Invitrogen

IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

25 References

View all (63) IL-8 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

IL-8 (CXCL8) Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of IL-8 was performed using 70% confluent log phase U-87 MG cells treated 1 uM thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IL-8 (3IL8-H10) Mouse Monoclonal Antibody (Product # M801) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

IL-8 (CXCL8) Antibody in ELISA (ELISA) — Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 µL per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/mL across a 3 µg/mL mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 µL per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at a 1:4,000 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

IL-8 (CXCL8) Antibody in Radioimmune assays (RIA) — Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in Radioimmune assays

IL-8 (CXCL8) Antibody — Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. Immunofluorescence analysis of CXCL8 using anti-CXCL8 mouse monoclonal antibody (Catalog # M801) shows increased expression of CXCL8 in U87MG upon treatment with thapsigargin. {TM}

Product Details

M801

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	2 µg/mL	View 2 publications 2 publications
ELISA (ELISA)	Assay-dependent	View 21 publications 21 publications
Radioimmune Assays (RIA)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	3IL8-H10
Immunogen	Recombinant human IL-8
Conjugate	Unconjugated
Form	Liquid
Concentration	1.0 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	no preservative
Storage conditions	-20°C
RRID	AB_223583

Product Specific Information

M801 targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M801 immunogen is recombinant human IL-8.

M801 detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M801 IL8 antibody (clone 3IL8-H10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M801 (clone 3IL8-H10) and biotinylated antibody M802B (clone I8-S2) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

Interleukin 8 (IL-8, CXCL8) is a 72 amino acid pro-inflammatory factor which belongs to the CXC subfamily of chemokines, and are bound by the cell surface receptors IL-8RA and IL-8RB. IL-8 functions as a chemoattractant and potent angiogenic factor. The expression and secretion of IL-8 can be induced by diverse inflammatory stimuli in many cells, including macrophages and endothelial cells. In endothelial cells, IL-8 is present in storage vesicles called Weibel-Palade bodies. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1 (NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. IL-8 and ten other members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours, and IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Impact of Progerin Expression on Adipogenesis in Hutchinson-Gilford Progeria Skin-Derived Precursor Cells.

Cells

Najdi F,Krüger P,Djabali K

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in Immunocytochemistry

Fri Jun 25 00:00:00 EDT 2021

Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer.

Journal of cellular and molecular medicine

Chen C,Zhang R,Ma L,Li Q,Zhao YL,Zhang GJ,Zhang D,Li WZ,Cao S,Wang L,Geng ZM

M801 was used in an ELISA assay to demonstrate how Neuropilin-1 causes gallbladder cancer cell proliferation through upregulation by cancer-associated fibroblast-secreted IL-8.

Sun Nov 01 00:00:00 EDT 2020

Neuropilin-1 is up-regulated by cancer-associated fibroblast-secreted IL-8 and associated with cell proliferation of gallbladder cancer.

Journal of cellular and molecular medicine

Chen C,Zhang R,Ma L,Li Q,Zhao YL,Zhang GJ,Zhang D,Li WZ,Cao S,Wang L,Geng ZM

M801 was used in an ELISA assay to demonstrate how Neuropilin-1 causes gallbladder cancer cell proliferation through upregulation by cancer-associated fibroblast-secreted IL-8.

Sun Nov 01 00:00:00 EDT 2020

Extravascular gelation shrinkage-derived internal stress enables tumor starvation therapy with suppressed metastasis and recurrence.

Nature communications

Zhang K,Fang Y,He Y,Yin H,Guan X,Pu Y,Zhou B,Yue W,Ren W,Du D,Li H,Liu C,Sun L,Chen Y,Xu H

M801 was used in an ELISA assay to establish an extravascular gelation shrinkage-derived internal stress strategy for squeezing and narrowing blood vessels, occluding blood & nutrition supply, reducing vascular density, inducing hypoxia and apoptosis and eventually realizing starvation therapy of malignancies.

Tue Nov 26 00:00:00 EST 2019

Combined effects of okadaic acid and pectenotoxin-2, 13-desmethylspirolide C or yessotoxin in human intestinal Caco-2 cells.

