Invitrogen

IFN gamma Monoclonal Antibody (4S.B3), PerCP-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

68 Published Figures

63 References

View all (192) IFN gamma antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

IFN gamma Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 70

IFN gamma Antibody (45-7319-42) in Flow

Intracellular staining of normal human peripheral blood cells stimulated for 5 hours with PMA/Ionomycin in the presence of Monensin with Anti-Human CD4 APC (Product # 17-0049-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human IFN gamma PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

IFN gamma Antibody — Intracellular staining of stimulated human peripheral blood cells. As expected based on known expression patterns, IFN gamma clone 4S.B3 stains a subset of CD4+ T cells only after stimulation and does not stain CD19+ B cells regardless of stimulation. Details: Normal human peripheral blood cells were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation & Permeabilization Buffer Set and protocol followed by intracellular staining with CD19 (clone SJ25C1), CD4 (clone RPA-T4), and IFN gamma (clone 4S.B3). Cells in the CD19+ (blue histogram) or CD4+ (purple histogram) gates were used for analysis. {TM}

Product Details

45-7319-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 63 publications 63 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4S.B3

Conjugate

PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10718246

Product Specific Information

Description: The 4S.B3 monoclonal antibody reacts with interferon-gamma (IFN gamma). Human IFN gamma is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN gamma in synergy with other cytokines, such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma. Glycosylation of IFN gamma does not affect its biological activity.

Applications Reported: This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 4S.B3 antibody has been pre-titrated and tested by intracellular staining of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Antigen-Specific Intraocular Cytokine Responses Distinguish Ocular Tuberculosis From Undifferentiated Uveitis in Tuberculosis-Immunoreactive Patients.

American journal of ophthalmology

Alam K,Sharma G,Forrester JV,Basu S

45-7319-42 was used in Flow cytometry/Cell sorting to show that the intraocular polyfunctional cytokine response is stronger in undifferentiated TST+ uveitis than in OTB patients, likely representing an exaggerated anti-TB immune response rather than active infection.

Wed Feb 01 00:00:00 EST 2023

GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint.

Cell & bioscience

Guo D,Jin C,Gao Y,Lin H,Zhang L,Zhou Y,Yao J,Duan Y,Ren Y,Hui X,Ge Y,Yang R,Jiang W

45-7319-42 was used in Flow cytometry/Cell sorting to indicate that GPR116 receptor had a negatively effect on NK cell function and downregulation of GPR116 receptor in NKG2D-CAR-NK92 cells could enhance the antitumor activity, which provides a new idea to enhance the antitumor efficiency of CAR NK cell therapy.

Thu Mar 09 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

An Fc-Competent Anti-Human TIGIT Blocking Antibody Ociperlimab (BGB-A1217) Elicits Strong Immune Responses and Potent Anti-Tumor Efficacy in Pre-Clinical Models.

Frontiers in immunology

Chen X,Xue L,Ding X,Zhang J,Jiang L,Liu S,Hou H,Jiang B,Cheng L,Zhu Q,Zhang L,Zhou X,Ma J,Liu Q,Li Y,Ren Z,Jiang B,Song X,Song J,Jin W,Wei M,Shen Z,Liu X,Wang L,Li K,Zhang T

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon May 02 00:00:00 EDT 2022

Preventing Surgery-Induced NK Cell Dysfunction Using Anti-TGF-β Immunotherapeutics.

International journal of molecular sciences

Market M,Tennakoon G,Scaffidi M,Cook DP,Angka L,Ng J,Tanese de Souza C,Kennedy MA,Vanderhyden BC,Auer RC

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Nov 23 00:00:00 EST 2022

LINC01123 promotes immune escape by sponging miR-214-3p to regulate B7-H3 in head and neck squamous-cell carcinoma.

Cell death & disease

Li H,Yang Z,Yang X,Zhang F,Wang J,Wu Z,Wanyan C,Meng Q,Gao W,Yang X,Wei J

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Feb 03 00:00:00 EST 2022

NRF2/Itaconate Axis Regulates Metabolism and Inflammatory Properties of T Cells in Children with JIA.

