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Search Thermo Fisher Scientific
Invitrogen
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
Flow Cytometry (Flow) |
1:50-1:200 | - |
ELISA (ELISA) |
1:1,000-1:10,000 | - |
Immunoprecipitation (IP) |
1:100-1:500 | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Not Applicable |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.58 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2545531 |
Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).
Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12; Secreted glypican-1
Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12
UniProt ID: (Human) P35052, (Mouse) Q9QZF2, (Rat) P35053
Entrez Gene ID: (Human) 2817, (Mouse) 14733, (Rat) 58920
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