Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1) and Jurkat (Lane 2). The blots were probed with Anti-SOD1 Mouse Monoclonal Antibody (Product # MA1-105,0.5 µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Poly-HRP Secondary Antibody, HRP conjugate (Product # 32230) at dilutions 1:2,500 (Fig. 1), 1:5,000 (Fig. 2) and 1:10,000 (Fig. 3). A 18 kDa band corresponding to SOD1 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Goat / IgG|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with proprietary stabilizer|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:5,000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
This antibody may cross-react with immunoglobulins from other species.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cathepsin G-dependent modulation of platelet thrombus formation in vivo by blood neutrophils.
32230 was used in western blot to study the role of neutrophil cathepsin G in the regulation of platelet thrombus formation
|Faraday N,Schunke K,Saleem S,Fu J,Wang B,Zhang J,Morrell C,Dore S||PloS one (8:null)||2013|
Cross-talk between microRNAs, nuclear factor E2-related factor 2, and heme oxygenase-1 in ochratoxin A-induced toxic effects in renal proximal tubular epithelial cells.
32230 was used in western blot to study the roles of microRNAs, Nrf2 and heme oxygenase-1 in the nephrotoxic mechanism of ochratoxin-A
|Stachurska A,Ciesla M,Kozakowska M,Wolffram S,Boesch-Saadatmandi C,Rimbach G,Jozkowicz A,Dulak J,Loboda A||Molecular nutrition and food research (57:504)||2013|
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.
32230 was used in western blot to investigate how AID-mediated events are regulated through the cell cycle.
|Hasham MG,Snow KJ,Donghia NM,Branca JA,Lessard MD,Stavnezer J,Shopland LS,Mills KD||Journal of immunology (Baltimore, Md. : 1950) (189:2374)||2012|