Antioxidants (Basel, Switzerland)

Rajendiran A,Subramanyam SH,Klemm P,Jankowski V,van Loosdregt J,Vastert B,Vollbach K,Wagner N,Tenbrock K,Ohl K

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Dec 08 00:00:00 EST 2022

Ex vivo assays show human gamma-delta T cells specific for common allergens are Th1-polarized in allergic donors.

Cell reports methods

Yu ED,Wang E,Garrigan E,Sutherland A,Khalil N,Kearns K,Pham J,Schulten V,Peters B,Frazier A,Sette A,da Silva Antunes R

45-7319-42 was used in flow cytometry to support the existence of allergen-reactive γδ T cells and their potential use in rebalancing dysregulated Th2 responses in allergic diseases.

Mon Dec 19 00:00:00 EST 2022

A vaccine targeting mutant IDH1 in newly diagnosed glioma.

Nature

Platten M,Bunse L,Wick A,Bunse T,Le Cornet L,Harting I,Sahm F,Sanghvi K,Tan CL,Poschke I,Green E,Justesen S,Behrens GA,Breckwoldt MO,Freitag A,Rother LM,Schmitt A,Schnell O,Hense J,Misch M,Krex D,Stevanovic S,Tabatabai G,Steinbach JP,Bendszus M,von Deimling A,Schmitt M,Wick W

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhou C,Wei W,Ma J,Yang Y,Liang L,Zhang Y,Wang Z,Chen X,Huang L,Wang W,Wu S

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Apr 07 00:00:00 EDT 2021

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Suppression of 4.1R enhances the potency of NKG2D-CAR T cells against pancreatic carcinoma via activating ERK signaling pathway.

Oncogenesis

Gao Y,Lin H,Guo D,Cheng S,Zhou Y,Zhang L,Yao J,Farooq MA,Ajmal I,Duan Y,He C,Tao L,Wu S,Liu M,Jiang W

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Suppression of 4.1R enhances the potency of NKG2D-CAR T cells against pancreatic carcinoma via activating ERK signaling pathway.

Oncogenesis

Gao Y,Lin H,Guo D,Cheng S,Zhou Y,Zhang L,Yao J,Farooq MA,Ajmal I,Duan Y,He C,Tao L,Wu S,Liu M,Jiang W

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis.

Journal of immunology research

Lin Y,Dong Y,Gao Y,Shi R,Li Y,Zhou X,Liu W,Li G,Qi Y,Wu Y

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Sep 20 00:00:00 EDT 2021

Peritoneal carcinomatosis of colorectal cancer is characterized by structural and functional reorganization of the tumor microenvironment inducing senescence and proliferation arrest in cancer cells.

Oncoimmunology

Seebauer CT,Brunner S,Glockzin G,Piso P,Ruemmele P,Schlitt HJ,Geissler EK,Fichtner-Feigl S,Kesselring R

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

A protocol for isolation of primary human immune cells from the liver and mesenteric white adipose tissue biopsies.

STAR protocols

Moreno-Fernandez ME,Damen MSMA,Divanovic S

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Suppression of mucosal Th17 memory responses by acellular pertussis vaccines enhances nasal Bordetella pertussis carriage.

NPJ vaccines

Dubois V,Chatagnon J,Thiriard A,Bauderlique-Le Roy H,Debrie AS,Coutte L,Locht C

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Jan 08 00:00:00 EST 2021

YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.

JCI insight

Lin J,Tang J,Lin J,He Y,Yu Z,Jiang R,Yang B,Ou Q

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Nov 22 00:00:00 EST 2021

Lasting antibody and T cell responses to SARS-CoV-2 in COVID-19 patients three months after infection.

Nature communications

Jiang XL,Wang GL,Zhao XN,Yan FH,Yao L,Kou ZQ,Ji SX,Zhang XL,Li CB,Duan LJ,Li Y,Zhang YW,Duan Q,Wang TC,Li ET,Wei X,Wang QY,Wang XF,Sun WY,Gao YW,Kang DM,Zhang JY,Ma MJ

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2021

NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca²⁺ entry in T cells.