Chemosphere

Alarcan J,Barbé S,Kopp B,Hessel-Pras S,Braeuning A,Lampen A,Le Hégarat L,Fessard V

M801 was used in ELISA to examine the effects of three binary mixtures, OA/PTX-2, OA/SPX-1 and OA/YTX on human intestinal Caco-2 cells.

Thu Aug 01 00:00:00 EDT 2019

The Antiseptic Octenidine Inhibits Langerhans Cell Activation and Modulates Cytokine Expression upon Superficial Wounding with Tape Stripping.

Journal of immunology research

Nikolić N,Kienzl P,Tajpara P,Vierhapper M,Matiasek J,Elbe-Bürger A

M801 was used in an ELISA assay to investigate the influence of octenidine on epidermal Langerhans cells and cytokine secretion pattern of skin cells during wound healing in a model without disruption of the normal skin structure.

Thu Jul 18 00:00:00 EDT 2019

Resolvin D2 decreases TLR4 expression to mediate resolution in human monocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Croasdell A,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Thu Sep 01 00:00:00 EDT 2016

Microparticles engineered to highly express peroxisome proliferator-activated receptor-γ decreased inflammatory mediator production and increased adhesion of recipient monocytes.

PloS one

Sahler J,Woeller CF,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Mon Jul 13 00:00:00 EDT 2015

The human salivary proteome is radiation responsive.

Radiation research

Moore HD,Ivey RG,Voytovich UJ,Lin C,Stirewalt DL,Pogosova-Agadjanyan EL,Paulovich AG

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in Radioimmune Assays

Thu May 01 00:00:00 EDT 2014

Attenuation of inflammatory mediator production by the NF-κB member RelB is mediated by microRNA-146a in lung fibroblasts.

American journal of physiology. Lung cellular and molecular physiology

McMillan DH,Woeller CF,Thatcher TH,Spinelli SL,Maggirwar SB,Sime PJ,Phipps RP

Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801) in ELISA

Sat Jun 01 00:00:00 EDT 2013

Transforming growth factor-β impairs glucocorticoid activity in the A549 lung adenocarcinoma cell line.

British journal of pharmacology

Salem S,Harris T,Mok JS,Li MY,Keenan CR,Schuliga MJ,Stewart AG

M801 was used in ELISA to investigate the effect of transforming growth factor-beta on glucocorticoid activity in human A549 cells

Wed Aug 01 00:00:00 EDT 2012

Ocular fibroblast diversity: implications for inflammation and ocular wound healing.

Investigative ophthalmology & visual science

Xi X,McMillan DH,Lehmann GM,Sime PJ,Libby RT,Huxlin KR,Feldon SE,Phipps RP

M801 was used in ELISA to study the significance of ocular fibroblast diversity for inflammation and ocular wound healing

Fri Jul 01 00:00:00 EDT 2011

Inhibitors of p38 suppress cytokine production in rheumatoid arthritis synovial membranes: does variable inhibition of interleukin-6 production limit effectiveness in vivo?

Arthritis and rheumatism

Page TH,Brown A,Timms EM,Foxwell BM,Ray KP

M801 was used in ELISA to investigate the role of p38 kinase in rheumatoid arthritis synovial membranes

Mon Nov 01 00:00:00 EDT 2010

Intermittent haemodiafiltration in refractory congestive heart failure: BNP and balance of inflammatory cytokines.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Libetta C,Sepe V,Zucchi M,Pisacco P,Cosmai L,Meloni F,Campana C,Rampino T,Monti C,Tavazzi L,Dal Canton A

M801 was used in ELISA to assess the interleukin-8 level in patients with decompensated congestive heart failure

Sun Jul 01 00:00:00 EDT 2007

IgE modulates neutrophil survival in asthma: role of mitochondrial pathway.

Journal of immunology (Baltimore, Md. : 1950)

Saffar AS,Alphonse MP,Shan L,Hayglass KT,Simons FE,Gounni AS

M801 was used in ELISA to study the role of mitochondrial pathway in neutrophil survival

Thu Feb 15 00:00:00 EST 2007

Protein microarray platform for the multiplex analysis of biomarkers in human sera.

