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Flow cytometry analysis of CD49d in THP-1 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD49d monoclonal antibody (Product # MA49D7) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Rat 150 kD alpha4 chain of VLA-4 (CD49d).|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Neutralization (Neu)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA49D7 targets CD49d in FACS, IHC, and Neu applications and shows reactivity with Rat and Human samples.
The MA49D7 immunogen is rat 150 kD alpha4 chain of VLA-4 (CD49d).
MA49D7 detects CD49d which has a predicted molecular weight of approximately 111 kDa.
The product of this gene belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an alpha 4 chain. Unlike other integrin alpha chains, alpha 4 neither contains an I-domain, nor undergoes disulfide-linked cleavage. Alpha 4 chain associates with either beta 1 chain or beta 7 chain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Resting CD4+ effector memory T cells are precursors of bystander-activated effectors: a surrogate model of rheumatoid arthritis synovial T-cell function.
MA49D7 was used in blocking/activating experiment to study the role of resting CD4+ effector memory T cells in rheumatoid arthritis
|Brennan FM,Smith NM,Owen S,Li C,Amjadi P,Green P,Andersson A,Palfreeman AC,Hillyer P,Foey A,Beech JT,Feldmann M||Arthritis research & therapy (10:null)||2008|
VCAM-1 contributes to rapid eosinophil accumulation induced by the chemoattractants PAF and LTB4: evidence for basal expression of functional VCAM-1 in rat skin.
MA49D7 was used in blocking/activating experiment to study the role of VCAM-1 in eosinophil accumulation
|Davies D,Larbi K,Allen A,Sanz M,Weg VB,Haskard DO,Lobb RR,Nourshargh S||Immunology (97:150)||1999|
Inhibition of in vivo lymphocyte migration to inflammation and homing to lymphoid tissues by the TA-2 monoclonal antibody. A likely role for VLA-4 in vivo.
MA49D7 was used in blocking/activating experiment to investigate the role of VLA-4 in the migration of lymphocyte under inflammation conditions
|Issekutz TB||Journal of immunology (Baltimore, Md. : 1950) (147:4178)||1991|
VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.
MA49D7 was used in ELISA to investigate the role of endothelial-cell VCAM-1 in eosinophil accumulation
|Larbi KY,Allen AR,Tam FW,Haskard DO,Lobb RR,Silva PM,Nourshargh S||Blood (96:3601)||2000|
Lymphocyte subpopulations in lymphoid organs of rats after acute resistance exercise.
MA49D7 was used in flow cytometry to investigate the changes of lymphocyte subpopulations in vivo with acute resistance exercise
|Mastro AM,Schlosser DA,Grove DS,Lincoski C,Pishak SA,Gordon S,Kraemer WJ||Medicine and science in sports and exercise (31:74)||1999|
Effect of a new monoclonal antibody, TA-2, that inhibits lymphocyte adherence to cytokine stimulated endothelium in the rat.
MA49D7 was used in immunoprecipitation to study the lymphocyte and endothelium adhesion in rats
|Issekutz TB,Wykretowicz A||Journal of immunology (Baltimore, Md. : 1950) (147:109)||1991|
269C wild type; alpha 4 subunit of VLA-4 receptor; alpha-4 subunit of VLA-4 receptor; antigen CD49D; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; integrin alpha-IV; ITGA-IV; ITGA4; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha
CD49D; IA4; ITGA4