|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS with 4mg/ml BSA, sucrose|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
IFN gamma, also called Type II interferon, macrophage activation factor, and immune interferon is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE it appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFNg is involved in cytokine regulation and also acts synergistically with other cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells.
MHCIFG04 was used in flow cytometry to study GATA-3 and T-box expression in human T cells
|De Fanis U,Mori F,Kurnat RJ,Lee WK,Bova M,Adkinson NF,Casolaro V||Blood (109:4343)||2007|
|Not Applicable||Not Cited||
Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood.
MHCIFG04 was used in flow cytometry to use flow cytometry to study granzyme A, B, and K expression in human peripheral blood lymphocytes
|Bratke K,Kuepper M,Bade B,Virchow JC,Luttmann W||European journal of immunology (35:2608)||2005|
Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations.
MHCIFG04 was used in flow cytometry to report the adaptive T cell responses to Japanese encephalitis virus in a naturally exposed healthy virus-immune human cohort.
|Kumar P,Uchil PD,Sulochana P,Nirmala G,Chandrashekar R,Haridattatreya M,Satchidanandam V||Archives of virology (148:1569)||2003|
Patterns of A2A extracellular adenosine receptor expression in different functional subsets of human peripheral T cells. Flow cytometry studies with anti-A2A receptor monoclonal antibodies.
MHCIFG04 was used in flow cytometry to elucidate the role of A2ARs in the regulation of immune response.
|Koshiba M,Rosin DL,Hayashi N,Linden J,Sitkovsky MV||Molecular pharmacology (55:614)||1999|