Cell reports

Wu B,Woo JS,Vila P,Jew M,Leung J,Sun Z,Srikanth S,Gwack Y

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer.

Journal of translational medicine

Meng J,Peng J,Feng J,Maurer J,Li X,Li Y,Yao S,Chu R,Pan X,Li J,Zhang T,Liu L,Zhang Q,Yuan Z,Bu H,Song K,Kong B

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Sep 28 00:00:00 EDT 2021

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

TIGIT⁺ TIM-3⁺ NK cells are correlated with NK cell exhaustion and disease progression in patients with hepatitis B virus‑related hepatocellular carcinoma.

Oncoimmunology

Yu L,Liu X,Wang X,Yan F,Wang P,Jiang Y,Du J,Yang Z

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

Identification of HLA-A2-Restricted Mutant Epitopes from Neoantigens of Esophageal Squamous Cell Carcinoma.

Vaccines

Wang Z,Ran L,Chen C,Shi R,Dong Y,Li Y,Zhou X,Qi Y,Zhu P,Gao Y,Wu Y

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Oct 01 00:00:00 EDT 2021

Inhibition of Cholesterol Esterification Enzyme Enhances the Potency of Human Chimeric Antigen Receptor T Cells against Pancreatic Carcinoma.

Molecular therapy oncolytics

Zhao L,Liu Y,Zhao F,Jin Y,Feng J,Geng R,Sun J,Kang L,Yu L,Wei Y

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Mar 27 00:00:00 EDT 2020

TIRC7 inhibits Th1 cells by upregulating the expression of CTLA‑4 and STAT3 in mice with acute graft‑versus‑host disease.

Oncology reports

Zhu F,Qiu T,Zhu S,Zhao K,Chen C,Qiao J,Pan B,Yan Z,Chen W,Liu Q,Wu Q,Cao J,Sang W,Zeng L,Sun H,Li Z,Xu K

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer.

Nature communications

Buqué A,Bloy N,Perez-Lanzón M,Iribarren K,Humeau J,Pol JG,Levesque S,Mondragon L,Yamazaki T,Sato A,Aranda F,Durand S,Boissonnas A,Fucikova J,Senovilla L,Enot D,Hensler M,Kremer M,Stoll G,Hu Y,Massa C,Formenti SC,Seliger B,Elemento O,Spisek R,André F,Zitvogel L,Delaloge S,Kroemer G,Galluzzi L

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Jul 30 00:00:00 EDT 2020

Duration of Cellular and Humoral Responses after Quadrivalent Human Papillomavirus Vaccination in Healthy Female Adults with or without Prior Type 16 and/or 18 Exposure.

Vaccines

Lai L,Ault K,Rouphael N,Beck A,Domjahn B,Xu Y,Anderson EJ,Cheng A,Nakamura A,Hoagland RJ,Kelley C,Edupuganti S,Mask K,Nesin M,Unger ER,Panicker G,David H,Mulligan MJ

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Jun 30 00:00:00 EDT 2020

Single-cell transcriptomics identifies an effectorness gradient shaping the response of CD4⁺ T cells to cytokines.

Nature communications

Cano-Gamez E,Soskic B,Roumeliotis TI,So E,Smyth DJ,Baldrighi M,Willé D,Nakic N,Esparza-Gordillo J,Larminie CGC,Bronson PG,Tough DF,Rowan WC,Choudhary JS,Trynka G

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Apr 14 00:00:00 EDT 2020

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

A Method of Assessment of Human Natural Killer Cell Phenotype and Function in Whole Blood.

Frontiers in immunology

Market M,Tennakoon G,Ng J,Scaffidi M,de Souza CT,Kennedy MA,Auer RC

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Mar 29 00:00:00 EDT 2021

Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer.