Journal of immunological methods

Urbanowska T,Mangialaio S,Zickler C,Cheevapruk S,Hasler P,Regenass S,Legay F

M801 was used in ELISA to develop a protein microarray platform for the multiplex analysis of biomarkers in human sera

Fri Oct 20 00:00:00 EDT 2006

Expression of a Porphyromonas gingivalis lipid A palmitylacyltransferase in Escherichia coli yields a chimeric lipid A with altered ability to stimulate interleukin-8 secretion.

Cellular microbiology

Bainbridge BW,Coats SR,Pham TT,Reife RA,Darveau RP

M801 was used in an ELISA assay to study the cloning, expression and characterisation of a Porphyromonas gingivalis htrB homolog.

Sun Jan 01 00:00:00 EST 2006

Oxidized ATP (oATP) attenuates proinflammatory signaling via P2 receptor-independent mechanisms.

British journal of pharmacology

Beigi RD,Kertesy SB,Aquilina G,Dubyak GR

M801 was used in ELISA to study the role of oxidized ATP in proinflammatory signaling

Wed Oct 01 00:00:00 EDT 2003

Development of protein microarray technology to monitor biomarkers of rheumatoid arthritis disease.

Cell biology and toxicology

Urbanowska T,Mangialaio S,Hartmann C,Legay F

M801 was used in ELISA to develop protein microarray technology to monitor biomarkers of rheumatoid arthritis disease

Sun Jun 01 00:00:00 EDT 2003

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Transplantation proceedings

Meloni F,Cascina A,Paschetto E,Marone Bianco A,Morosini M,Pellegrini C,Fietta A,Vitulo P,Pozzi E,Viganò M

M801 was used in ELISA to investigate the effect of immunosuppressive regimens on cytokine expression in lung transplanted patients

Sun Jun 01 00:00:00 EDT 2003

Regulation of interleukin 8 expression in human malignant melanoma cells.

Cancer research

Singh RK,Varney ML

M801 was used in ELISA to investigate the regulation of interleukin 8 expression in melanoma cells

Wed Apr 01 00:00:00 EST 1998

Antineutrophil cytoplasmic antibodies induce monocyte IL-8 release. Role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors.

The Journal of clinical investigation

Ralston DR,Marsh CB,Lowe MP,Wewers MD

M801 was used in ELISA to investigate the role of surface proteinase-3, alpha1-antitrypsin, and Fcgamma receptors in monocyte interleukin 8 release

Mon Sep 15 00:00:00 EDT 1997

Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.

The Journal of clinical investigation

Rasmussen SJ,Eckmann L,Quayle AJ,Shen L,Zhang YX,Anderson DJ,Fierer J,Stephens RS,Kagnoff MF

M801 was used in ELISA to investigate the important roles of epithelial cells in inflammatory response to chlamydia

Wed Jan 01 00:00:00 EST 1997

Monocyte Fc gamma receptor cross-linking induces IL-8 production.

Journal of immunology (Baltimore, Md. : 1950)

Marsh CB,Gadek JE,Kindt GC,Moore SA,Wewers MD

M801 was used in ELISA to investigate the influence of monocyte Fc gamma receptor cross-linking on the expression of interleukin 8

Fri Sep 15 00:00:00 EDT 1995

Rapamycin treatment depresses intragraft expression of KC/MIP-2, granzyme B, and IFN-gamma in rat recipients of cardiac allografts.

Journal of immunology (Baltimore, Md. : 1950)

Wieder KJ,Hancock WW,Schmidbauer G,Corpier CL,Wieder I,Kobzik L,Strom TB,Kupiec-Weglinski JW

M801 was used in immunohistochemistry to investigate the effects of rapamycin on KC/MIP-2, granzyme B, and interferon gamma expression on cardiac allografts

Thu Jul 15 00:00:00 EDT 1993

Bioinformatics

Protein Aliases: (Ala-IL-8)77; (Ser-IL-8)72; alveolar macrophage chemotactic factor I; ANX7; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; Granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; ILN; Interleukin; interleukin 8; Interleukin-8; Interleukin8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; RP11-537A6.8; small inducible cytokine subfamily B, member 8; SNX; SYNEXIN; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

UniProt ID: (Human) P10145

Entrez Gene ID: (Human) 3576

Molecular Function: chemokine