Invitrogen

IL-8 (CXCL8) Monoclonal Antibody (3IL8-H10)

Advanced Verification

This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details

7 Published Figures

18 References

View all (32) IL-8 antibodies

Datasheet

Datasheet

Product Image Gallery

IL-8 (CXCL8) Antibody — Published figure using IL-8 (CXCL8) monoclonal antibody (Product # M801)

IL-8 (CXCL8) Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of IL-8 was performed using 70% confluent log phase U-87 MG cells treated 1 uM thapsigargin for 24 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with IL-8 (3IL8-H10) Mouse Monoclonal Antibody (Product # M801) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

IL-8 (CXCL8) Antibody in ELISA (ELISA) — Sandwich ELISA analysis of human IL-8 was performed using a Human IL-8 Colorimetric ELISA kit (Product # EH2IL8) by loading 50 µL per well of Human IL-8 Recombinant Protein (Product # SIL8) in dodecuplicate at 1000, 400, 160, 64, 25.6, and 0 pg/mL across a 3 µg/mL mouse anti-Human IL-8 (Product # M801) pre-coated plate and incubating for 1 hour at room temperature. The plate was washed, and then incubated with 50 µL per well of a biotinylated mouse anti-human IL-8 monoclonal antibody (Product # M802B) in duplicate at 0.25 µg/mL for 1 hour at room temperature. The plate was washed and incubated with 100 µL per well of Streptavidin-HRP (Product # N504) in all test wells at a 1:4,000 dilution for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 30 minutes at room temperature in the dark. The plate was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.

IL-8 (CXCL8) Antibody in Cell Treatment — Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. Immunofluorescence analysis of CXCL8 using anti-CXCL8 mouse monoclonal antibody (Catalog # M801) shows increased expression of CXCL8 in U87MG upon treatment with thapsigargin. {TM}

Product Details

Tested Applications

Dilution

ELISA (ELISA)

Assay Dependent

Immunocytochemistry (ICC)

2 µg/mL

Immunofluorescence (IF)

2 µg/mL

Western Blot (WB)

Assay Dependent

Published Applications

ELISA (ELISA)

See 16 publications below

Radioimmune Assays (RIA)

See 1 publication below

Immunohistochemistry (IHC)

See 1 publication below

Product Specifications

Species Reactivity

Human

Published species

Human

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3IL8-H10

Immunogen

Recombinant human IL-8

Conjugate

Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

RRID

AB_223583

Product Specific Information

M801 targets IL-8 in ELISA, and WB applications and shows reactivity with Human samples.

The M801 immunogen is recombinant human IL-8.

M801 detects IL-8 which has a predicted molecular weight of approximately 9 kDa.

The M801 IL8 antibody (clone 3IL8-H10) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M802B (biotinylated conjugate of clone I8-S2). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody M801 (clone 3IL8-H10) and biotinylated antibody M802B (clone I8-S2) have successfully been used in combination with recombinant IL8 protein SIL8 in ELISA applications.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: (Ala-IL-8)77; (Ser-IL-8)72; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; GCP-1; GCP/IL-8 protein I; GCP/IL-8 protein II; GCP/IL-8 protein III; GCP/IL-8 protein IV; GCP/IL-8 protein V; GCP/IL-8 protein VI; Granulocyte chemotactic protein 1; IL-8; IL-8(1-77); IL-8(5-77); IL-8(6-77); IL-8(7-77); IL-8(8-77); IL-8(9-77); IL8/NAP1 form I; IL8/NAP1 form II; IL8/NAP1 form III; IL8/NAP1 form IV; IL8/NAP1 form V; IL8/NAP1 form VI; interleukin 8; Interleukin-8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; Lymphocyte-derived neutrophil-activating factor; LYNAP; MDNCF; MDNCF-a; MDNCF-b; MDNCF-c; MONAP; Monocyte-derived neutrophil chemotactic factor; Monocyte-derived neutrophil-activating peptide; NAF; NAP-1; Neutrophil-activating factor; neutrophil-activating peptide 1; Neutrophil-activating protein 1; Protein 3-10C; small inducible cytokine subfamily B, member 8; T cell chemotactic factor; T-cell chemotactic factor; tumor necrosis factor-induced gene 1

Gene Aliases: CXCL8; GCP-1; GCP1; IL8; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP-1; NAP1

UniProt ID: (Human) P10145

Entrez Gene ID: (Human) 3576

Molecular Function: chemokine cytokine signaling molecule