Cell reports. Medicine

Li S,Simoni Y,Becht E,Loh CY,Li N,Lachance D,Koo SL,Lim TP,Tan EKW,Mathew R,Nguyen A,Golovato J,Berkson JD,Prlic M,Lee B,Minot SS,Nagarajan N,Dey N,Tan DSW,Tan IB,Newell EW

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

NPM1 upregulates the transcription of PD-L1 and suppresses T cell activity in triple-negative breast cancer.

Nature communications

Qin G,Wang X,Ye S,Li Y,Chen M,Wang S,Qin T,Zhang C,Li Y,Long Q,Hu H,Shi D,Li J,Zhang K,Zhai Q,Tang Y,Kang T,Lan P,Xie F,Lu J,Deng W

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces T_H2/T_H17 polarization through dectin-2.

The Journal of allergy and clinical immunology

Liang Y,Yu B,Chen J,Wu H,Xu Y,Yang B,Lu Q

45-7319 was used in Flow cytometry/Cell sorting to investigate epigenetic mechanisms underlying FCER1G demethylation and upregulation of FcRγ-related receptors on APCs and the consequent effect on allergic responses.

Tue Oct 01 00:00:00 EDT 2019

Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.

Nature communications

Zou F,Lu L,Liu J,Xia B,Zhang W,Hu Q,Liu W,Zhang Y,Lin Y,Jing S,Huang M,Huang B,Liu B,Zhang H

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Sep 11 00:00:00 EDT 2019

Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.

The Journal of clinical investigation

Kuranda K,Jean-Alphonse P,Leborgne C,Hardet R,Collaud F,Marmier S,Costa Verdera H,Ronzitti G,Veron P,Mingozzi F

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.

The Journal of clinical investigation

Kuranda K,Jean-Alphonse P,Leborgne C,Hardet R,Collaud F,Marmier S,Costa Verdera H,Ronzitti G,Veron P,Mingozzi F

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

RKIP mediates autoimmune inflammation by positively regulating IL-17R signaling.

EMBO reports

Lin W,Wang N,Zhou K,Su F,Jiang Y,Shou J,Liu H,Ma C,Qian Y,Wang K,Wang X

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.

European journal of immunology

Affandi AJ,Silva-Cardoso SC,Garcia S,Leijten EFA,van Kempen TS,Marut W,van Roon JAG,Radstake TRDJ

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.

European journal of immunology

Affandi AJ,Silva-Cardoso SC,Garcia S,Leijten EFA,van Kempen TS,Marut W,van Roon JAG,Radstake TRDJ

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

Human in vivo-generated monocyte-derived dendritic cells and macrophages cross-present antigens through a vacuolar pathway.

Nature communications

Tang-Huau TL,Gueguen P,Goudot C,Durand M,Bohec M,Baulande S,Pasquier B,Amigorena S,Segura E

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Jul 02 00:00:00 EDT 2018

Differential Reliance on Lipid Metabolism as a Salvage Pathway Underlies Functional Differences of T Cell Subsets in Poor Nutrient Environments.

Cell reports

Ecker C,Guo L,Voicu S,Gil-de-Gómez L,Medvec A,Cortina L,Pajda J,Andolina M,Torres-Castillo M,Donato JL,Mansour S,Zynda ER,Lin PY,Varela-Rohena A,Blair IA,Riley JL

45-7319 was used in Flow cytometry/Cell sorting to suggest effector memory T cells have limited ability to synthesize and metabolize fatty acids, allowing them to maintain function in nutrient-depleted microenvironments.

Tue Apr 17 00:00:00 EDT 2018

Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70.

Nature communications

Zhao M,Svensson MND,Venken K,Chawla A,Liang S,Engel I,Mydel P,Day J,Elewaut D,Bottini N,Kronenberg M

45-7319 was used in Flow cytometry/Cell sorting to study the effects of TCR signal strength during thymic differentiation on both IFN-γ production and the protective function of invariant natural killer T cells in arthritis.

Fri Jul 06 00:00:00 EDT 2018

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection.

PLoS pathogens

Iampietro M,Younan P,Nishida A,Dutta M,Lubaki NM,Santos RI,Koup RA,Katze MG,Bukreyev A

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon May 01 00:00:00 EDT 2017

Dominant immunosuppression of dendritic cell function by prostate-cancer-derived exosomes.

