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HSP70/HSC70 Antibody (MA5-45094) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Hsp70 monoclonal antibody (Product # MA5-45094) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
Product Details
MA5-45094
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
C. elegans,
Chicken,
Fruit fly,
Fish,
Guinea pig,
Hamster,
Human,
Mammal,
Mouse,
Non-human primate,
Sheep,
Plant,
Pig,
Rabbit,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N27F3-4
Immunogen
Recombinant HSP70/HSC70
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9.1mM sodium bicarbonate/PBS, pH 7.2, with 581mM DMSO, 123mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931548
Product Specific Information
1 µg/mL of MA5-45094 was sufficient for detection of HSP70/HSC70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 72 (HSP) and approximately 73kDa (HSC).
Target Information
HSPA4 (Heat Shock Protein Family A (Hsp70) Member 4) is a Protein Coding gene. Diseases associated with HSPA4 include Chagas Disease and Babesiosis. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Cellular responses to stimuli. An important paralog of this gene is HSPH1. HSPA8 (Heat Shock Protein Family A (Hsp70) Member 8) is a Protein Coding gene. Diseases associated with HSPA8 include Auditory System Disease and Borna Disease. Among its related pathways are Processing of Capped Intron-Containing Pre-mRNA and Selective autophagy. Gene Ontology (GO) annotations related to this gene include RNA binding and ubiquitin protein ligase binding. An important paralog of this gene is HSPA2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: constitutive heat shock protein 70; epididymis luminal protein 33; epididymis secretory sperm binding protein Li 5a; epididymis secretory sperm binding protein Li 72p; Heat shock 70 kDa protein 1; Heat shock 70 kDa protein 4; Heat shock 70 kDa protein 8; Heat shock 70-related protein APG-2; heat shock 70kd protein 10; heat shock 70kd protein 10 (HSC71); heat shock 70kD protein 4; heat shock 70kD protein 8; heat shock 70kDa protein 1-like; heat shock 70kDa protein 4; heat shock 70kDa protein 8; heat shock cognate 70; heat shock cognate 71 kD protein; Heat shock cognate 71 kDa protein; heat shock cognate hsc73; heat shock cognate protein 54; Heat shock cognate protein 70; heat shock protein 8; heat shock protein A8; heat shock protein Apg-2; heat shock protein cognate 70; Heat shock protein family A member 8; Heat shock protein family H member 2; heat shock protein, 110 kDa; Hsc70 ATPase; hsp70 RY; HSP70RY; HSPA4/HSPA8; Ischemia responsive 94 kDa protein; LAP-1; Lipopolysaccharide-associated protein 1; LPS-associated protein 1; N-myristoyltransferase inhibitor protein 71; unnamed protein product
Gene Aliases: 2410008N15Rik; 70kDa; AI317151; APG-2; APG2; HEL-33; HEL-S-5a; HEL-S-72p; HS24/P52; HSC54; HSC70; hsc70.I; HSC71; Hsc73; Hsp110; HSP70; Hsp70RY; HSP71; HSP73; HSPA1; HSPA10; hspa1l; HSPA4; HSPA8; HSPH2; I79_004332; I79_022974; Irp94; LAP-1; LAP1; mKIAA4025; NIP71; RY; XELAEV_18037286mg; xhsp70
UniProt ID: (Dog) Q7YQC6, (Dog) Q2TFN9, (Human) P34932, (Human) P11142, (Chicken) O73885, (Bovine) P19120, (Rat) O88600, (Rat) P63018, (Mouse) P63017, (Chinese hamster) P19378
Entrez Gene ID: (Dog) 403612, (Chicken) 416339, (Dog) 474680, (Sheep) 101122782, (Human) 3308, (Pig) 100524853, (Bovine) 536558, (Rabbit) 100338049, (Dog) 479406, (Sheep) 101112844, (Human) 3312, (Chicken) 395853, (Bovine) 281831, (Rabbit) 100348873, (Pig) 100511890, (Mouse) 15525, (Rat) 266759, (Guinea pig) 100735343, (Chinese hamster) 100764628, (Guinea pig) 100713849, (Rat) 24468, (Mouse) 15481, (Chinese hamster) 100689472, (Western clawed frog) 100037899, (Xenopus) 379760
protein binding
ATP binding
G-protein coupled receptor binding
phosphatidylserine binding
ATPase activity
enzyme binding
MHC class II protein complex binding
heat shock protein binding
ubiquitin protein ligase binding
ATPase activity, coupled
poly(A) RNA binding
unfolded protein binding
C3HC4-type RING finger domain binding
cadherin binding involved in cell-cell adhesion
protein binding involved in protein folding
misfolded protein binding
clathrin-uncoating ATPase activity
nucleotide binding
RNA polymerase II transcription factor binding
protein complex binding
activating transcription factor binding
receptor binding
ADP binding
negative regulation of protein phosphorylation
response to heat
positive regulation of gene expression
negative regulation of gene expression
positive regulation of protein binding
negative regulation of apoptotic process
negative regulation of DNA binding
protein import into mitochondrial outer membrane
chaperone-mediated protein complex assembly
negative regulation of cell death
kidney development
positive regulation of angiogenesis
transcription, DNA-templated
mRNA processing
protein folding
RNA splicing
protein refolding
negative regulation of transcription, DNA-templated
positive regulation of mRNA splicing, via spliceosome
chaperone mediated protein folding requiring cofactor
regulation of cell cycle
chaperone-mediated protein folding
clathrin coat disassembly
negative regulation of fibril organization
mRNA splicing, via spliceosome
protein targeting to lysosome
response to unfolded protein
neurotransmitter secretion
cellular response to starvation
viral process
regulation of protein stability
regulation of protein complex assembly
regulation of mRNA stability
ATP metabolic process
regulation of protein complex stability
chaperone-mediated autophagy
late endosomal microautophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
chaperone-mediated protein transport involved in chaperone-mediated autophagy
cell-cell adhesion
regulation of cellular response to heat
regulation of protein import
chaperone-mediated autophagy translocation complex disassembly
regulation of transcription, DNA-templated
gastrulation
axo-dendritic transport
embryo development ending in birth or egg hatching
vesicle-mediated transport
cellular response to unfolded protein
embryonic organ development
slow axonal transport
synaptic vesicle uncoating
positive regulation of microtubule nucleation
regulation of mitotic spindle assembly
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