Immunofluorescent analysis of MHC I HLA-A B C (green) showing staining in the cytoplasm and membrane of non-permeabilized Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an MHC I (HLA-A B C) monoclonal antibody (Product # MA5-11723) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Cell membranes of human tonsil lymphocytes|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 ug/test|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11723 targets MHC I (HLA-A B C) in IHC (F), ICC/IF, WB and FACS applications and shows reactivity with Human and Mouse samples.
The MA5-11723 immunogen is cell membranes of human tonsil lymphocytes.
Human MHC class I antigens (HLA-A, B and C) are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC- associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Hepatitis C virus infection in inclusion body myositis: A case-control study.
MA5-11723 was used in immunohistochemistry - paraffin section to determine if there is an association between inclusion body myositis and hepatitis C virus infection
|Uruha A,Noguchi S,Hayashi YK,Tsuburaya RS,Yonekawa T,Nonaka I,Nishino I||Neurology (86:211)||2016|
Uptake of Helicobacter pylori vesicles is facilitated by clathrin-dependent and clathrin-independent endocytic pathways.
MA5-11723 was used in immunocytochemistry to study the role of clathrin-dependent and clathrin-independent routes in the uptake of Helicobacter pylori vesicles by gastric epithelial cells
|Olofsson A,Nygård Skalman L,Obi I,Lundmark R,Arnqvist A||mBio (5:e00979)||2014|
Alterations in the Arf6-regulated plasma membrane endosomal recycling pathway in cells overexpressing the tetraspan protein Gas3/PMP22.
MA5-11723 was used in immunocytochemistry to study alterations in Arf6-regulated plasma membrane endosomal recycling in cells overexpressing Gas3/PMP22
|Chies R,Nobbio L,Edomi P,Schenone A,Schneider C,Brancolini C||Journal of cell science (116:987)||2003|
Co-existence of epithelioid and fibroblastoid subsets in a sarcomatoid renal carcinoma cell line revealed by clonal studies.
MA5-11723 was used in flow cytometry to study the presence in a sarcomatoid renal carcinoma cell line of both epithelioid and fibroblastoid cells
|Hsieh CH,Chen HC,Chang YH,Pang ST,Kuo ML,Chuang CK,Liao SK||Anticancer research (33:4875)||2013|
Induction of metastatic cancer stem cells from the NK/LAK-resistant floating, but not adherent, subset of the UP-LN1 carcinoma cell line by IFN-¿.
MA5-11723 was used in flow cytometry to study the induction of metastatic cancer stem cells from UP-LN1 cell line
|Chen HC,Chou AS,Liu YC,Hsieh CH,Kang CC,Pang ST,Yeh CT,Liu HP,Liao SK||Laboratory investigation; a journal of technical methods and pathology (91:1502)||2011|
|Not Applicable||Not Cited||
The human herpesvirus-7 (HHV-7) U21 immunoevasin subverts NK-mediated cytoxicity through modulation of MICA and MICB.
MA5-11723 was used in western blot to report that the human herpesvirus-7 U21 gene product interferes with natural killer cell recognition
|Schneider CL,Hudson AW||PLoS pathogens (7:null)||2011|
Establishment of a quantitative ELISA capable of determining peptide - MHC class I interaction.
MA5-11723 was used in ELISA to develop a quantitative ELISA assay for studying peptide-MHC class I interactions
|Sylvester-Hvid C,Kristensen N,Blicher T,Ferré H,Lauemøller SL,Wolf XA,Lamberth K,Nissen MH,Pedersen LØ,Buus S||Tissue antigens (59:251)||2002|