypican 1 Polyclonal Antibody (Product # PA5-28055). Samples were transferred to a membrane and probed with Glypican 1 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.

Glypican 1 Antibody in ELISA (ELISA) — An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 Polyclonal Antibody (Product # PA5-28055) at concentration ranged from 7.5 to 240 ng/mL.

Glypican 1 Antibody in Immunohistochemistry (IHC) — Fig. 4 Sweat particles. Visual characterization of sweat particles. Panels A : TEM, thin sections of plastic embedded pelleted sweat from 4 donors number corresponding to sequencing library (Table 1 ). B : TEM, negative staining of ExoEasy isolated sweat EVs, C : NTA analysis of Exoeasy EVs, D : TEM, Immunostaining of isolated sweat EVs, E: western blot, protein from Exoeasy sweat preparations were stained with anti-CD63 antibody (ab193349). Full size western blot with region selected marked is shown as supplementary Fig. 6

Product Details

PA5-28055

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Flow Cytometry (Flow)	1:50-1:200	-
ELISA (ELISA)	1:1,000-1:10,000	-
Immunoprecipitation (IP)	1:100-1:500	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.58 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545531

Product Specific Information

Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).

Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Characterization of nucleic acids from extracellular vesicle-enriched human sweat.

BMC genomics

Bart G,Fischer D,Samoylenko A,Zhyvolozhnyi A,Stehantsev P,Miinalainen I,Kaakinen M,Nurmi T,Singh P,Kosamo S,Rannaste L,Viitala S,Hiltunen J,Vainio SJ

Published figure using Glypican 1 polyclonal antibody (Product # PA5-28055) in Immunohistochemistry

Wed Jun 09 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12; Secreted glypican-1

Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12

UniProt ID: (Human) P35052, (Mouse) Q9QZF2, (Rat) P35053

Entrez Gene ID: (Human) 2817, (Mouse) 14733, (Rat) 58920

Function(s)

Schwann cell differentiation heparan sulfate proteoglycan catabolic process myelin assembly negative regulation of fibroblast growth factor receptor signaling pathway retinoid metabolic process glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process axon guidance glycosaminoglycan metabolic process positive regulation of skeletal muscle cell differentiation biological_process

Process(es)

copper ion binding fibroblast growth factor binding laminin binding heparan sulfate proteoglycan binding collagen V binding protein binding

Glypican 1 Polyclonal Antibody

Product Details

PA5-28055

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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