Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
FIGURE: 1 / 3
AA Sequence of recombinant protein: SMCKPITGTI NDLNQQVWTL QGQNLVAVPR SDSVTPVTVA VITCKYPEAL EQGRGDPIYL GIQNPEMCLY CEKVGEQPTL QLKEQKIMDL YGQPEPVKPF LFYRAKTGRT STLESVAFPD WFIASSKRDQ PIILTSELGK SYNTAFELNI ND.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-36gamma (IL-1F9). Anti-Human IL-36gamma (IL-1F9)-specific antibody was purified by affinity chromatography employing an immobilized Human IL-36gamma (IL-1F9) matrix.
Sandwich ELISA: To detect Human IL-36gamma (IL-1F9) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human IL-36gamma (IL-1F9) (500-P316Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-36gamma (IL-1F9).
Western Blot: To detect Human IL-36gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human IL-36gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous proinflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in proinflammatory responses during particular neutrophilic airway inflammation: activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: IL-1 epsilon; IL-1 related protein 2; IL-1(EPSILON); IL-1-epsilon; IL-1-related protein 2; IL-1F9; IL-1H1; IL-1RP2; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin-1 epsilon; Interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36; Interleukin-36 gamma
Gene Aliases: IL-1F9; IL-1H1; IL-1RP2; IL1E; IL1F9; IL1H1; IL1RP2; IL36G; UNQ2456/PRO5737
UniProt ID: (Human) Q9NZH8
Entrez Gene ID: (Human) 56300
Molecular Function: interleukin superfamily
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn moreGet expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support