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|Tested species reactivity||Tag|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||twelve amino acid peptide CYPYDVPDYASL.|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.2, with 0.1% BSA|
|Contains||5mM sodium azide|
|Storage Conditions||4° C, store in dark|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. An HA tag is frequently incorporated into recombinant proteins for a variety of purposes. An anti-HA antibody can then be used to detect the protein when doing studies with transfected cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
ATG16L1 phosphorylation is oppositely regulated by CSNK2/casein kinase 2 and PPP1/protein phosphatase 1 which determines the fate of cardiomyocytes during hypoxia/reoxygenation.
A-21287 was used in immunoprecipitation to investigate the regulatory mechanism and role of ATG16L1 phosphorylation in autophagy induction using cardiomyocytes.
|Song H,Pu J,Wang L,Wu L,Xiao J,Liu Q,Chen J,Zhang M,Liu Y,Ni M,Mo J,Zheng Y,Wan D,Cai X,Cao Y,Xiao W,Ye L,Tu E,Lin Z,Wen J,Lu X,He J,Peng Y,Su J,Zhang H,Zhao Y,Lin M,Zhang Z||Autophagy (11:1308)||2015|
HA; HA Epitope Tag; HA-tag; human influenza hemagglutinin