Invitrogen

HDAC1 Polyclonal Antibody, Alexa Fluor™ 488

Product Image Gallery

HDAC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HDAC-1 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC-1 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-860-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

HDAC1 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation, ChIP Assay (WB, ICC/IF, IP, ChIP) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot, Immunocytochemistry, Immunoprecipitation and ChIP Assay

HDAC1 Antibody in Immunohistochemistry (IHC) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Immunohistochemistry

HDAC1 Antibody in Western Blot (WB) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

HDAC1 Antibody in Western Blot, ChIP Assay (WB, ChIP) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot and ChIP Assay

HDAC1 Antibody in Immunoprecipitation, ChIP Assay (IP, ChIP) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Immunoprecipitation and ChIP Assay

HDAC1 Antibody in ChIP Assay (ChIP) — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in ChIP Assay

Product Details

PA1-860-A488

Applications

Tested Dilution

Publications

Western Blot (WB)

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

View 1 publication 1 publication

Immunoprecipitation (IP)

View 2 publications 2 publications

ChIP assay (ChIP)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1

Conjugate

Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2662019

Product Specific Information

PA1860A488 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.

The PA1860A488 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

HDAC1 and HDAC2 control the specification of neural crest cells into peripheral glia.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jacob C,Lötscher P,Engler S,Baggiolini A,Varum Tavares S,Brügger V,John N,Büchmann-Møller S,Snider PL,Conway SJ,Yamaguchi T,Matthias P,Sommer L,Mantei N,Suter U

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Immunofluorescence

Wed Apr 23 00:00:00 EDT 2014

SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.

Nature neuroscience

Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Aug 01 00:00:00 EDT 2013

SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.

Nature neuroscience

Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Aug 01 00:00:00 EDT 2013

SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.

Nature neuroscience

Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Aug 01 00:00:00 EDT 2013

SIRT1 collaborates with ATM and HDAC1 to maintain genomic stability in neurons.

Nature neuroscience

Dobbin MM,Madabhushi R,Pan L,Chen Y,Kim D,Gao J,Ahanonu B,Pao PC,Qiu Y,Zhao Y,Tsai LH

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Aug 01 00:00:00 EDT 2013

Histone Deacetylase Inhibitors and Mithramycin A Impact a Similar Neuroprotective Pathway at a Crossroad between Cancer and Neurodegeneration.

Pharmaceuticals (Basel, Switzerland)

Sleiman SF,Berlin J,Basso M,Karuppagounder SS,Rohr J,Ratan RR

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Mon Aug 22 00:00:00 EDT 2011

Age-dependent epigenetic control of differentiation inhibitors is critical for remyelination efficiency.

Nature neuroscience

Shen S,Sandoval J,Swiss VA,Li J,Dupree J,Franklin RJ,Casaccia-Bonnefil P

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in ChIP assay

Mon Sep 01 00:00:00 EDT 2008

Age-dependent epigenetic control of differentiation inhibitors is critical for remyelination efficiency.

Nature neuroscience

Shen S,Sandoval J,Swiss VA,Li J,Dupree J,Franklin RJ,Casaccia-Bonnefil P

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in ChIP assay

Mon Sep 01 00:00:00 EDT 2008

Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1.

Molecular and cellular biology

Duan Z,Zarebski A,Montoya-Durango D,Grimes HL,Horwitz M

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Dec 01 00:00:00 EST 2005

Gfi1 coordinates epigenetic repression of p21Cip/WAF1 by recruitment of histone lysine methyltransferase G9a and histone deacetylase 1.

Molecular and cellular biology

Duan Z,Zarebski A,Montoya-Durango D,Grimes HL,Horwitz M

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Western Blot

Thu Dec 01 00:00:00 EST 2005

Retinoblastoma protein transcriptional repression through histone deacetylation of a single nucleosome.

Molecular and cellular biology

Morrison AJ,Sardet C,Herrera RE

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in ChIP assay

Fri Feb 01 00:00:00 EST 2002

Retinoblastoma protein transcriptional repression through histone deacetylation of a single nucleosome.

Molecular and cellular biology

Morrison AJ,Sardet C,Herrera RE

Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in ChIP assay

Fri Feb 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; histone deacetylase 1-like protein; Protein deacetylase HDAC1; Protein decrotonylase HDAC1; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2

Gene Aliases: HDAC1; RPD3L1

HDAC1 Polyclonal Antibody, Alexa Fluor™ 488

Product Details

PA1-860-A488

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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