Journal of extracellular vesicles

Salimu J,Webber J,Gurney M,Al-Taei S,Clayton A,Tabi Z

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2017

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

Multifactorial T-cell hypofunction that is reversible can limit the efficacy of chimeric antigen receptor-transduced human T cells in solid tumors.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moon EK,Wang LC,Dolfi DV,Wilson CB,Ranganathan R,Sun J,Kapoor V,Scholler J,Puré E,Milone MC,June CH,Riley JL,Wherry EJ,Albelda SM

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Aug 15 00:00:00 EDT 2014

Failure to upregulate cell surface PD-1 is associated with dysregulated stimulation of T cells by TGN1412-like CD28 superagonist.

mAbs

Thaventhiran T,Alhumeed N,Yeang HX,Sethu S,Downey JS,Alghanem AF,Olayanju A,Smith EL,Cross MJ,Webb SD,Williams DP,Bristow A,Ball C,Stebbings R,Sathish JG

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Aug 24 00:00:00 EDT 2015

Transcriptional profile of tuberculosis antigen-specific T cells reveals novel multifunctional features.

Journal of immunology (Baltimore, Md. : 1950)

Arlehamn CL,Seumois G,Gerasimova A,Huang C,Fu Z,Yue X,Sette A,Vijayanand P,Peters B

45-7319 was used in Flow cytometry/Cell sorting to study the role of CD4(+) T cells in latent tuberculosis infection, showing that tuberculosis antigen-specific T cells have novel multifunctional features.

Mon Sep 15 00:00:00 EDT 2014

Tumor-derived exosomes promote tumor progression and T-cell dysfunction through the regulation of enriched exosomal microRNAs in human nasopharyngeal carcinoma.

Oncotarget

Ye SB,Li ZL,Luo DH,Huang BJ,Chen YS,Zhang XS,Cui J,Zeng YX,Li J

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Wed Jul 30 00:00:00 EDT 2014

T helper cell subsets specific for Pseudomonas aeruginosa in healthy individuals and patients with cystic fibrosis.

PloS one

Bayes HK,Bicknell S,MacGregor G,Evans TJ

45-7319 was used in Flow cytometry/Cell sorting to compare the T helper cells resonses to Pseudomonas aeruginosa infection in both healthy and cystic-fibrosis suffering individuals.

Wed Oct 29 00:00:00 EDT 2014

Elevated frequencies of circulating Th22 cell in addition to Th17 cell and Th17/Th1 cell in patients with acute coronary syndrome.

PloS one

Zhang L,Wang T,Wang XQ,Du RZ,Zhang KN,Liu XG,Ma DX,Yu S,Su GH,Li ZH,Guan YQ,Du NL

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Mon Aug 18 00:00:00 EDT 2014

Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer.

The Journal of clinical endocrinology and metabolism

French JD,Kotnis GR,Said S,Raeburn CD,McIntyre RC,Klopper JP,Haugen BR

45-7319 was used in Flow cytometry/Cell sorting to characterise the CD4(+) T cell response to metastatic PTC.

Fri Jun 01 00:00:00 EDT 2012

Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

PloS one

Zhang L,Li YG,Li YH,Qi L,Liu XG,Yuan CZ,Hu NW,Ma DX,Li ZF,Yang Q,Li W,Li JM

Published figure using IFN gamma monoclonal antibody (Product # 45-7319-42) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g

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Gene Aliases: IFG; IFI; IFNG

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UniProt ID: (Human) P01579

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Entrez Gene ID: (Human) 3458

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Molecular Function: cytokine interferon superfamily intercellular signal molecule

Function(s)

Process(es)

cytokine activity interferon-gamma receptor binding

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IFN gamma Monoclonal Antibody (4S.B3), PerCP-Cyanine5.5, eBioscience™

IFN gamma Antibody (45-7319-42) in Flow

Product Details

45-7319-42

Flow Cytometry (Flow)

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