Flow cytometry analysis of CD11a in mouse splenocytes (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # MA11A5) at a dilution of 0.5 ug/test. Cells were incubated for 30 min at 4°C and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4°C in the dark. FACS analysis was performed using 400 ul of cell buffer.
|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Inhibition Assays (IA)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA11A5 targets CD11a in FACS, IA, and Neu applications and shows reactivity with mouse samples. This antibody does not react with human Jurkat cells in FACS applications.
The MA11A5 immunogen is mouse 180kD alpha chain (CD11alpha) of the integrin Lymphocyte Function-Associated Antigen-1.
MA11A5 detects CD11a which has a predicted molecular weight of approximately 126 kDa.
This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.
This product is a Low Endotoxin formulation.
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tec kinases regulate TCR-mediated recruitment of signaling molecules and integrin-dependent cell adhesion.
MA11A5 was used in western blot to investigate the role of Tec kinases in regulating integrin-mediated adhesion and recruitment of signaling complexes
|Finkelstein LD,Shimizu Y,Schwartzberg PL||Journal of immunology (Baltimore, Md. : 1950) (175:5923)||2005|
Syk is required for integrin signaling in neutrophils.
MA11A5 was used in blocking or activating experiment to study the role of Syk tyrosine kinase in integrin signaling in neutrophils
|Mócsai A,Zhou M,Meng F,Tybulewicz VL,Lowell CA||Immunity (16:547)||2002|
Monoclonal antibodies that block the activity of leukocyte function-associated antigen 1 recognize three discrete epitopes in the inserted domain of CD11a.
MA11A5 was used in immunoprecipitation to study the inhibitory properties of eight monoclonal antibodies to leukocyte function-associated antigen against mutated forms of human CD11a.
|Champe M,McIntyre BW,Berman PW||The Journal of biological chemistry (270:1388)||1995|