Invitrogen

IRF4 Monoclonal Antibody (SP114)

Product Image Gallery

IRF4 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of MUM1/IRF4 using anti-MUM1/IRF4 Monoclonal Antibody (Product # MA5-16382) in Hodgkin's Lymphoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

IRF4 Antibody in Western Blot (WB) — Western blot analysis of Ramos Cells using anti-MUM1/IRF4 Monoclonal Antibody (Product # MA5-16382). The recommened dilution for this antibody in western blot applications is 1:100.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1:230

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Western Blot (WB)

1:100

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP114

Immunogen

A synthetic peptide near C-terminus of human MUM1/IRF4

Conjugate

Unconjugated

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS, pH 7.5, with 1% BSA

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2537901

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin's lymphoma.

Target Information

The MUM1 (Multiple Myeloma Oncogene 1) gene was originally identified because of its involvement in the t(6;14) translocation observed in multiple myeloma, which causes juxtaposition of the MUM1 gene to the Ig heavy-chain locus. MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloid and extra-hematopoitic tumors. MUM1 is a valuable marker for understanding and characterizing histogenesis of B-cell lymphomas. It is an excellent marker for Reed-Sternberg cells of classic Hodgkin's disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5

Gene Aliases: IRF4; LSIRF; MUM1; NF-EM5; SHEP8

UniProt ID: (Human) Q15306

Entrez Gene ID: (Human) 3662

Molecular Function: nucleic acid binding transcription factor