|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|ELISA (ELISA)||See 8 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human IL-1 beta|
M421B targets IL-1 beta in ELISA, and WB applications and shows reactivity with Human samples.
A precipitate may be present in the solution. Mix for at least five seconds prior to use.
M421B detects IL-1 beta which has a predicted molecular weight of approximately 31 kDa.
The M421B IL1b antibody (clone ILB1-H67) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M420BB (biotinylated conjugate of clone ILB1-H6). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody M421B (clone ILB1-H67) and biotinylated antibody M420BB (clone ILB1-H6) have successfully been used in combination with recombinant IL1 beta protein SIL1B in ELISA applications.
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Catabolin; IL-1 beta; IL-1beta; interleukin 1, beta; Interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Gene Aliases: IL-1; IL1-BETA; IL1B; IL1F2
UniProt ID: (Human) P01584
Entrez Gene ID: (Human) 3553
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