Invitrogen

HK1 Polyclonal Antibody

Product Image Gallery

HK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence staining of HK1 in HeLa cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with HK1 Polyclonal Antibody (Product # PA5-117986, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

HK1 Antibody in Western Blot (WB) — Western Blot using HK1 Polyclonal Antibody (Product # PA5-117986) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 102 kDa. Observed band size: 115 kDa. (We are unsure of the identity of these extra bands).

HK1 Antibody in Immunoprecipitation (IP) — HK1 Immunoprecipitation using: Lane A: 0.5 mg Hela Whole Cell Lysate 0.5 µL with HK1 Polyclonal Antibody (Product # PA5-117986) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: HK1 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 102 kDa. Observed band size: 102 kDa.

Product Details

PA5-117986

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunocytochemistry (ICC/IF)	1:1,500-1:50,000	-
Immunoprecipitation (IP)	0.2-1 μL/mg of lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide corresponding to the center region of the Human HK1
Conjugate	Unconjugated
Form	Liquid
Purification	Antigen affinity chromatography, Protein A
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Target Information

This gene encodes a member of the ARHGAP family of Rho/Rac/Cdc42-like GTPase activating proteins. The encoded protein interacts with the Ras-related protein Gem through its N-terminal domain. Separately, it interacts with RhoA through a RhoGAP domain, and stimulates RhoA-dependent GTPase activity. Alternatively spliced variants that encode different protein isoforms have been described but their full-length nature has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.