Immunohistochemical analysis of HOXA10 in paraffin-embedded human testis following steamed antigen retrieval with a citrate buffer, pH 6, using a HOXA10 polyclonal antibody (Product# PA5-37866) at a dilution of 5ug/ml. Detection was performed using an AP-conjugated secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence PQATSCSFAQNIKE, from the internal region of HOXA10|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Western Blot (WB)||0.2-0.6 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, pig and cow based on sequence homology.
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.