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Invitrogen
Glypican 1 Polyclonal Antibody
FIGURE: 1 / 15
Glypican 1 Antibody (PA5-28055) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Glypican 1 was performed in MCF 7 cells fixed in ice cold MeOH for 5 min. Green: Glypican 1 Polyclonal Antibody (Product # PA5 28055) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-28055
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.71 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2545531
Product Specific Information
Recommended positive controls: A431, K562, MCF-7, AsPC-1, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin for 16hr), U87-MG, U87-MG membrane extracts, U87-MG conditioned medium, Mouse brain, mouse fetal brain, A431 (500 units PNGase F for 4 hr), A431 (24 µg/mL Tunicamycin treatment for 16hr), A431 (4 mM Benzyl glucosinolate treatment for 32hr), A431(24 µg/mL Tunicamycin treatment for 16hr and 4 mM Benzyl glucosinolate treatment for 32hr).
Predicted reactivity: Mouse (89%), Rat (88%), Rhesus Monkey (97%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12
Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12
UniProt ID: (Human) P35052, (Mouse) Q9QZF2, (Rat) P35053
Entrez Gene ID: (Human) 2817, (Mouse) 14733, (Rat) 58920
Schwann cell differentiation
heparan sulfate proteoglycan catabolic process
myelin assembly
negative regulation of fibroblast growth factor receptor signaling pathway
retinoid metabolic process
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
axon guidance
glycosaminoglycan metabolic process
positive regulation of skeletal muscle cell differentiation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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