M421B targets IL-1 beta in ELISA, and WB applications and shows reactivity with Human samples.
A precipitate may be present in the solution. Mix for at least five seconds prior to use.
M421B detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.
The M421B IL1b antibody (clone ILB1-H67) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420BB (biotinylated conjugate of clone ILB1-H6). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.
Antibody M421B (clone ILB1-H67) and biotinylated antibody M420BB (clone ILB1-H6) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).
Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Catabolin; IL-1 beta; IL-1beta; interleukin 1, beta; Interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Gene Aliases: IL-1; IL1-BETA; IL1B; IL1F2
UniProt ID: (Human) P01584
Entrez Gene ID: (Human) 3553