Invitrogen

HDAC1 Polyclonal Antibody, Alexa Fluor 488

12 Published Figures

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HDAC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HDAC-1 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a HDAC-1 Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-860-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488) in Immunofluorescence

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Figure 3 MTM affects class 1 HDAC expression levels. Real time RTPCR shows that MTM (300 nM) does not significantly affect HDAC1 ( A ) and HDAC3 ( C ) expression levels; whereas it significantly reduces HDAC2 ( B ) and HDAC8 ( D ) expression levels. These changes covary with neuroprotection by MTM as the non-protective analog PreB (300 nM) has no effect on the expression of these genes. Cells were treated for five hours and the experiments were repeated three times. ( E ): Western Blots show that MTM treatment reduces the protein levels of class 1 HDACs. ( F ): Quantification of three different western blots reveals that only HDAC2 and HDAC3 protein levels are significantly affected by MTM treatment. ( G ): Unlike sodium butyrate (5 mM) and TSA (0.66 muM), MTM does not induce acetylation of histone H3. For the western blot experiments, cells were treated with the compounds for 48 hours and experiments were repeated three times. Statistical analysis was conducted by 1way ANOVA followed by the Dunnett post test. Significant levels compared to control treatment alone. *: p < 0.05 and **: p < 0.01 and ***: p < 0.0001.

HDAC1 Antibody — Figure 4 MTM affects class 1 HDAC expression levels. ( A ) Western blot analysis reveals that Myc knockdown using ShRNA significantly affects class I HDAC protein levels. ( B ) Quantification of Western blots. Statistical analysis was conducted by 1way ANOVA followed by the Dunnett post test. Significant levels compared to control shRNA alone. * p < 0.05; and ** p < 0.01; and *** p < 0.0001.

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

HDAC1 Antibody — Published figure using HDAC1 polyclonal antibody (Product # PA1-860-A488)

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 3 publications 3 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunofluorescence (IF)

-

View 3 publications 3 publications

Immunoprecipitation (IP)

-

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

1-10 µg/mL

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Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1

Conjugate

Alexa Fluor® 488

Excitation/Emission Profile

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2662019

Product Specific Information

PA1860A488 detects histone deacetylase 1 (HDAC1) from human, mouse, rat, hamster and canine tissues and cells.

The PA1860A488 immunogen is a synthetic peptide corresponding to residues C E(467) E K P E A K G V K E E V K L A(482) of human HDAC1. This immunizing peptide (Cat. # PEP-069) is available for use in neutralization and control experiments.

Target Information

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. HDAC1 belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, HDAC1 deacetylates p53 and modulates its effect on cell growth and apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686H12203; HD1; Histone deacetylase 1; histone deacetylase 1-like protein; reduced potassium dependency, yeast homolog-like 1; RP4-811H24.2

Gene Aliases: GON-10; HD1; HDAC1; Hdac1-ps; MommeD5; RPD3; RPD3L1

UniProt ID: (Human) Q13547, (Mouse) O09106, (Rat) Q4QQW4

Entrez Gene ID: (Human) 3065, (Dog) 487309, (Mouse) 433759, (Rat) 297893, (Chinese hamster) 100752721

Molecular Function: deacetylase hydrolase nucleic acid binding oxidoreductase reductase

negative regulation of transcription from RNA polymerase II promoter chromatin remodeling methylation-dependent chromatin silencing transcription, DNA-templated protein deacetylation blood coagulation positive regulation of cell proliferation epidermal cell differentiation negative regulation of gene expression negative regulation of myotube differentiation positive regulation of receptor biosynthetic process viral process chromatin modification histone deacetylation circadian regulation of gene expression odontogenesis of dentin-containing tooth embryonic digit morphogenesis ATP-dependent chromatin remodeling negative regulation of apoptotic process negative regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of androgen receptor signaling pathway hair follicle placode formation eyelid development in camera-type eye fungiform papilla formation histone H3 deacetylation histone H4 deacetylation negative regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator beta-catenin-TCF complex assembly response to amphetamine regulation of transcription, DNA-templated transcription from RNA polymerase II promoter endoderm development circadian rhythm negative regulation of cell proliferation hippocampus development neuron differentiation positive regulation of interleukin-1 production positive regulation of tumor necrosis factor production cellular response to oxidative stress positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of neuron apoptotic process negative regulation of insulin secretion rhythmic process positive regulation of oligodendrocyte differentiation positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of type B pancreatic cell apoptotic process negative regulation of peptidyl-lysine acetylation negative regulation of intrinsic apoptotic signaling pathway response to caffeine response to lipopolysaccharide response to drug response to hyperoxia cellular response to tumor necrosis factor

Process(es)

transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding core promoter binding RNA polymerase II transcription factor binding RNA polymerase II repressing transcription factor binding RNA polymerase II transcription corepressor activity transcription factor activity, sequence-specific DNA binding histone deacetylase activity protein binding transcription factor binding deacetylase activity enzyme binding nucleosomal DNA binding NAD-dependent histone deacetylase activity (H3-K14 specific) protein deacetylase activity activating transcription factor binding histone deacetylase binding transcription regulatory region DNA binding protein N-terminus binding NF-kappaB binding repressing transcription factor binding DNA binding chromatin binding transcription corepressor activity hydrolase activity histone deacetylase activity (H3-K14 specific) histone deacetylase activity (H3-K9 specific) protein complex binding histone deacetylase activity (H4-K16 specific) Krueppel-associated box domain binding NAD-dependent histone deacetylase activity (H3-K9 specific) NAD-dependent histone deacetylase activity (H4-K16 specific) NAD-dependent histone deacetylase activity (H3-K18 specific)