Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:100 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100 | - |
Flow Cytometry (Flow) |
1:230 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Monoclonal |
Type |
Antibody |
Clone |
SP114 |
Immunogen |
A synthetic peptide near C-terminus of human MUM1/IRF4 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.102 mg/mL |
Purification |
Protein A/G |
Storage buffer |
PBS, pH 7.6, with 1% BSA |
Contains |
0.1% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2537901 |
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin's lymphoma.
The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5
Gene Aliases: IRF4; LSIRF; MUM1; NF-EM5; SHEP8
UniProt ID: (Human) Q15306
Entrez Gene ID: (Human) 3662
Molecular Function:
winged helix/forkhead transcription factor
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